Results 41 to 50 of about 36,842 (272)

A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos

Scientific Reports
A wide-field microscope with epi-fluorescence and selective plane illumination was combined with a single-photon avalanche diode (SPAD) array camera to enable live-cell fluorescence lifetime imaging (FLIM) using time-correlated single-photon counting ...
Jakub Nedbal, Francesco Mattioli Della Rocca, Iveta T Ivanova, Andrew Allan, J. Graham, Richard Walker, R. Henderson, K. Suhling   +7 more
semanticscholar   +1 more source

Wide-Field Multi-Parameter FLIM: long-term minimal invasive observation of proteins in living cells. [PDF]

PLoS ONE, 2011
Time-domain Fluorescence Lifetime Imaging Microscopy (FLIM) is a remarkable tool to monitor the dynamics of fluorophore-tagged protein domains inside living cells.
Marco Vitali, Fernando Picazo, Yury Prokazov, Alessandro Duci, Evgeny Turbin, Christian Götze, Juan Llopis, Roland Hartig, Antonie J W G Visser, Werner Zuschratter   +9 more
doaj   +1 more source

A 512 × 512 SPAD Image Sensor With Integrated Gating for Widefield FLIM

IEEE Journal of Selected Topics in Quantum Electronics, 2019
In this paper, we report on SwissSPAD2, an image sensor with 512 × 512 photon-counting pixels, each comprising a single-photon avalanche diode (SPAD), a 1-b memory, and a gating mechanism capable of turning the SPAD on and off, with a skew of 250 and 344 
A. Ulku, C. Bruschini, I. Antolović, Y. Kuo, R. Ankri, S. Weiss, X. Michalet, E. Charbon   +7 more
semanticscholar   +1 more source

Two-photon-excited fluorescence (TPEF) and fluorescence lifetime imaging (FLIM) with sub-nanosecond pulses and a high analog bandwidth signal detection

, 2017
Two-photon excited fluorescence (TPEF) microscopy and fluorescence lifetime imaging (FLIM) are powerful imaging techniques in bio-molecular science. The need for elaborate light sources for TPEF and speed limitations for FLIM, however, hinder an even ...
Eibl, Matthias, Hakert, Hubertus, Huber, Robert, Karpf, Sebastian, Weng, Daniel   +4 more
core   +1 more source

Fluorescence lifetime imaging of fluorescent proteins as an effective quantitative tool for noninvasive study of intracellular processes [PDF]

Journal of Innovative Optical Health Sciences, 2018
Fluorescence lifetime imaging (FLIM) is an effective noninvasive bioanalytical tool based on measuring fluorescent lifetime of fluorophores. A growing number of FLIM studies utilizes genetically engineered fluorescent proteins targeted to specific ...
Svitlana M. Levchenko, Artem Pliss, Junle Qu   +2 more
doaj   +1 more source

Quantitative Imaging of Protein-Protein Interactions by Multiphoton Fluorescence Lifetime Imaging Microscopy using a Streak camera

, 2003
Fluorescence Lifetime Imaging Microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology.
A. Masuda, B. Herman, Becker, Clayton, Clegg, Cole, Elangovan, French, Gadella, Gerritsen, Harpur, Heya, Konig, Konig, Krishnan, Krishnan, Lakowicz, Mahajan, Neuberth, Oida, Periasamy, R. V. Krishnan, Schonle, Selvin, Squire, Squire, Straub, Sytsma, Szmacinski, Tahara, Tavare, V. E. Centonze, van Zandvoort, Verveer, Verveer, Verveer   +35 more
core   +1 more source

FLIM-FRET for Cancer Applications [PDF]

Current Molecular Imaging, 2015
Optical imaging assays, especially fluorescence molecular assays, are minimally invasive if not completely noninvasive, and thus an ideal technique to be applied to live specimens. These fluorescence imaging assays are a powerful tool in biomedical sciences as they allow the study of a wide range of molecular and physiological events occurring in ...
Shilpi, Rajoria, Lingling, Zhao, Xavier, Intes, Margarida, Barroso   +3 more
openaire   +2 more sources

Impact of Coenzyme Q10 on Mitochondrial Metabolism: A Complementary Study Using Fluorescence Lifetime Imaging and Electron Microscopy

Frontiers in Bioscience-Landmark
Background: Coenzyme Q10 (CoQ10), also known as ubiquinone-10, is an important molecule of the mitochondrial respiratory chain that acts as an electron carrier between complexes I, II, and III and additionally functions as an ...
Johannes Georg Wieland, Nilanjon Naskar, Kirsten Reess, Daniela Nobre Sarmento dos Santos, Julia M. Weise, Thomas Blatt, Sebastian Kordes, Paul Walther, Angelika Rück   +8 more
doaj   +1 more source

Robustness in an Ultrasensitive Motor

mBio, 2020
In Escherichia coli, the chemotaxis response regulator CheY-P binds to FliM, a component of the switch complex at the base of the bacterial flagellar motor, to modulate the direction of motor rotation.
Guangzhe Liu, Antai Tao, Rongjing Zhang, Junhua Yuan   +3 more
doaj   +1 more source

Resolution of 4 components in the same pixel in FLIM images using the phasor approach

Methods and Applications in Fluorescence, 2020
In several cellular systems, the phasor FLIM approach has shown the existence of more than 2 components in the same pixel, a typical example being free and bound NADH.
Alexander Vallmitjana, A. Dvornikov, Belén Torrado, D. Jameson, S. Ranjit, E. Gratton   +5 more
semanticscholar   +1 more source