Results 41 to 50 of about 30,206 (151)
Multiplexed In Vivo Imaging with Fluorescence Lifetime‐Modulating Tags
Advanced ScienceFluorescence lifetime imaging microscopy (FLIM) opens new dimensions for highly multiplexed imaging in live cells and organisms using differences in fluorescence lifetime to distinguish spectrally identical fluorescent probes. Here, a set of fluorescence‐
Lina El Hajji +7 more
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Characterization of yeast strains by fluorescence lifetime imaging microscopy [PDF]
FEMS Yeast Research, 2008 The results of fluorescence lifetime imaging microscopy of selected yeast strains were presented and the fact that the lifetime distributions can be successfully used for strain characterization and differentiation was demonstrated. Four strains of industrially relevant yeast Saccharomyces were excited at 405 nm and the autofluorescence observed within
Hemant, Bhatta, Ewa M, Goldys
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Fluorescence lifetime imaging and electron microscopy: a correlative approach
Histochemistry and Cell Biology, 2022 AbstractFluorescence lifetime imaging microscopy (FLIM) allows the characterization of cellular metabolism by quantifying the rate of free and unbound nicotinamide adenine dinucleotide hydrogen (NADH). This study delineates the correlative imaging of cells with FLIM and electron microscopy (EM).
Johannes G. Wieland +3 more
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The promise and peril of comparing fluorescence lifetime in biology revealed by simulations
eLifeSignaling dynamics are crucial in biological systems, and biosensor-based real-time imaging has revolutionized their analysis. Fluorescence lifetime imaging microscopy (FLIM) excels over the widely used fluorescence intensity imaging by allowing the ...
Pingchuan Ma +3 more
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Scientific Reports, 2021 Fluoro-Gold is a fluorescent neuronal tracer suitable for targeted deep imaging of the nervous system. Widefield fluorescence microscopy enables visualization of Fluoro-Gold, but lacks depth discrimination.
Matthew Q. Miller +4 more
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Dissecting aggregation and seeding dynamics of α-Syn polymorphs using the phasor approach to FLIM
Communications Biology, 2022 Fluorescence lifetime imaging microscopy combined with phasor analysis resolves seeding and aggregation dynamics of different α-synuclein polymorphs in a biosensor cell line.
Jessica Tittelmeier +4 more
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Fluorescence lifetime imaging microscopy for the characterization of atherosclerotic plaques [PDF]
SPIE Proceedings, 2009 Atherosclerotic plaque composition has been associated with plaque instability and rupture. This study investigates the use of fluorescence lifetime imaging microscopy (FLIM) for mapping plaque composition and assessing features of vulnerability.
Jennifer, Phipps +4 more
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Fluorescence Lifetime Imaging Microscopy (FLIM) Data Analysis with TIMP
Journal of Statistical Software, 2007 Fluorescence Lifetime Imaging Microscopy (FLIM) allows fluorescence lifetime images of biological objects to be collected at 250 nm spatial resolution and at (sub-)nanosecond temporal resolution.
Sergey Laptenok +5 more
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Fluorescence lifetime imaging for material studies [PDF]
EPJ Web of ConferencesFluorescence lifetime imaging microscopy (FLIM) is investigated as a contrast mechanism for the characterization of heterogeneous luminescent materials. A Ce:YAG glass-ceramic sample is used as a model system under epi-fluorescence excitation with a 450 ...
Zhang Zeyu +2 more
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Multiphoton Laser Microscopy and Fluorescence Lifetime Imaging for the Evaluation of the Skin
Dermatology Research and Practice, 2012 Multiphoton laser microscopy is a new, non-invasive technique providing access to the skin at a cellular and subcellular level, which is based both on autofluorescence and fluorescence lifetime imaging. Whereas the former considers fluorescence intensity
Stefania Seidenari +9 more
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