Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy: Concepts and Applications
We review the basic concepts and recent applications of two-dimensional fluorescence lifetime correlation spectroscopy (2D FLCS), which is the extension of fluorescence correlation spectroscopy (FCS) to analyze the correlation of fluorescence lifetime in
Takuhiro Otosu, Shoichi Yamaguchi
doaj +1 more source
Fluorescence lifetime imaging ophthalmoscopy
Imaging techniques based on retinal autofluorescence have found broad applications in ophthalmology because they are extremely sensitive and noninvasive. Conventional fundus autofluorescence imaging measures fluorescence intensity of endogenous retinal fluorophores.
Chantal Dysli +5 more
openaire +4 more sources
Novel application of fluorescence lifetime and fluorescence microscopy enables quantitative access to subcellular dynamics in plant cells. [PDF]
BACKGROUND: Optical and spectroscopic technologies working at subcellular resolution with quantitative output are required for a deeper understanding of molecular processes and mechanisms in living cells.
Kirstin Elgass +5 more
doaj +1 more source
Single- and multi-photon excited fluorescence from serotonin complexed with B-cyclodextrin [PDF]
The fluorescence of serotonin on binding with B-cyclodextrin has been studied using both steady-state and time-resolved methods. Steady state fluorescence intensity of serotonin at 340 nm showed ~ 30% increase in intensity on binding with Ka ~ 60 dm3 mol
Anthony W. Parker +27 more
core +1 more source
Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Abbe +295 more
core +2 more sources
By utilizing spatially resolved fluorescence (-lifetime) measurements and high precision X-ray nanodiffraction, the authors correlate the influence of structural misalignment and fluorescence (-lifetime) properties of all-inorganic CsPbX3 (X– = Br–, Cl–)
Dmitry Lapkin +18 more
doaj +1 more source
Characterization of the aggregation-induced enhanced emission of N,N'-bis(4-methoxysalicylide)benzene-1,4-diamine [PDF]
© 2015 Springer Science+Business Media New York. N,N′-bis(4-methoxysalicylide)benzene-1,4-diamine (S1) was synthesized from 4-methoxysalicylaldehyde and p-phenylenediamine and it was found to exhibit interesting aggregation-induced emission enhancement ...
Mu, Lan +5 more
core +1 more source
Aggregation-induced emission luminogen for in vivo three-photon fluorescence lifetime microscopic imaging [PDF]
Compared with visible light, near-infrared (NIR) light has deeper penetration in biological tissues. Three-photon fluorescence microscopy (3PFM) can effectively utilize the NIR excitation to obtain high-contrast images in the deep tissue.
Huwei Ni +5 more
doaj +1 more source
Precise intraoperative brain tumor visualization supports surgeons in achieving maximal safe resection. In this sense, improved prognosis in patients with high-grade gliomas undergoing protoporphyrin IX fluorescence-guided surgery has been demonstrated ...
David Reichert +14 more
doaj +1 more source
Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy
Steady-state fluorence imaging and time-resolved fluorescence imaging are two important areas in fluorescence imaging research. Fluorescence lifetime imaging is an absolute measurement method which is independent of excitation laser intensity ...
Chao Liu +3 more
doaj +1 more source

