A Technical Note on Quantum Dots for Multi-Color Fluorescence in situ Hybridization [PDF]
Quantum dots (Qdots) are semiconductor nanocrystals, which are photo-stable, show bright fluorescence with narrow, symmetric emission spectra and are available in multiple resolvable colors.
Cremer, M.+4 more
core +1 more source
Biological implications of deletion p53 by fluorescence in situ hybridization in multiple myeloma
Background: Multiple myeloma (MM) is a clonal plasma cell disorder characterized by heterogeneous complex genetic abnormalities. Due to the low proliferative index of plasma cells, conventional cytogenetic (CC) analysis is hampered in MM.
Vijith Vittal Shetty+5 more
doaj +1 more source
Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women
The aim of this study was to use the fluorescence in situ hybridization (FISH) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women.
Fernanda Campos Machado+4 more
doaj +1 more source
Characterization of two marker chromosomes in a patient with acute nonlymphocytic leukemia by two-color fluorescence in situ hybridization [PDF]
A patient with acute nonlymphocytic leukemia (ANLL), M5b according to French-American-British (FAB) classification, showed monosomy 16, an extra 1p−, and a 21q+. These derivative chromosomes could not be defined by GTG-banding.
Anna Jauch+26 more
core +1 more source
Fluorescent in Situ Hybridization
Abstract not availableBangladesh J Med Biochem 2017; 10(1): 3 ...
S Akhter, HB Asad, Z Aziz
openaire +3 more sources
Detection of chromosome aberrations in the human interphase nucleus by visualization of specific target DNAs with radioactive and non-radioactive in situ hybridization techniques: diagnosis of trisomy 18 with probe L1.84 [PDF]
The localization of chromosome 18 in human interphase nuclei is demonstrated by use of radioactive and nonradioactive in situ hybridization techniques with a DNA clone designated L1.84.
A. Brückner+33 more
core +1 more source
A modified protocol for the detection of three different mRNAs with a new-generation in situ hybridization chain reaction on frozen sections [PDF]
A new multiple fluorescence in situ hybridization method based on hybridization chain reaction was recently reported, enabling simultaneous mapping of multiple target mRNAs within intact zebrafish and mouse embryos.
Burnstock, G+7 more
core
Autofluorescence correction for fluorescence in situ hybridization [PDF]
AbstractOptimal sensitivity of fluorescence in situ hybridization (FISH) requires bright signals and low background fluorescence. Use of locus‐specific probes is especially dependent on high sensitivity. Some tissue preparations show high autofluorescence, masking small or dim signals.
Szöllősi, János+3 more
openaire +3 more sources
Urine is a rich source of biomarkers for cancer detection. Tumor‐derived material is released into the bloodstream and transported to the urine. Urine can easily be collected from individuals, allowing non‐invasive cancer detection. This review discusses the rationale behind urine‐based cancer detection and its potential for cancer diagnostics ...
Birgit M. M. Wever+1 more
wiley +1 more source
In patients with low and intermediate risk myelodysplastic syndrome and deletion 5q (del(5q)) treated with lenalidomide, monitoring of cytogenetic response is mandatory, since patients without cytogenetic response have a significantly increased risk of ...
Gudrun Göhring+7 more
doaj +1 more source