FLIM of NAD(P)H in Lymphatic Nodes Resolves T-Cell Immune Response to the Tumor
International Journal of Molecular Sciences, 2022 Assessment of T-cell response to the tumor is important for diagnosis of the disease and monitoring of therapeutic efficacy. For this, new non-destructive label-free methods are required.
A. Izosimova +7 more
semanticscholar +1 more source
eLife, 2022 Macrophages (ΜΦs) are important immune effector cells that promote (M1 ΜΦs) or inhibit (M2 ΜΦs) inflammation and are involved in numerous physiological and pathogenic immune responses.
M. Kröger +7 more
semanticscholar +1 more source
Dissecting aggregation and seeding dynamics of α-Syn polymorphs using the phasor approach to FLIM
bioRxiv, 2022 Synucleinopathies are a heterogenous group of neurodegenerative diseases characterized by the progressive accumulation of pathological α-synuclein (α-Syn).
Jessica Tittelmeier +4 more
semanticscholar +1 more source
Phasor identifier: A cloud-based analysis of phasor-FLIM data on Python notebooks
Biophysical Reports, 2023 This paper introduces an innovative approach utilizing Google Colaboratory for the versatile analysis of phasor fluorescence lifetime imaging microscopy (FLIM) data collected from various samples (e.g., cuvette, cells, tissues) and in various input file ...
Mario Bernardi, Francesco Cardarelli
doaj +1 more source
Quantifying nuclear wide chromatin compaction by phasor analysis of histone Förster resonance energy transfer (FRET) in frequency domain fluorescence lifetime imaging microscopy (FLIM) data. [PDF]
, 2020 The nanometer spacing between nucleosomes throughout global chromatin organisation modulates local DNA template access, and through continuous dynamic rearrangements, regulates genome function [1].
Gratton, Enrico +4 more
core +3 more sources
Compressed fluorescence lifetime imaging via combined TV-based and deep priors.
PLoS ONE, 2022 Compressed fluorescence lifetime imaging (Compressed-FLIM) is a novel Snapshot compressive imaging (SCI) method for single-shot widefield FLIM. This approach has the advantages of high temporal resolution and deep frame sequences, allowing for the ...
Chao Ji +8 more
doaj +1 more source
Metabolic Imaging by Simultaneous FLIM of NAD(P)H and FAD
Current Directions in Biomedical Engineering, 2020 We describe a metabolic imaging system based on simultaneous recording of lifetime images of NAD(P)H and FAD. The system uses one-photon excitation by ps diode lasers, scanning by galvanometer mirrors, confocal detection, and two parallel TCSPC FLIM ...
Becker Wolfgang +3 more
doaj +1 more source
Phenotypic analysis of extracellular vesicles: a review on the applications of fluorescence
Journal of Extracellular Vesicles, 2020 Extracellular vesicles (EVs) have numerous potential applications in the field of healthcare and diagnostics, and research into their biological functions is rapidly increasing.
Maria S. Panagopoulou +3 more
doaj +1 more source
Instant FLIM enables 4D in vivo lifetime imaging of intact and injured zebrafish and mouse brains.
Optica, 2021 Traditional fluorescence microscopy is blind to molecular microenvironment information that is present in fluorescence lifetime, which can be measured by fluorescence lifetime imaging microscopy (FLIM).
Yide Zhang +6 more
semanticscholar +1 more source
Molecular rheometry: direct determination of viscosity in L-o and L-d lipid phases via fluorescence lifetime imaging [PDF]
, 2013 Understanding of cellular regulatory pathways that involve lipid membranes requires the detailed knowledge of their physical state and structure. However, mapping the viscosity and diffusion in the membranes of complex composition is currently a non ...
Agnieszka Olzyńska +37 more
core +2 more sources