Results 1 to 10 of about 207,530 (298)

Chemical Regulation of Fluorescence Lifetime

Chemical & Biomedical Imaging, 2023
Fluorescence lifetime has significant applications in the field of fluorescence microscopy. Effective modulation of fluorescence lifetime can be achieved by controlling the radiative versus nonradiative processes of fluorophores. In this review, we systematically analyze and summarize chemical approaches that achieve fluorescence lifetime modulation ...
Jianan Dai, Xin Zhang
doaj   +4 more sources

The promise and peril of comparing fluorescence lifetime in biology revealed by simulations [PDF]

eLife
Signaling dynamics are crucial in biological systems, and biosensor-based real-time imaging has revolutionized their analysis. Fluorescence lifetime imaging microscopy (FLIM) excels over the widely used fluorescence intensity imaging by allowing the ...
Pingchuan Ma, Peter Chen, Scott Sternson, Yao Chen   +3 more
doaj   +2 more sources

Carbon Dots for Intracellular pH Sensing with Fluorescence Lifetime Imaging Microscopy

Nanomaterials, 2020
The monitoring of intracellular pH is of great importance for understanding intracellular trafficking and functions. It has various limitations for biosensing based on the fluorescence intensity or spectra study.
Maojia Huang, Xinyue Liang, Zixiao Zhang, Jing Wang, Yiyan Fei, Jiong Ma, Songnan Qu, Lan Mi   +7 more
doaj   +3 more sources

Applications of fluorescence lifetime imaging in clinical medicine [PDF]

Journal of Innovative Optical Health Sciences, 2018
Fluorescence lifetime is not only associated with the molecular structure of fluorophores, but also strongly depends on the environment around them, which allows fluorescence lifetime imaging microscopy (FLIM) to be used as a tool for precise measurement
Zhanwen Wang, Yanping Zheng, Deqiang Zhao, Ziwei Zhao, Lixin Liu, Artem Pliss, Feiqi Zhu, Jun Liu, Junle Qu, Ping Luan   +9 more
doaj   +3 more sources

Imaging mitochondrial membrane potential via concentration-dependent fluorescence lifetime changes [PDF]

Nature Communications
Mitochondria are central to cellular metabolism. Various fluorescence tools have been developed for imaging the mitochondrial environment. Yet, new reporters and imaging methods for directly reading the mitochondrial status are needed for high spatial ...
Dilizhatai Saimi, Luc Reymond, Tursunjan Aziz, Xuan Shen, Ziying Luo, Shuaibo Pi, Yitong Liu, Song Fu, Shuangjin Ding, Anming Meng, Liangyi Chen, Hui Jiang, Zhixing Chen   +12 more
doaj   +2 more sources

Multiplexed In Vivo Imaging with Fluorescence Lifetime‐Modulating Tags

Advanced Science
Fluorescence lifetime imaging microscopy (FLIM) opens new dimensions for highly multiplexed imaging in live cells and organisms using differences in fluorescence lifetime to distinguish spectrally identical fluorescent probes. Here, a set of fluorescence‐
Lina El Hajji, France Lam, Maria Avtodeeva, Hela Benaissa, Christine Rampon, Michel Volovitch, Sophie Vriz, Arnaud Gautier   +7 more
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Real-time wide-field fluorescence lifetime imaging via single-snapshot acquisition for biomedical applications [PDF]

PhotoniX
Fluorescence lifetime imaging (FLI) is a powerful tool for investigating molecular processes, microenvironmental parameters, and molecular interactions across tissue to (sub-)cellular levels.
Vikas Pandey, Euan Millar, Ismail Erbas, Luis Chavez, Jack Radford, Isaiah Crosbourne, Mansa Madhusudan, Gregor G. Taylor, Nanxue Yuan, Claudio Bruschini, Stefan T. Radev, Margarida M. Barroso, Andrew Tobin, Xavier Michalet, Edoardo Charbon, Daniele Faccio, Xavier Intes   +16 more
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Silver Nanoparticle Aggregates as Highly Efficient Plasmonic Antennas for Fluorescence Enhancement [PDF]

, 2012
The enhanced local fields around plasmonic structures can lead to enhancement of the excitation and modification of the emission quantum yield of fluorophores.
Amerongen, H., Gill, R., Ru, E.C. le, Somerville, W.R.C., Subramaniam, V., Tian, L.   +5 more
core   +16 more sources

Fluorescence lifetime Hong-Ou-Mandel sensing

Nature Communications, 2023
Fluorescence Lifetime Imaging Microscopy in the time domain is typically performed by recording the arrival time of photons either by using electronic time tagging or a gated detector.
Ashley Lyons, Vytautas Zickus, Raúl Álvarez-Mendoza, Danilo Triggiani, Vincenzo Tamma, Niclas Westerberg, Manlio Tassieri, Daniele Faccio   +7 more
doaj   +1 more source

Fluorescence lifetime imaging [PDF]

Analytical Biochemistry, 1992
We describe a new fluorescence imaging methodology in which the image contrast is derived from the fluorescence lifetime at each point in a two-dimensional image and not the local concentration and/or intensity of the fluorophore. In the present apparatus, lifetime images are created from a series of images obtained with a gain-modulated image ...
J R, Lakowicz, H, Szmacinski, K, Nowaczyk, K W, Berndt, M, Johnson   +4 more
openaire   +2 more sources