Results 41 to 50 of about 207,530 (298)
Widefield multifrequency fluorescence lifetime imaging using a two-tap complementary metal-oxide semiconductor camera with lateral electric field charge modulators. [PDF]
, 2019 Widefield frequency-domain fluorescence lifetime imaging microscopy (FD-FLIM) measures the fluorescence lifetime of entire images in a fast and efficient manner.
Chen, Hongtao +5 more
core
Optical Maser Emission from Trivalent Praseodymium in Calcium Tungstate [PDF]
, 1962 Coherent emission at 1.047 µ from trivalent praseodymium in calcium tungstate was observed. This emission coincides with strong infrared fluorescence at the same wavelength and was found to be stimulated mostly by absorption of blue light by the 3P0, 3P1,
Nassau, K., Porto, S. P. S., Yariv, A.
core +1 more source
Tau acetylation at K331 has limited impact on tau pathology in vivo
FEBS Letters, EarlyView.We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto +3 more
wiley +1 more source
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Characterization of SPAD Array for Multifocal High-Content Screening Applications
Photonics, 2016 Current instruments used to detect specific protein-protein interactions in live cells for applications in high-content screening (HCS) are limited by the time required to measure the lifetime.
Anthony Tsikouras +5 more
doaj +1 more source
Molecular rheometry: direct determination of viscosity in L-o and L-d lipid phases via fluorescence lifetime imaging [PDF]
, 2013 Understanding of cellular regulatory pathways that involve lipid membranes requires the detailed knowledge of their physical state and structure. However, mapping the viscosity and diffusion in the membranes of complex composition is currently a non ...
Agnieszka Olzyńska +37 more
core +2 more sources
Stroboscopic fluorescence lifetime imaging
Optics Express, 2009 We report a fluorescence lifetime imaging technique that uses the time integrated response to a periodic optical excitation, eliminating the need for time resolution in detection. A Dirac pulse train of variable period is used to probe the frequency response of the total fluorescence per pulse leading to a frequency roll-off that is dependent on the ...
Holton, Mark D. +6 more
openaire +4 more sources
Molecular Oncology, EarlyView.A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel +12 more
wiley +1 more source
Molecules, 2015 Interactions of nanoparticles with biomaterials determine the biological activity that is key for the physiological response. Dendritic polyglycerol sulfates (dPGS) were found recently to act as an inhibitor of inflammation by blocking selectins ...
Alexander Boreham +8 more
doaj +1 more source
Ultrafast spectroscopy of single molecules [PDF]
, 2007 We present a single-molecule study on femtosecond dynamics in multichromophoric systems, combining fs pump-probe, emission-spectra and fluorescence-lifetime analysis.
E.M.H.P. Dijk van +6 more
core +3 more sources