Background Triple-negative breast carcinoma (TNBC) patients do not benefit from hormone- or human epidermal growth factor receptor 2- (HER2-) targeted therapies. Accurate testing is pivotal for these patients. Methods TNBC cases that were retested at our
Jose De Jesus, Marilin Rosa
doaj +1 more source
Multicolor fluorescence in situ hybridization on metaphase chromosomes and interphase Halo-preparations using cosmid and YAC clones for the simultaneous high resolution mapping of deletions in the dystrophin gene [PDF]
We report on multicolor fluorescence in situ hybridization protocols for the simultaneous visualization of deletion-prone regions for carrier detection of Duchenne/ Becker (DMD/BMD) muscular dystrophy.
Blonden, Lau+6 more
core +1 more source
Recent Advances in Highly Luminescent Carbon Dots
Here, a comprehensive summary of the advancements is provided in highly luminescent carbon dots across various luminescence bands since their discovery in 2004. It systematically covers the synthesis, structural characteristics, preparation strategies, and essential purification of these carbon dots, along with their applications in efficient ...
Yupeng Liu+6 more
wiley +1 more source
Highly Sensitive and Multiplexed In Situ RNA Profiling with Cleavable Fluorescent Tyramide
Understanding the composition, regulation, and function of complex biological systems requires tools that quantify multiple transcripts at their native cellular locations.
Lu Xiao, Joshua Labaer, Jia Guo
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A Technical Note on Quantum Dots for Multi-Color Fluorescence in situ Hybridization [PDF]
Quantum dots (Qdots) are semiconductor nanocrystals, which are photo-stable, show bright fluorescence with narrow, symmetric emission spectra and are available in multiple resolvable colors.
Cremer, M.+4 more
core +1 more source
Making Photoresponsive Metal–Organic Frameworks an Effective Class of Heterogeneous Photocatalyst
This review summarizes photoresponsive MOFs for photocatalytic applications, focusing on their capacity to enhance light harvesting, charge transfer, and surface reactions. While existing studies provide foundational insights, emerging characterization techniques enable a deeper understanding of photoresponsive MOFs.
Rui Liu+3 more
wiley +1 more source
Characterization of two marker chromosomes in a patient with acute nonlymphocytic leukemia by two-color fluorescence in situ hybridization [PDF]
A patient with acute nonlymphocytic leukemia (ANLL), M5b according to French-American-British (FAB) classification, showed monosomy 16, an extra 1p−, and a 21q+. These derivative chromosomes could not be defined by GTG-banding.
Anna Jauch+26 more
core +1 more source
A pipeline for targeted metagenomics of environmental bacteria. [PDF]
BackgroundMetagenomics and single cell genomics provide a window into the genetic repertoire of yet uncultivated microorganisms, but both methods are usually taxonomically untargeted.
Bowers, Robert M+7 more
core +2 more sources
MXene 3D/4D Printing: Ink Formulation and Electrochemical Energy Storage Applications
A comprehensive study of MXene printing technique is presented, focusing on MXene ink formulation, surface chemistry, rheological characteristics, physical storage, and ink stability. Different printing techniques, such as 3D/4D printing, screen printing, inkjet printing, and continuous liquid interface production printing along with their applications
Shaista Nouseen, Martin Pumera
wiley +1 more source
Fluorescence In Situ Hybridization Method for Measuring Transfection Efficiency
We describe here the use of fluorescence in situ hybridization (FISH) to measure the transfection efficiency of the transient expression vector pCMVcat in lymphoblasts and fibroblasts.
Lie Cheng, Corazon D. Bucana, Qingyi Wei
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