Results 21 to 30 of about 9,845 (208)

Impact of Coenzyme Q10 on Mitochondrial Metabolism: A Complementary Study Using Fluorescence Lifetime Imaging and Electron Microscopy

Frontiers in Bioscience-Landmark
Background: Coenzyme Q10 (CoQ10), also known as ubiquinone-10, is an important molecule of the mitochondrial respiratory chain that acts as an electron carrier between complexes I, II, and III and additionally functions as an ...
Johannes Georg Wieland, Nilanjon Naskar, Kirsten Reess, Daniela Nobre Sarmento dos Santos, Julia M. Weise, Thomas Blatt, Sebastian Kordes, Paul Walther, Angelika Rück   +8 more
doaj   +1 more source

Wide-Field Multi-Parameter FLIM: long-term minimal invasive observation of proteins in living cells. [PDF]

PLoS ONE, 2011
Time-domain Fluorescence Lifetime Imaging Microscopy (FLIM) is a remarkable tool to monitor the dynamics of fluorophore-tagged protein domains inside living cells.
Marco Vitali, Fernando Picazo, Yury Prokazov, Alessandro Duci, Evgeny Turbin, Christian Götze, Juan Llopis, Roland Hartig, Antonie J W G Visser, Werner Zuschratter   +9 more
doaj   +1 more source

Improving fluorescence lifetime imaging microscopy phasor accuracy using convolutional neural networks

Frontiers in Bioinformatics, 2023
Introduction: Although a powerful biological imaging technique, fluorescence lifetime imaging microscopy (FLIM) faces challenges such as a slow acquisition rate, a low signal-to-noise ratio (SNR), and high cost and complexity.
Varun Mannam, Jacob P. Brandt, Cody J. Smith, Xiaotong Yuan, Scott Howard   +4 more
doaj   +1 more source

Fluorescence lifetime imaging of fluorescent proteins as an effective quantitative tool for noninvasive study of intracellular processes [PDF]

Journal of Innovative Optical Health Sciences, 2018
Fluorescence lifetime imaging (FLIM) is an effective noninvasive bioanalytical tool based on measuring fluorescent lifetime of fluorophores. A growing number of FLIM studies utilizes genetically engineered fluorescent proteins targeted to specific ...
Svitlana M. Levchenko, Artem Pliss, Junle Qu   +2 more
doaj   +1 more source

Flagellar Basal Body Structural Proteins FlhB, FliM, and FliY Are Required for Flagellar-Associated Protein Expression in Listeria monocytogenes

Frontiers in Microbiology, 2018
Listeria monocytogenes is a food-associated bacterium that is responsible for food-related illnesses worldwide. In the L. monocytogenes EGD-e genome, FlhB, FliM, and FliY (encoded by lmo0679, lmo0699, and lmo0700, respectively) are annotated as putative ...
Changyong Cheng, Changyong Cheng, Hang Wang, Tiantian Ma, Xiao Han, Yongchun Yang, Jing Sun, Zhongwei Chen, Huifei Yu, Yi Hang, Fengdan Liu, Weihuan Fang, Weihuan Fang, Lingli Jiang, Chang Cai, Chang Cai, Houhui Song   +16 more
doaj   +1 more source

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells [PDF]

FEBS Open Bio
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Hink M.
europepmc   +2 more sources

Mutant p53R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation

Molecular Oncology, EarlyView.
How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Mei Chee Lim, Alvin Kunyao Guo, Yoko Itahana, Srinivasaraghavan Kannan, Valerie Goh, Mingmin Ong, Terina Yan Wen Tay, Weiying Ye, Sabhashina Radha Krishnan, Jun Nishiyama, Chandra S Verma, Koji Itahana   +11 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Robustness in an Ultrasensitive Motor

mBio, 2020
In Escherichia coli, the chemotaxis response regulator CheY-P binds to FliM, a component of the switch complex at the base of the bacterial flagellar motor, to modulate the direction of motor rotation.
Guangzhe Liu, Antai Tao, Rongjing Zhang, Junhua Yuan   +3 more
doaj   +1 more source

Visualising varnish removal for conservation of paintings by fluorescence lifetime imaging (FLIM)

Heritage Science, 2023
The removal of varnish from the surface is a key step in painting conservation. Varnish removal is traditionally monitored by examining the painting surface under ultraviolet illumination.
Christine B. Wilda, Aviva Burnstock, Klaus Suhling, Francesco Mattioli Della Rocca, Robert K. Henderson, Jakub Nedbal   +5 more
doaj   +1 more source