Results 1 to 10 of about 77,383 (116)

Carbon Dots for Intracellular pH Sensing with Fluorescence Lifetime Imaging Microscopy

Nanomaterials, 2020
The monitoring of intracellular pH is of great importance for understanding intracellular trafficking and functions. It has various limitations for biosensing based on the fluorescence intensity or spectra study.
Maojia Huang, Xinyue Liang, Zixiao Zhang, Jing Wang, Yiyan Fei, Jiong Ma, Songnan Qu, Lan Mi   +7 more
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The promise and peril of comparing fluorescence lifetime in biology revealed by simulations [PDF]

eLife
Signaling dynamics are crucial in biological systems, and biosensor-based real-time imaging has revolutionized their analysis. Fluorescence lifetime imaging microscopy (FLIM) excels over the widely used fluorescence intensity imaging by allowing the ...
Pingchuan Ma, Peter Chen, Scott Sternson, Yao Chen   +3 more
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Chemical Regulation of Fluorescence Lifetime

Chemical & Biomedical Imaging, 2023
Jianan Dai, Xin Zhang
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Imaging mitochondrial membrane potential via concentration-dependent fluorescence lifetime changes [PDF]

Nature Communications
Mitochondria are central to cellular metabolism. Various fluorescence tools have been developed for imaging the mitochondrial environment. Yet, new reporters and imaging methods for directly reading the mitochondrial status are needed for high spatial ...
Dilizhatai Saimi, Luc Reymond, Tursunjan Aziz, Xuan Shen, Ziying Luo, Shuaibo Pi, Yitong Liu, Song Fu, Shuangjin Ding, Anming Meng, Liangyi Chen, Hui Jiang, Zhixing Chen   +12 more
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Fluorescence lifetime Hong-Ou-Mandel sensing

Nature Communications, 2023
Fluorescence Lifetime Imaging Microscopy in the time domain is typically performed by recording the arrival time of photons either by using electronic time tagging or a gated detector.
Ashley Lyons, Vytautas Zickus, Raúl Álvarez-Mendoza, Danilo Triggiani, Vincenzo Tamma, Niclas Westerberg, Manlio Tassieri, Daniele Faccio   +7 more
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Nanosecond-order long–short fluorescence lifetime switchable encryption with enlarged coding capacity

Nanophotonics, 2021
The turn-off fluorescent photoswitches for information encryption are constantly being developed. However, there are no reports about time-switchable (fluorescence lifetime-switchable) encryption to overcome the limitations of tunable encoding numbers in
Luo Teng, Zhao Yihua, Zhou Ting, Qu Junle   +3 more
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PDT-Induced Variations of Radachlorin Fluorescence Lifetime in Living Cells In Vitro

Photonics, 2023
Variations in the fluorescence lifetimes of Radachlorin photosensitizers in HeLa and A549 cells, caused by photodynamic treatment, were studied using fluorescence lifetime imaging microscopy (FLIM).
Andrey V. Belashov, Anna A. Zhikhoreva, Anna V. Salova, Tatiana N. Belyaeva, Ilia K. Litvinov, Elena S. Kornilova, Irina V. Semenova   +6 more
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Applications of fluorescence lifetime imaging in clinical medicine [PDF]

Journal of Innovative Optical Health Sciences, 2018
Fluorescence lifetime is not only associated with the molecular structure of fluorophores, but also strongly depends on the environment around them, which allows fluorescence lifetime imaging microscopy (FLIM) to be used as a tool for precise measurement
Zhanwen Wang, Yanping Zheng, Deqiang Zhao, Ziwei Zhao, Lixin Liu, Artem Pliss, Feiqi Zhu, Jun Liu, Junle Qu, Ping Luan   +9 more
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Advanced Data Analysis for Fluorescence-Lifetime Single-Molecule Localization Microscopy

Frontiers in Bioinformatics, 2021
Fluorescence-lifetime single molecule localization microscopy (FL-SMLM) adds the lifetime dimension to the spatial super-resolution provided by SMLM. Independent of intensity and spectrum, this lifetime information can be used, for example, to quantify ...
Jan Christoph Thiele, Oleksii Nevskyi, Dominic A. Helmerich, Markus Sauer, Jörg Enderlein, Jörg Enderlein   +5 more
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Quantitative Imaging of Genetically Encoded Fluorescence Lifetime Biosensors

Biosensors, 2023
Genetically encoded fluorescence lifetime biosensors have emerged as powerful tools for quantitative imaging, enabling precise measurement of cellular metabolites, molecular interactions, and dynamic cellular processes.
Cong Quang Vu, Satoshi Arai
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