Results 1 to 10 of about 9,845 (208)
Real-time open-source FLIM analysis
Frontiers in Bioinformatics, 2023 Fluorescence lifetime imaging microscopy (FLIM) provides valuable quantitative insights into fluorophores’ chemical microenvironment. Due to long computation times and the lack of accessible, open-source real-time analysis toolkits, traditional analysis ...
Kevin Eliceiri, Jenu V. Chacko
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FLIM as a Promising Tool for Cancer Diagnosis and Treatment Monitoring
Nano-Micro Letters, 2021 Fluorescence lifetime imaging microscopy (FLIM) has been rapidly developed over the past 30 years and widely applied in biomedical engineering. Recent progress in fluorophore-dyed probe design has widened the application prospects of fluorescence ...
Ming-Hua Wu, Yanping Liu, Zongwen Liu
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FLIMExplorer: interactive GUI for object-based visualization and analysis of fluorescence lifetime images [PDF]
JPhys PhotonicsFluorescence lifetime imaging microscopy (FLIM) enables non-invasive measurement of cellular metabolism with single-cell and subcellular resolution, providing advantages over traditional population-level metabolic assays.
Blanche ter Hofstede +5 more
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Неотложная медицинская помощь, 2023 RELEVANCE A special place in the development of enteral insufficiency is given to dysproteinemia, which is one of the leading causes of the development of decubital ulcers in patients with spinal cord injury. Early enteral nutrition partially solved this
M. S. Baleyev +7 more
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Quantitative FRET-FLIM-BlaM to Assess the Extent of HIV-1 Fusion in Live Cells
Viruses, 2020 The first steps of human immunodeficiency virus (HIV) infection go through the engagement of HIV envelope (Env) with CD4 and coreceptors (CXCR4 or CCR5) to mediate viral membrane fusion between the virus and the host.
Irene Carlon-Andres, Sergi Padilla-Parra
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Simple and Robust Deep Learning Approach for Fast Fluorescence Lifetime Imaging
Sensors, 2022 Fluorescence lifetime imaging (FLIM) is a powerful tool that provides unique quantitative information for biomedical research. In this study, we propose a multi-layer-perceptron-based mixer (MLP-Mixer) deep learning (DL) algorithm named FLIM-MLP-Mixer ...
Quan Wang +6 more
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First results of computer-enhanced optical diagnosis of bladder cancer
Current Directions in Biomedical Engineering, 2020 Bladder cancer is the sixth leading cancer cause worldwide. Non-muscle invasive tumors can be diagnosed and treated endoscopically. Based on biopsies alone, pathologists cannot determine the spatial organization of specimens, their relationship with each
Wittenberg T. +9 more
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Phasor identifier: A cloud-based analysis of phasor-FLIM data on Python notebooks
Biophysical Reports, 2023 This paper introduces an innovative approach utilizing Google Colaboratory for the versatile analysis of phasor fluorescence lifetime imaging microscopy (FLIM) data collected from various samples (e.g., cuvette, cells, tissues) and in various input file ...
Mario Bernardi, Francesco Cardarelli
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Scientific Reports, 2021 We demonstrate that structured illumination microscopy has the potential to enhance fluorescence lifetime imaging microscopy (FLIM) as an early detection method for oral squamous cell carcinoma.
Taylor A. Hinsdale +8 more
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Metabolic Imaging by Simultaneous FLIM of NAD(P)H and FAD
Current Directions in Biomedical Engineering, 2020 We describe a metabolic imaging system based on simultaneous recording of lifetime images of NAD(P)H and FAD. The system uses one-photon excitation by ps diode lasers, scanning by galvanometer mirrors, confocal detection, and two parallel TCSPC FLIM ...
Becker Wolfgang +3 more
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