Results 11 to 20 of about 36,842 (272)

In vivo melanin 3D quantification and z-epidermal distribution by multiphoton FLIM, phasor and Pseudo-FLIM analyses

Scientific Reports, 2022
Characterizing melanins in situ and determining their 3D z-epidermal distribution is paramount for understanding physiological/pathological processes of melanin neosynthesis, transfer, degradation or modulation with external UV exposure or cosmetic ...
Ana-Maria Pena, Etienne Decencière, Sébastien Brizion, Peggy Sextius, Serge Koudoro, Thérèse Baldeweck, Emmanuelle Tancrède-Bohin   +6 more
doaj   +2 more sources

Particle-based phasor-FLIM-FRET resolves protein-protein interactions inside single viral particles

Biophysical Reports, 2023
Fluorescence lifetime imaging microscopy (FLIM) is a popular modality to create additional contrast in fluorescence images. By carefully analyzing pixel-based nanosecond lifetime patterns, FLIM allows studying complex molecular populations. At the single-
Quinten Coucke, Nagma Parveen, Guillermo Solís Fernández, Chen Qian, Johan Hofkens, Zeger Debyser, Jelle Hendrix   +6 more
doaj   +2 more sources

Dynamic FRET-FLIM based screening of signal transduction pathways

Scientific Reports, 2021
Fluorescence Lifetime Imaging (FLIM) is an intrinsically quantitative method to screen for protein–protein interactions and is frequently used to record the outcome of signal transduction events.
Rolf Harkes, Olga Kukk, Sravasti Mukherjee, Jeffrey Klarenbeek, Bram van den Broek, Kees Jalink   +5 more
doaj   +2 more sources

Fluorescence Lifetime Imaging Microscopy (FLIM) reveals spatial-metabolic changes in 3D breast cancer spheroids

Scientific Reports, 2023
Cancer cells are mechanically sensitive to physical properties of the microenvironment, which can affect downstream signaling to promote malignancy, in part through the modulation of metabolic pathways. Fluorescence Lifetime Imaging Microscopy (FLIM) can
Kavon Karrobi, Anup Tank, Mohammad Ahsan Fuzail, M. Kalidoss, Karissa B. Tilbury, Muhammad Zaman, Jacopo Ferruzzi, D. Roblyer   +7 more
semanticscholar   +1 more source

FLUTE: A Python GUI for interactive phasor analysis of FLIM data

bioRxiv, 2023
Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique used to probe the local environment of fluorophores. The phasor approach to FLIM data is a fit-free analysis and is increasingly used due to its ease of interpretation.
Dale Gottlieb, Bahar Asadipour, T. P. L. Ung, C. Stringari   +3 more
semanticscholar   +1 more source

A Rapid Method for Detecting Microplastics Based on Fluorescence Lifetime Imaging Technology (FLIM)

Toxics, 2022
With the increasing use and release of plastic products, microplastics have rapidly accumulated in ecological environments. When microplastics enter the food chain, they cause serious harm to organisms and humans.
Fangyu Zhou, Xin Wang, Guangxin Wang, Yanxia Zuo   +3 more
semanticscholar   +1 more source

Characterization of mitochondrial dysfunction due to laser damage by 2-photon FLIM microscopy

Scientific Reports, 2022
Mitochondria are the central organelles in cellular bio-energetics with key roles to play in energy metabolism and cell fate decisions. Fluorescence Lifetime Imaging microscopy (FLIM) is used to track metabolic changes by following the intrinsic co ...
S. R. Alam, Horst Wallrabe, Kathryn G. Christopher, K. Siller, A. Periasamy   +4 more
semanticscholar   +1 more source

Non-invasive classification of macrophage polarisation by 2P-FLIM and machine learning

bioRxiv, 2022
In this study, fluorescence lifetime imaging of NAD(P)H-based cellular autofluorescence is applied as a non-invasive modality to classify two contrasting states of human macrophages by proxy of their governing metabolic state.
N. Neto, Sinead A. O'Rourke, Mimi Zhang, Hannah K. Fitzgerald, A. Dunne, M. Monaghan   +5 more
semanticscholar   +1 more source

Simultaneous Probing of Metabolism and Oxygenation of Tumors In Vivo Using FLIM of NAD(P)H and PLIM of a New Polymeric Ir(III) Oxygen Sensor

International Journal of Molecular Sciences, 2022
Tumor cells are well adapted to grow in conditions of variable oxygen supply and hypoxia by switching between different metabolic pathways. However, the regulatory effect of oxygen on metabolism and its contribution to the metabolic heterogeneity of ...
Yulia P Parshina, Anastasia D Komarova, L. N. Bochkarev, Tatyana A Kovylina, A. Plekhanov, L. Klapshina, A. N. Konev, Artem M. Mozherov, Ilya Shchechkin, M. Sirotkina, V. Shcheslavskiy, M. Shirmanova   +11 more
semanticscholar   +1 more source

Endogenous Labelling of Extracellular Vesicles and Image Capture of Their Interactions With Acceptor Cells. [PDF]

Chembiochem
Isolation and characterisation of endogenously labelled extracellular vesicles (EVs) from donor cells are timelapse imaged using intensity and lifetime microscopies to capture EV contact with filopodia and the plasma membrane (PM) of acceptor cells. Accumulation of EVs within and around filipodia could be observed and following passage through the PM ...
Booth E, Garre M, Wu D, O'Shea DF.
europepmc   +2 more sources