Results 1 to 10 of about 79,470 (290)

Interferometric excitation fluorescence lifetime imaging microscopy [PDF]

Nature Communications
Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique with numerous imaging applications. Yet, one of the limitations of FLIM is that it only provides information about the emitting state.
Pavel Malý, Dita Strachotová, Aleš Holoubek, Petr Heřman   +3 more
doaj   +4 more sources

Light-field tomographic fluorescence lifetime imaging microscopy. [PDF]

Proc Natl Acad Sci U S A
Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging technique that enables the visualization of biological samples at the molecular level by measuring the fluorescence decay rate of fluorescent probes. This provides critical information about molecular interactions, environmental changes, and localization within biological systems ...
Ma Y, Park J, Huang L, Sen C, Burri S, Bruschini C, Yang X, Cui Q, Cameron RB, Fishbein GA, Gomperts BN, Ozcan A, Charbon E, Gao L.   +13 more
europepmc   +5 more sources

Fluorescence Lifetime Imaging Microscopy (FLIM) Data Analysis with TIMP [PDF]

Journal of Statistical Software, 2007
Fluorescence Lifetime Imaging Microscopy (FLIM) allows fluorescence lifetime images of biological objects to be collected at 250 nm spatial resolution and at (sub-)nanosecond temporal resolution.
Sergey Laptenok, Katharine M. Mullen, Jan Willem Borst, Ivo H. M. van Stokkum, Vladimir V. Apanasovich, Antonie J. W. G. Visser   +5 more
doaj   +11 more sources

Fluorescence lifetime imaging microscopy [PDF]

Nature Reviews Methods Primers
Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique offering profound insights into a broad spectrum of biological processes such as metabolic imaging, protein–protein interactions and live-cell intracellular dynamics. The future of FLIM appears promising, with continuous technological advancements for time-resolved measurements ...
Belén Torrado, Michelle
exaly   +4 more sources

Improving fluorescence lifetime imaging microscopy phasor accuracy using convolutional neural networks [PDF]

Frontiers in Bioinformatics, 2023
Introduction: Although a powerful biological imaging technique, fluorescence lifetime imaging microscopy (FLIM) faces challenges such as a slow acquisition rate, a low signal-to-noise ratio (SNR), and high cost and complexity.
Varun Mannam, Jacob P. Brandt, Cody J. Smith, Xiaotong Yuan, Scott Howard   +4 more
doaj   +2 more sources

Fluorescence Lifetime Imaging Microscopy of Biomolecular Condensates. [PDF]

Methods Mol Biol, 2023
Biomolecular condensates of ribonucleoproteins (RNPs) such as the transactivation response element (TAR) DNA-binding protein 43 (TDP-43) arise from liquid-liquid phase separation (LLPS) and play vital roles in various biological processes including the formation-dissolution of stress granules (SGs).
Quan MD, Liao SJ, Ferreon JC, Ferreon ACM.   +3 more
europepmc   +3 more sources

Carbon Dots for Intracellular pH Sensing with Fluorescence Lifetime Imaging Microscopy

Nanomaterials, 2020
The monitoring of intracellular pH is of great importance for understanding intracellular trafficking and functions. It has various limitations for biosensing based on the fluorescence intensity or spectra study.
Maojia Huang, Xinyue Liang, Zixiao Zhang, Jing Wang, Yiyan Fei, Jiong Ma, Songnan Qu, Lan Mi   +7 more
doaj   +3 more sources

Label-free metabolic imaging of Pseudomonas aeruginosa infection using two-photon fluorescence lifetime imaging microscopy [PDF]

Frontiers in Cellular and Infection Microbiology
In this study we have applied high-spatial and temporal label-free imaging of individual live multidrug-resistant bacteria and bacteria-infected cells and animal tissue using two-photon fluorescence lifetime imaging microscopy (2p-FLIM).
Greg A. Snyder, Greg A. Snyder, Greg A. Snyder, Tialfi Bergamin de Castro, Alison J. Scott, Alison J. Scott, Krishanu Ray, Krishanu Ray, Krishanu Ray   +8 more
doaj   +2 more sources

Rapid fluorescence lifetime imaging microscopy via few-photon imaging

APL Photonics
Conventional fluorescence lifetime imaging microscopy (FLIM) based on time-correlated single photon counting has great potential in various domains, notably in cellular biology, enabling comprehensive studies encompassing spatiotemporal dynamics and ...
Ming-Jie Sun, Yi-Cheng Zhang, Fang-Rui Lin, Shuai Wang, Li-Wei Liu, Jun-Le Qu   +5 more
doaj   +2 more sources

Advancements in fluorescence lifetime imaging microscopy Instrumentation: Towards high speed and 3D. [PDF]

Curr Opin Solid State Mater Sci
Park J, Gao L.
europepmc   +2 more sources