Results 1 to 10 of about 30,156 (112)

Pinhole engineering based enhanced resolution (PEER) for fluorescence lifetime imaging microscopy [PDF]

Communications Biology
In this article, we present a differential confocal microscopy technique based on pinhole engineering that significantly enhances both lateral resolution and axial sectioning depth.
Wonsang Hwang, Sinyoung Jeong, J. Matthew Dubach, Conor L. Evans, Iván Coto Hernández   +4 more
doaj   +2 more sources

Interferometric excitation fluorescence lifetime imaging microscopy [PDF]

Nature Communications
Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique with numerous imaging applications. Yet, one of the limitations of FLIM is that it only provides information about the emitting state.
Pavel Malý, Dita Strachotová, Aleš Holoubek, Petr Heřman   +3 more
doaj   +2 more sources

Improving fluorescence lifetime imaging microscopy phasor accuracy using convolutional neural networks [PDF]

Frontiers in Bioinformatics, 2023
Introduction: Although a powerful biological imaging technique, fluorescence lifetime imaging microscopy (FLIM) faces challenges such as a slow acquisition rate, a low signal-to-noise ratio (SNR), and high cost and complexity.
Varun Mannam, Jacob P. Brandt, Cody J. Smith, Xiaotong Yuan, Scott Howard   +4 more
doaj   +2 more sources

Metabolic profiling of steatotic liver disease by fluorescence lifetime imaging microscopy [PDF]

Communications Medicine
Background Metabolic dysfunction-associated steatotic liver disease is defined by hepatic lipid overload resulting in a metabolic shift and subsequent mitochondrial impairment. Diagnosis currently relies on tissue biopsy and non-invasive tests.
Kaitlyn Purdie, Narain Karedla, Thea Guy, Anna V. Schepers, Ana Isabel Espirito Santo, Huw Colin-York, Kseniya Korobchevskaya, Helena Coker, Carl Lee, Alex Gordon-Weeks, Jagdeep Nanchahal, Marco Fritzsche   +11 more
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Carbon Dots for Intracellular pH Sensing with Fluorescence Lifetime Imaging Microscopy

Nanomaterials, 2020
The monitoring of intracellular pH is of great importance for understanding intracellular trafficking and functions. It has various limitations for biosensing based on the fluorescence intensity or spectra study.
Maojia Huang, Xinyue Liang, Zixiao Zhang, Jing Wang, Yiyan Fei, Jiong Ma, Songnan Qu, Lan Mi   +7 more
doaj   +3 more sources

Label-free metabolic imaging of Pseudomonas aeruginosa infection using two-photon fluorescence lifetime imaging microscopy [PDF]

Frontiers in Cellular and Infection Microbiology
In this study we have applied high-spatial and temporal label-free imaging of individual live multidrug-resistant bacteria and bacteria-infected cells and animal tissue using two-photon fluorescence lifetime imaging microscopy (2p-FLIM).
Greg A. Snyder, Greg A. Snyder, Greg A. Snyder, Tialfi Bergamin de Castro, Alison J. Scott, Alison J. Scott, Krishanu Ray, Krishanu Ray, Krishanu Ray   +8 more
doaj   +2 more sources

Applications of fluorescence lifetime imaging in clinical medicine [PDF]

Journal of Innovative Optical Health Sciences, 2018
Fluorescence lifetime is not only associated with the molecular structure of fluorophores, but also strongly depends on the environment around them, which allows fluorescence lifetime imaging microscopy (FLIM) to be used as a tool for precise measurement
Zhanwen Wang, Yanping Zheng, Deqiang Zhao, Ziwei Zhao, Lixin Liu, Artem Pliss, Feiqi Zhu, Jun Liu, Junle Qu, Ping Luan   +9 more
doaj   +1 more source

Hyperdimensional Imaging Contrast Using an Optical Fiber

Sensors, 2021
Fluorescence properties of a molecule can be used to study the structural and functional nature of biological processes. Physical properties, including fluorescence lifetime, emission spectrum, emission polarization, and others, help researchers probe a ...
Jenu V. Chacko, Han Nim Lee, Wenxin Wu, Marisa S. Otegui, Kevin W. Eliceiri   +4 more
doaj   +1 more source

Light-field tomographic fluorescence lifetime imaging microscopy. [PDF]

Proc Natl Acad Sci U S A
Ma Y, Park J, Huang L, Sen C, Burri S, Bruschini C, Yang X, Cui Q, Cameron RB, Fishbein GA, Gomperts BN, Ozcan A, Charbon E, Gao L.   +13 more
europepmc   +2 more sources

Quantitative Imaging of Genetically Encoded Fluorescence Lifetime Biosensors

Biosensors, 2023
Genetically encoded fluorescence lifetime biosensors have emerged as powerful tools for quantitative imaging, enabling precise measurement of cellular metabolites, molecular interactions, and dynamic cellular processes.
Cong Quang Vu, Satoshi Arai
doaj   +1 more source