Results 21 to 30 of about 129,094 (159)

Detection of Individual RNA in Fixed Cells and Tissues by Chromogenic ISH

Bio-Protocol, 2020
Visualization of RNA molecules in situ helps to better understand the functions of expressed genes. Currently, most conventional in situ hybridization methods for visualization of individual RNAs are based on fluorescence detection.
Meng Jiang, Chen Lin, Rongqin Ke
doaj   +1 more source

Multiplexed Single-Cell in situ RNA Profiling

Frontiers in Molecular Biosciences, 2021
The ability to quantify a large number of varied transcripts in single cells in their native spatial context is crucial to accelerate our understanding of health and disease.
Yu-Sheng Wang, Jia Guo
doaj   +1 more source

Transcriptional network analysis of PTEN‐protein‐deficient prostate tumors reveals robust stromal reprogramming and signs of senescent paracrine communication

Molecular Oncology, EarlyView.
Combining PTEN protein assessment and transcriptomic profiling of prostate tumors, we uncovered a network enriched in senescence and extracellular matrix (ECM) programs associated with PTEN loss and conserved in a mouse model. We show that PTEN‐deficient cells trigger paracrine remodeling of the surrounding stroma and this information could help ...
Ivana Rondon‐Lorefice, Jose I. Lopez, Aitziber Ugalde‐Olano, Maite Zufiaurre, Ianire Astobiza, Natalia Martin‐Martin, Laura Bozal‐Basterra, Saioa Garcia‐Longarte, Amaia Zabala‐Letona, Sofia Rey, Aida Santos‐Martin, Miguel Unda, Ana Loizaga‐Iriarte, Mariona Graupera, Paolo Nuciforo, Arkaitz Carracedo, Isabel Mendizabal   +16 more
wiley   +1 more source

Biological implications of deletion p53 by fluorescence in situ hybridization in multiple myeloma

Biomedical and Biotechnology Research Journal, 2022
Background: Multiple myeloma (MM) is a clonal plasma cell disorder characterized by heterogeneous complex genetic abnormalities. Due to the low proliferative index of plasma cells, conventional cytogenetic (CC) analysis is hampered in MM.
Vijith Vittal Shetty, Meenakshi Arumugam, Reshma Arun Shetty, Akanksha Ashok Kalal, Nagaraj Venkatesh Kulkarni, Deyyenthody Prashanth Shetty   +5 more
doaj   +1 more source

LINC01116, a hypoxia‐lncRNA marker of pathological lymphangiogenesis and poor prognosis in lung adenocarcinoma

Molecular Oncology, EarlyView.
The LINC01116 long noncoding RNA is induced by hypoxia and associated with poor prognosis and high recurrence rates in two cohorts of lung adenocarcinoma patients. Here, we demonstrate that besides its expression in cancer cells, LINC01116 is markedly expressed in lymphatic endothelial cells of the tumor stroma in which it participates in hypoxia ...
Marine Gautier‐Isola, Rafael Lopes Goncalves, Marin Truchi, Caroline Lacoux, Célia Scribe, Hugo Cadis, Laetitia Guardini, Sophie Bekisz, Marius Ilié, Paul Hofman, Georges Vassaux, Bernard Mari, Roger Rezzonico   +12 more
wiley   +1 more source

Cytogenetic follow-up by karyotyping and fluorescence in situ hybridization: implications for monitoring patients with myelodysplastic syndrome and deletion 5q treated with lenalidomide

Haematologica, 2011
In patients with low and intermediate risk myelodysplastic syndrome and deletion 5q (del(5q)) treated with lenalidomide, monitoring of cytogenetic response is mandatory, since patients without cytogenetic response have a significantly increased risk of ...
Gudrun Göhring, Aristoteles Giagounidis, Guntram Büsche, Winfried Hofmann, Hans Heinrich Kreipe, Pierre Fenaux, Eva Hellström-Lindberg, Brigitte Schlegelberger   +7 more
doaj   +1 more source

Crucial parameters for precise copy number variation detection in formalin‐fixed paraffin‐embedded solid cancer samples

Molecular Oncology, EarlyView.
This study shows that copy number variations (CNVs) can be reliably detected in formalin‐fixed paraffin‐embedded (FFPE) solid cancer samples using ultra‐low‐pass whole‐genome sequencing, provided that key (pre)‐analytical parameters are optimized.
Hanne Goris, Vasiliki Siozopoulou, Léon C van Kempen, Anne Sieben, Ella Roelant, Stig Hellemans, Elyne Backx, Laure Sorber, Koen De Winne, Senada Koljenović, Karen Zwaenepoel   +10 more
wiley   +1 more source

Drawback of chimerism analysis by XY-fluorescence in situ hybridization: Deception of a relapse

Indian Journal of Medical and Paediatric Oncology, 2020
Chimerism is monitored by various methods in posttransplant patients. Interphase fluorescence in situ hybridization (FISH) for XY (sex) chromosomes is one of the methods commonly employed.
D VS Kiran Kumar, Jayachandran Perumal Kalaiyarasi   +1 more
doaj   +1 more source

Electropermeabilization-based fluorescence in situ hybridization of whole-mount plant parasitic nematode specimens

MethodsX, 2019
A fluorescence in situ hybridization (FISH) protocol was developed for nematodes in which nucleic acid probes are introduced within the organism via electroporation.
Casey L. Ruark-Seward, Eric L. Davis, Tim L. Sit   +2 more
doaj   +1 more source

Advances and Challenges in Fluorescence in situ Hybridization for Visualizing Fungal Endobacteria

Frontiers in Microbiology, 2022
Several bacteria have long been known to interact intimately with fungi, but molecular approaches have only recently uncovered how cosmopolitan these interactions are in nature.
Demosthenes P. Morales, Aaron J. Robinson, Andrew C. Pawlowski, Andrew C. Pawlowski, Caitlyn Ark, Julia M. Kelliher, Pilar Junier, James H. Werner, Patrick S. G. Chain   +8 more
doaj   +1 more source