Results 1 to 10 of about 36,842 (272)

Real-time open-source FLIM analysis

Frontiers in Bioinformatics, 2023
Fluorescence lifetime imaging microscopy (FLIM) provides valuable quantitative insights into fluorophores’ chemical microenvironment. Due to long computation times and the lack of accessible, open-source real-time analysis toolkits, traditional analysis ...
Kevin W Eliceiri
exaly   +4 more sources

FLIM as a Promising Tool for Cancer Diagnosis and Treatment Monitoring

Nano-Micro Letters, 2021
Fluorescence lifetime imaging microscopy (FLIM) has been rapidly developed over the past 30 years and widely applied in biomedical engineering. Recent progress in fluorophore-dyed probe design has widened the application prospects of fluorescence ...
Yanping Liu, Zongwen Liu, Ming-Hua Wu
exaly   +3 more sources

Investigating Origins of FLIm Contrast in Atherosclerotic Lesions Using Combined FLIm-Raman Spectroscopy [PDF]

Frontiers in Cardiovascular Medicine, 2020
Background: Fluorescence lifetime imaging (FLIm) is a spectroscopic imaging technique able to characterize the composition of luminal surface of arterial vessels.
Julien Bec, Christoph Krafft, T W Bocklitz   +2 more
exaly   +7 more sources

Fast volumetric fluorescence lifetime imaging of multicellular systems using single-objective light-sheet microscopy [PDF]

Communications Biology
Fluorescence lifetime imaging (FLIM) is widely used for functional and multiplexed bioimaging. The lifetime of autofluorescence or fluorescent sensors encodes physiologically relevant parameters. Thus, FLIM is especially relevant for the investigation of
Valentin Dunsing-Eichenauer, Johan Hummert, Claire Chardès, Thomas Schönau, Léo Guignard, Rémi Galland, Gianluca Grenci, Max Tillmann, Felix Koberling, Corinna Nock, Jean-Baptiste Sibarita, Virgile Viasnoff, Ivan Michel Antolovic, Rainer Erdmann, Pierre-François Lenne   +14 more
doaj   +2 more sources

Investigating probe-receptor interactions and enhancing fluorescence guided surgery with fluorescence lifetime imaging and NF-800 in HER-2 positive esophageal adenocarcinoma [PDF]

Scientific Reports
FLIM (Fluorescence Lifetime Imaging Microscopy) is a powerful technique used to study protein–protein interactions, enzyme kinetics dynamics, and cellular metabolism.
Francesca La Cava, Francesca Arena, Daniele Faletto, Margherita Iaboni, Federico Crivellin, Alessia Cordaro, Miriam Roberto, Federica Pozzi, Francesco Fersini, Giovanni Valbusa, Francesco Stummo, Mathieu Cinier, Simon Huet, Francesco Blasi, Alessandro Maiocchi, Luisa Poggi, Erika Reitano   +16 more
doaj   +2 more sources

The Small Intestine in the Acute Period of Spinal Injury: Early Metabolic Disorders According to Fluorescence-Lifetime Imaging FLIM

Неотложная медицинская помощь, 2023
RELEVANCE A special place in the development of enteral insufficiency is given to dysproteinemia, which is one of the leading causes of the development of decubital ulcers in patients with spinal cord injury. Early enteral nutrition partially solved this
M. S. Baleyev, E. B. Kiseleva, M. M. Loginova, M. V. Shirmanova, A. P. Fraerman, V. I. Shcheslavskiy, N. D. Gladkova, M. G. Ryabkov   +7 more
doaj   +1 more source

Quantitative FRET-FLIM-BlaM to Assess the Extent of HIV-1 Fusion in Live Cells

Viruses, 2020
The first steps of human immunodeficiency virus (HIV) infection go through the engagement of HIV envelope (Env) with CD4 and coreceptors (CXCR4 or CCR5) to mediate viral membrane fusion between the virus and the host.
Irene Carlon-Andres, Sergi Padilla-Parra
doaj   +1 more source

Simple and Robust Deep Learning Approach for Fast Fluorescence Lifetime Imaging

Sensors, 2022
Fluorescence lifetime imaging (FLIM) is a powerful tool that provides unique quantitative information for biomedical research. In this study, we propose a multi-layer-perceptron-based mixer (MLP-Mixer) deep learning (DL) algorithm named FLIM-MLP-Mixer ...
Quan Wang, Yahui Li, Dong Xiao, Zhenya Zang, Zi’ao Jiao, Yu Chen, David Day Uei Li   +6 more
doaj   +1 more source

First results of computer-enhanced optical diagnosis of bladder cancer

Current Directions in Biomedical Engineering, 2020
Bladder cancer is the sixth leading cancer cause worldwide. Non-muscle invasive tumors can be diagnosed and treated endoscopically. Based on biopsies alone, pathologists cannot determine the spatial organization of specimens, their relationship with each
Wittenberg T., Hackner R., Bocklitz T., Krafft C., Becker W., Braun L., Pohlmann P., Miernik A., Suarez-Ibarrola R., Lemke M. N.   +9 more
doaj   +1 more source

Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy

Scientific Reports, 2021
We demonstrate that structured illumination microscopy has the potential to enhance fluorescence lifetime imaging microscopy (FLIM) as an early detection method for oral squamous cell carcinoma.
Taylor A. Hinsdale, Bilal H. Malik, Shuna Cheng, Oscar R. Benavides, Maryellen L. Giger, John M. Wright, Paras B. Patel, Javier A. Jo, Kristen C. Maitland   +8 more
doaj   +1 more source