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Fluorescence Lifetime Imaging Microscopy
Current Protocols in Cell Biology, 2004AbstractFluorescent lifetime imaging microscopy is a powerful tool to enhance the contrast in images of biological samples and to investigate the local environment of a fluorochrome. FLIM allows the detection of protein‐protein interactions and their biochemical state by the quantitative detection of Förster resonance energy transfer (FRET) between ...
Alessandro, Esposito, Fred S, Wouters
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Fluorescence lifetime-resolved imaging
Photosynthesis Research, 2009This is a short account of fluorescence lifetime-resolved imaging, in order to acquaint readers who are not experts with the basic methods for measuring lifetime-resolved signals throughout an image. We present the early FLI (fluorescence lifetime imaging) history, review shortly the instrumentation and experimental design, discuss briefly the ...
Yi-Chun, Chen, Robert M, Clegg
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Fluorescence Lifetime Correlation Spectroscopy
Journal of Fluorescence, 2006This article explains the basic principles of FLCS, a genuine fusion of Time-Correlated Single Photon Counting (TCSPC) and Fluorescence Correlation Spectroscopy (FCS), using common terms and minimum mathematics. The usefulness of the method is demonstrated on simple FCS experiments.
Peter, Kapusta +4 more
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Fluorescence Lifetime Imaging Microscopy
2007Publisher Summary Fluorescence lifetime imaging microscopy (FLIM) produces spatially resolved images of fluorophore lifetime (the property describing how rapidly fluorescence decays), providing another dimension of information for visualizing fluorophores and an additional source of contrast.
Ching-Wei, Chang +2 more
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Fluorescence lifetime imaging of coral fluorescent proteins
Microscopy Research and Technique, 2007AbstractCorals, like many other coelenterates, contain fluorescent pigments that show considerable homology with the well known green fluorescent protein of the jellyfish Aequoria. In corals, unlike jellyfish, multiple proteins are present and the range of excitations and emissions suggest the possibility of energy transfer.
Guy, Cox, Mikhail, Matz, Anya, Salih
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Fluorescence lifetime based bioassays
Methods and Applications in Fluorescence, 2017Fluorescence lifetime (FLT) is a robust intrinsic property and material constant of fluorescent matter. Measuring this important physical indicator has evolved from a laboratory curiosity to a powerful and established technique for a variety of applications in drug discovery, medical diagnostics and basic biological research.
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2006
A newly developed fluorescence lifetime imaging microscopy (FLIM) system has combined the high-temporal resolution of a streak camera with the high-spatial resolution of a microscope to obtain a two-dimensional distribution of fluorescence lifetimes within living cells. The temporal resolution is as short as 20 ps.
Masanobu, Fujiwara, William, Cieslik
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A newly developed fluorescence lifetime imaging microscopy (FLIM) system has combined the high-temporal resolution of a streak camera with the high-spatial resolution of a microscope to obtain a two-dimensional distribution of fluorescence lifetimes within living cells. The temporal resolution is as short as 20 ps.
Masanobu, Fujiwara, William, Cieslik
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2014
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is largely independent of the local fluorophore concentration and excitation ...
Suhling, Klaus +11 more
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Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is largely independent of the local fluorophore concentration and excitation ...
Suhling, Klaus +11 more
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Rapid hyperspectral fluorescence lifetime imaging
Microscopy Research and Technique, 2007AbstractWe report a rapid hyperspectral fluorescence lifetime imaging (FLIM) instrument that exploits high‐speed FLIM technology in a line‐scanning microscope. We demonstrate the acquisition of whole‐field optically sectioned hyperspectral fluorescence lifetime image stacks (with 32 spectral bins) in less than 40 s and illustrate its application to ...
Pieter, De Beule +13 more
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Three‐dimensional fluorescence lifetime tomography
Medical Physics, 2005Near‐infrared fluorescence tomography using molecularly targeted lifetime‐sensitive, fluorescent contrast agents have applications for early‐stage cancer diagnostics. Yet, although the measurement of fluorescent lifetime imaging microscopy (FLIM) is extensively used in microscopy and spectroscopy applications, demonstration of fluorescence lifetime ...
Anuradha, Godavarty +2 more
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