Results 31 to 40 of about 77,532 (261)

Multiplexed In Vivo Imaging with Fluorescence Lifetime‐Modulating Tags

Advanced Science
Fluorescence lifetime imaging microscopy (FLIM) opens new dimensions for highly multiplexed imaging in live cells and organisms using differences in fluorescence lifetime to distinguish spectrally identical fluorescent probes. Here, a set of fluorescence‐
Lina El Hajji, France Lam, Maria Avtodeeva, Hela Benaissa, Christine Rampon, Michel Volovitch, Sophie Vriz, Arnaud Gautier   +7 more
doaj   +1 more source

Characterization of SPAD Array for Multifocal High-Content Screening Applications

Photonics, 2016
Current instruments used to detect specific protein-protein interactions in live cells for applications in high-content screening (HCS) are limited by the time required to measure the lifetime.
Anthony Tsikouras, Pietro Peronio, Ivan Rech, Nehad Hirmiz, M. Jamal Deen, Qiyin Fang   +5 more
doaj   +1 more source

Aggregation-induced emission luminogen for in vivo three-photon fluorescence lifetime microscopic imaging [PDF]

Journal of Innovative Optical Health Sciences, 2019
Compared with visible light, near-infrared (NIR) light has deeper penetration in biological tissues. Three-photon fluorescence microscopy (3PFM) can effectively utilize the NIR excitation to obtain high-contrast images in the deep tissue.
Huwei Ni, Zicong Xu, Dongyu Li, Ming Chen, Ben Zhong Tang, Jun Qian   +5 more
doaj   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

Fluorescence lifetime in cardiovascular diagnostics [PDF]

Journal of Biomedical Optics, 2010
We review fluorescence lifetime techniques including time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) and fluorescence lifetime imaging microscopy (FLIM) instrumentation and associated methodologies that allow for characterization and diagnosis of atherosclerotic plaques.
openaire   +2 more sources

Fluorescence lifetime probe of biomolecular conformations [PDF]

Journal of the American Society for Mass Spectrometry, 2010
Methods have been developed to measure the fluorescence lifetime versus temperature of trapped biomolecular ions derivatized with a fluorescent dye. Previous measurements for different sequences of polyproline peptides demonstrated that quenching rates are related to conformations and their spatial fluctuations.
Shi, Xiangguo, Parks, Joel H.
openaire   +2 more sources

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Detecting and Quantifying Biomolecular Interactions of a Dendritic Polyglycerol Sulfate Nanoparticle Using Fluorescence Lifetime Measurements

Molecules, 2015
Interactions of nanoparticles with biomaterials determine the biological activity that is key for the physiological response. Dendritic polyglycerol sulfates (dPGS) were found recently to act as an inhibitor of inflammation by blocking selectins ...
Alexander Boreham, Jens Pikkemaat, Pierre Volz, Robert Brodwolf, Christian Kuehne, Kai Licha, Rainer Haag, Jens Dernedde, Ulrike Alexiev   +8 more
doaj   +1 more source

Invisible but not inaccessible—Revealing transient oligomers formed by intrinsically disordered proteins with solution NMR and complementary methods

FEBS Letters, EarlyView.
Transient oligomers formed by intrinsically disordered proteins may be ‘invisible’ to direct detection yet remain accessible to solution NMR through equilibrium‐exchange measurements and pressure‐jump experiments. Complementary methods report on mass, stoichiometry, selected distance distributions, morphology, and internal packing.
Martin D. Gelenter, Ad Bax
wiley   +1 more source

Rapid fluorescence lifetime imaging microscopy via few-photon imaging

APL Photonics
Conventional fluorescence lifetime imaging microscopy (FLIM) based on time-correlated single photon counting has great potential in various domains, notably in cellular biology, enabling comprehensive studies encompassing spatiotemporal dynamics and ...
Ming-Jie Sun, Yi-Cheng Zhang, Fang-Rui Lin, Shuai Wang, Li-Wei Liu, Jun-Le Qu   +5 more
doaj   +1 more source