Results 11 to 20 of about 9,845 (208)

Reversible, Chemically Gated FRET via Ligand-Activated Acceptors. [PDF]

Adv Sci (Weinh)
Fluorogen binding turns the compact FAST tag into a tunable FRET acceptor, with ligand chemistry programming spectral overlap and excited‐state lifetimes. Fluorescence‐lifetime imaging reads out energy transfer through the shortened donor lifetime, while simply adding or washing out the dye switches FRET on and off, reversibly mapping the supramodular ...
Singh N, Nayak SR, Mohanty SK, Hanumantharaju A, Shaju A, Jose M, Penmatsa A, Nair D.   +7 more
europepmc   +2 more sources

Carbohydrate-Mediated Cellular Uptake of Boronic Acid Hybrids In Cellulo and Insulin-Deficient Zebrafish: Live Imaging and Application of Multiphoton FLIM. [PDF]

Adv Sci (Weinh)
Boronic acid‐based fluorescent hybrids have been used as part of a versatile platform for real‐time metabolic bioimaging across cellular and organismal scales. Including glucose‐responsive imaging in living cells and in an insulin‐deficient vertebrate model, this work opens new opportunities for the development of precision diagnostics, metabolic ...
Ge H, Fossey JS, Calatayud DG, Flower SE, Wang S, Aitessaid H, Tvardauskaite M, Dudzic J, Jewell M, Pourzand C, Botchway SW, Gurevich D, James TD, Pascu SI.   +13 more
europepmc   +2 more sources

Compressed fluorescence lifetime imaging via combined TV-based and deep priors.

PLoS ONE, 2022
Compressed fluorescence lifetime imaging (Compressed-FLIM) is a novel Snapshot compressive imaging (SCI) method for single-shot widefield FLIM. This approach has the advantages of high temporal resolution and deep frame sequences, allowing for the ...
Chao Ji, Xing Wang, Kai He, Yanhua Xue, Yahui Li, Liwei Xin, Wei Zhao, Jinshou Tian, Liang Sheng   +8 more
doaj   +1 more source

Phenotypic analysis of extracellular vesicles: a review on the applications of fluorescence

Journal of Extracellular Vesicles, 2020
Extracellular vesicles (EVs) have numerous potential applications in the field of healthcare and diagnostics, and research into their biological functions is rapidly increasing.
Maria S. Panagopoulou, Alastair W. Wark, David J S Birch, Christopher D. Gregory   +3 more
doaj   +1 more source

Two-Photon Fluorescence and Second-Harmonic Generation Microscopy: Principles and Biomedical Applications. [PDF]

J Biophotonics
Compared with single‐photon methods, TPM enables deep‐tissue imaging using near‐infrared light, with excitation confined to the focal plane, thereby reducing photodamage. SHG microscopy images noncentrosymmetric biological structures such as collagen and myosin without fluorescent labels, offering direct structural contrast.
Makkithaya KN, Raju G, Das S, Patil A, Kumar R, Kistenev YV, Zhuo GY, Mazumder N.   +7 more
europepmc   +2 more sources

FLIM-FRET for Cancer Applications [PDF]

Current Molecular Imaging, 2015
Optical imaging assays, especially fluorescence molecular assays, are minimally invasive if not completely noninvasive, and thus an ideal technique to be applied to live specimens. These fluorescence imaging assays are a powerful tool in biomedical sciences as they allow the study of a wide range of molecular and physiological events occurring in ...
Shilpi, Rajoria, Lingling, Zhao, Xavier, Intes, Margarida, Barroso   +3 more
openaire   +2 more sources

In vivo melanin 3D quantification and z-epidermal distribution by multiphoton FLIM, phasor and Pseudo-FLIM analyses

Scientific Reports, 2022
Characterizing melanins in situ and determining their 3D z-epidermal distribution is paramount for understanding physiological/pathological processes of melanin neosynthesis, transfer, degradation or modulation with external UV exposure or cosmetic ...
Ana-Maria Pena, Etienne Decencière, Sébastien Brizion, Peggy Sextius, Serge Koudoro, Thérèse Baldeweck, Emmanuelle Tancrède-Bohin   +6 more
doaj   +1 more source

A pH-Responsive fluorescence lifetime probe reveals GOT1 phase separation in cellular anti-senescence. [PDF]

Smart Mol
Acidic pH triggers GOT1 phase separation via IDR1, recruiting ME1 to form ROS‐scavenging condensates that combat senescence, revealed by a FLIM‐based pH probe. Abstract Cellular senescence involves progressive acidification, but how cells sense and adapt to this pH shift remains unclear.
Chen Z, Li J, Bao Y, Wang M, Chen H, Zhang M, Ding L, Wu S, Shen B.   +8 more
europepmc   +2 more sources

FRET Microscopy in Yeast

Biosensors, 2019
Förster resonance energy transfer (FRET) microscopy is a powerful fluorescence microscopy method to study the nanoscale organization of multiprotein assemblies in vivo.
Michal Skruzny, Emma Pohl, Marc Abella
doaj   +1 more source

FLIMJ: An open-source ImageJ toolkit for fluorescence lifetime image data analysis.

PLoS ONE, 2020
In the field of fluorescence microscopy, there is continued demand for dynamic technologies that can exploit the complete information from every pixel of an image.
Dasong Gao, Paul R Barber, Jenu V Chacko, Md Abdul Kader Sagar, Curtis T Rueden, Aivar R Grislis, Mark C Hiner, Kevin W Eliceiri   +7 more
doaj   +1 more source