Results 171 to 180 of about 1,649,089 (269)

The photoswitchable cannabinoid azo‐HU308 enables optical control of Ca2+ dynamics in INS‐1 β‐cells via off‐target effects on TRPC channels

open access: yesFEBS Open Bio, EarlyView.
Light activation of the photoswitchable cannabinoid ligand azo‐HU308 triggers Ca2+ influx in pancreatic β‐cells through TRPC channels, independent of CB2 cannabinoid receptors. This reveals a non‐GPCR pathway for cannabinoid modulation of β‐cell Ca2+ dynamics and establishes azo‐HU308 as an optical tool to study cannabinoid signaling through TRP ...
Alexander E. G. Viray, James A. Frank
wiley   +1 more source

Recovery of IR700 Fluorescence After Near-Infrared Photoimmunotherapy: Discovery and Mechanistic Insights. [PDF]

open access: yesCancers (Basel)
Tanaka H   +9 more
europepmc   +1 more source

Electrochemically Stimulated Autofluorescence of Indium Tin Oxide Surface: The Implications for Fluorescence Microscopy

open access: green
Daniel Hagness   +6 more
openalex   +2 more sources

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

open access: yesFEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq   +3 more
wiley   +1 more source

Monitoring Macrophage Polarization with Gene Expression Reporters and Bioluminescence Phasor Analysis

open access: yesChemical & Biomedical Imaging
Giulia Tedeschi   +5 more
doaj   +1 more source

7A: MitoTracker red fluorescence images at both 20x and 40x by confocal microscopy for two independent experiments.

open access: green
Lauren A. Eanes (20995499)   +3 more
openalex   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

open access: yesFEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito   +3 more
wiley   +1 more source

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