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Confocal Fluorescence Lifetime Imaging
1996The optical microscope, arguably the most mature of all laboratory instruments, is currently enjoying a period of intense development that is opening up many new application areas. These developments have been made possible by the use of new optical arrangements, sophisticated light sources and detectors and by the application of novel fluorescence ...
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FLUORESCENCE LIFETIMES OF ANGULAR FUROCOUMARINS
Photochemistry and Photobiology, 1987AbstractProperties of singlet states of a range of non‐substituted and aceto‐substituted angular furocoumarins as investigated by the technique of time‐resolved fluorescence are reported. In the three solvents studied, water, ethanol and benzene, a variety of 1, 2 or 3 fluorescence lifetime components were found and resolved, the importance of which ...
Andreoni A +3 more
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Fluorescence lifetimes: fundamentals and interpretations
Photosynthesis Research, 2009Fluorescence measurements have been an established mainstay of photosynthesis experiments for many decades. Because in the photosynthesis literature the basics of excited states and their fates are not usually described, we have presented here an easily understandable text for biology students in the style of a chapter in a text book. In this review we
Ulai, Noomnarm, Robert M, Clegg
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Fourier Multiplexed Fluorescence Lifetime Imaging
2021Fluorescence lifetime imaging microscopy (FLIM) is a widely used functional imaging method in bioscience. Fourier multiplexed FLIM (FmFLIM), a frequency-domain lifetime measurement method, explores the principle of Fourier (frequency) multiplexing to achieve parallel lifetime detection on multiple fluorescence labels.
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Fluorescence lifetimes of Chlorella pyrenoidosa
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1976The flucrescence decay of Chlorella pyrenoidosa has been investigated under a variety of conditions in the picosecond and nanosecond time regions. Most of the fluorescence is accounted for by an expression of the form I(t) = I0exp-(At+Bt1/2) though an additional exponential term is required to include a weak component of lifetime 32 ps observable only ...
L, Harris +4 more
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Fluorescence Lifetime Imaging Microscopy (FLIM)
2005Fluorescence lifetime imaging microscopy (FLIM) is a technique to map the spatial distribution of nanosecond excited state lifetimes within microscopic images. FLIM systems have been implemented both in the frequency domain, using sinusoidally intensity-modulated excitation light and modulated detectors, and in the time domain, using pulsed excitation ...
Erik B, van Munster +1 more
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Fluorescence Lifetime Tomography
European Conferences on Biomedical Optics 2025Time-resolved fluorescence tomography enables precise spatial differentiation of fluorophores with overlapping spectra. Using a scanning system, we acquired decay kinetics from a sample phantom filled with acridine orange and fluorescein, revealing distinct lifetime contrasts.
Fabian Rieder +3 more
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FLUORESCENCE LIFETIME OF PHYCOERYTHRIN*
Photochemistry and Photobiology, 1968F, Macdowall, M, Walker
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