Results 71 to 80 of about 3,329,276 (417)

An Intermediate State of the {gamma}-Aminobutyric Acid Transporter GAT1 Revealed by Simultaneous Voltage Clamp and Fluorescence [PDF]

open access: yes, 2000
The rat {gamma}-aminobutyric acid transporter GAT1 expressed in Xenopus oocytes was labeled at Cys74, and at one or more other sites, by tetramethylrhodamine-5-maleimide, without significantly altering GAT1 function.
Farley, Robert A.   +2 more
core   +1 more source

Screening of antioxidant properties of the apple juice using the front-face synchronous fluorescence and chemometrics [PDF]

open access: yes, 2017
Fluorescence spectroscopy is gaining increasing attention in food analysis due to its higher sensitivity and selectivity as compared to other spectroscopic techniques. Synchronous scanning fluorescence technique is particularly useful in studies of multi-
A Gliszczyńska-Świgło   +52 more
core   +1 more source

Unraveling Mycobacterium tuberculosis acid resistance and pH homeostasis mechanisms

open access: yesFEBS Letters, EarlyView.
Mycobacterium tuberculosis exhibits a remarkable resilience to acid stress. In this Review, we discuss some of the molecular mechanisms and metabolic pathways used by the tubercle bacilli to adapt and resist host‐mediated acid stress. Mycobacterium tuberculosis (Mtb) is a successful pathogen that has developed a variety of strategies to survive and ...
Janïs Laudouze   +3 more
wiley   +1 more source

Photophysics, Molecular Reorientation in Solution and X-Ray Structure of a New Fluorescent Probe 1,7-Diazaperylene [PDF]

open access: yes, 1993
A new fluorescent molecule 1,7-diazaperylene (DP) has been investigated by means of time-resolved and steady-state polarized fluorescence spectroscopy, as well as X-ray spectroscopy.
Barkely   +32 more
core   +1 more source

Fluorescence Microscopy [PDF]

open access: yesCold Spring Harbor Protocols, 2014
Fluorescence microscopy is a major tool with which to monitor cell physiology. Although the concepts of fluorescence and its optical separation using filters remain similar, microscope design varies with the aim of increasing image contrast and spatial resolution.
Sanderson, Michael J   +3 more
openaire   +3 more sources

Chlorophyll a fluorescence as a tool to monitor physiological status of plants under abiotic stress conditions

open access: yesActa Physiologiae Plantarum, 2016
Plants living under natural conditions are exposed to many adverse factors that interfere with the photosynthetic process, leading to declines in growth, development, and yield.
H. Kalaji   +9 more
semanticscholar   +1 more source

Refining the NaV1.7 pharmacophore of a class of venom‐derived peptide inhibitors via a combination of in silico screening and rational engineering

open access: yesFEBS Letters, EarlyView.
Venom peptides have shown promise in treating pain. Our study uses computer screening to identify a peptide that targets a sodium channel (NaV1.7) linked to chronic pain. We produced the peptide in the laboratory and refined its design, advancing the search for innovative pain therapies.
Gagan Sharma   +8 more
wiley   +1 more source

Theoretical studies on triplet formations in nitrobenzoxadiazole (NBD) derivatives: The impact of donor group and heteroatom substitution

open access: yesResults in Chemistry, 2021
Heavy-atom-free triplet photosensitizers (TPs) have attracted much interest due to the applications in triplet–triplet annihilation (TTA) and photodynamic therapy.
Chao Wang   +3 more
doaj  

In Vivo Single-Cell Detection of Metabolic Oscillations in Stem Cells

open access: yesCell Reports, 2015
Through the use of bulk measurements in metabolic organs, the circadian clock was shown to play roles in organismal energy homeostasis. However, the relationship between metabolic and circadian oscillations has not been studied in vivo at a single-cell ...
Chiara Stringari   +7 more
doaj   +1 more source

Label-free prediction of three-dimensional fluorescence images from transmitted light microscopy

open access: yesNature Methods, 2018
Understanding cells as integrated systems is central to modern biology. Although fluorescence microscopy can resolve subcellular structure in living cells, it is expensive, is slow, and can damage cells.
Chawin Ounkomol   +4 more
semanticscholar   +1 more source

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