Results 51 to 60 of about 79,470 (290)

Image restoration for fluorescence lifetime imaging microscopy (FLIM)

Optics Express, 2008
Computational image restoration finds wide applicability for fluorescence intensity imaging. Relatively little work in this regard has been performed on FLIM images, which also suffer from diminished spatial resolution. In this work, we report two separate approaches to enhance FLIM image quality while maintaining lifetime accuracy.
Dhruv, Sud, Mary-Ann, Mycek
openaire   +2 more sources

One size does not fit all: An in vitro evaluation of the effects of bezafibrate and medroxyprogesterone acetate on human SH‐SY5Y and U‐87 MG cancer cells

FEBS Open Bio, EarlyView.
Drugs previously repurposed to target blood cancers reduced neuroblastoma and glioblastoma cell growth and viability. However, their levels of anticancer activity were different and their clinical application may be problematic due to side effects at effective doses.
Abhishek Kharawatkar, Francesco Michelangeli, Farhat Latif Khanim, Christopher Martin Bunce, William Eustace Basil Johnson   +4 more
wiley   +1 more source

Rapid global fitting of large fluorescence lifetime imaging microscopy datasets.

PLoS ONE, 2013
Fluorescence lifetime imaging (FLIM) is widely applied to obtain quantitative information from fluorescence signals, particularly using Förster Resonant Energy Transfer (FRET) measurements to map, for example, protein-protein interactions.
Sean C Warren, Anca Margineanu, Dominic Alibhai, Douglas J Kelly, Clifford Talbot, Yuriy Alexandrov, Ian Munro, Matilda Katan, Chris Dunsby, Paul M W French   +9 more
doaj   +1 more source

Widefield multifrequency fluorescence lifetime imaging using a two-tap complementary metal-oxide semiconductor camera with lateral electric field charge modulators. [PDF]

, 2019
Widefield frequency-domain fluorescence lifetime imaging microscopy (FD-FLIM) measures the fluorescence lifetime of entire images in a fast and efficient manner.
Chen, Hongtao, Digman, Michelle, Gratton, Enrico, Kagawa, Keiichiro, Kawahito, Shoji, Ma, Ning   +5 more
core  

A surface plasmon enhanced FLIM-FRET imaging approach based on Au nanoparticles [PDF]

, 2017
In this report we have demonstrated a fluorescence resonant energy transfer (FRET)-fluorescence lifetime imaging microscopy (FLIM) combined approach to study the intracellular pathway of gold nanoparticles.
Birch, David JS, Chen, Yu, Yu, Jun, Zhang, Yinan   +3 more
core   +1 more source

Light-field tomographic fluorescence lifetime imaging microscopy

, 2023
Abstract Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging technique that enables the visualization of biological samples at the molecular level by measuring the fluorescence decay rate of fluorescent probes. This provides critical information about molecular interactions, environmental changes, and localization within ...
Liang Gao, Yayao Ma, Luzhe Huang, Chandani Sen, Samuel Burri, Claudio Bruschini, Xilin Yang, Robert Cameron, Gregory Fishbein, Brigitte Gomperts, Aydogan Ozcan, Edoardo Charbon   +11 more
openaire   +2 more sources

Covalent Organic Frameworks for Photocatalytic CO2 Reduction: Metal Integration Principles, Strategies and Functions

Advanced Functional Materials, EarlyView.
Covalent organic frameworks (COFs) with metals have been recognized as versatile platforms for photocatalytic CO2 reduction (CO2PRR). Herein, an overview of metal integration strategies for COFs is systematically summarized. Regulatory mechanisms and structure–activity relationships between metal integration and COF‐based CO2PRR are emphasized.
Jie He, Minxian Zhang, Jinqiang Zhang, Hongqi Sun, Shaobin Wang, Xiaoguang Duan   +5 more
wiley   +1 more source

Cellular trafficking of Sn-2 phosphatidylcholine prodrugs studied with fluorescence lifetime imaging and super-resolution microscopy [PDF]

, 2018
While ...
Achilefu, Samuel, Cui, Grace, Lanza, Gregory M, Lew, Matthew D, Lu, Jin, Maji, Dolonchampa, Pan, Dipanjan, Sarder, Pinaki, Schmieder, Anne H, Yang, Xiaoxia   +9 more
core   +1 more source

Nanothermometry in Living Cells: Physical Limits, Conceptual and Material Challenges

Advanced Functional Materials, EarlyView.
Heat and temperature are fundamental to life. When nanothermometers began probing regions as small as a living cell, they triggered controversial claims of large intracellular temperature gradients. We review physical constraints energy‐conservation, entropy production, thermodynamic fluctuations, and molecular dynamics.
Taras Plakhotnik
wiley   +1 more source

Nanodiamond arrays on glass for quantification and fluorescence characterisation [PDF]

, 2017
Quantifying the variation in emission properties of fluorescent nanodiamonds is important for developing their wide-ranging applicability. Directed self-assembly techniques show promise for positioning nanodiamonds precisely enabling such quantification.
Gibson, Brant C., Greentree, Andrew D., Heffernan, Ashleigh H.   +2 more
core   +3 more sources