Results 51 to 60 of about 30,206 (151)

Fluorescence Lifetime Imaging Microscopy of Intracellular Glucose Dynamics [PDF]

Journal of Diabetes Science and Technology, 2012
Background: One of the major hurdles in studying diabetes pathophysiology is the lack of adequate methodology that allows for direct and real-time determination of glucose transport and metabolism in cells and tissues.
Jithesh V, Veetil, Sha, Jin, Kaiming, Ye
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Imaging mitochondrial membrane potential via concentration-dependent fluorescence lifetime changes

Nature Communications
Mitochondria are central to cellular metabolism. Various fluorescence tools have been developed for imaging the mitochondrial environment. Yet, new reporters and imaging methods for directly reading the mitochondrial status are needed for high spatial ...
Dilizhatai Saimi, Luc Reymond, Tursunjan Aziz, Xuan Shen, Ziying Luo, Shuaibo Pi, Yitong Liu, Song Fu, Shuangjin Ding, Anming Meng, Liangyi Chen, Hui Jiang, Zhixing Chen   +12 more
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Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates

Nature Communications, 2020
Two-photon microscopy has been limited by low acquisition rates due to mechanical scanners. Here, the authors employ pulse-modulated, rapidly wavelength-swept lasers and inertia-free beam steering through angular dispersion in order to achieve non-linear
Sebastian Karpf, Carson T. Riche, Dino Di Carlo, Anubhuti Goel, William A. Zeiger, Anand Suresh, Carlos Portera-Cailliau, Bahram Jalali   +7 more
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Luminescent probes for luminescence lifetime sensing and imaging in live cells: a narrative review

Journal of Bio-X Research, 2020
. Luminescence (mostly fluorescence and phosphorescence) probes are a powerful tool widely used in the life sciences research. They can be used, for example, in the quantitative analysis of physiological parameters, visualization of different cell ...
Kaixuan Nie, Xiao Peng, Wei Yan, Jun Song, Junle Qu   +4 more
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QUANTITATIVE CONFOCAL LASER SCANNING MICROSCOPY

Image Analysis and Stereology, 2011
This paper discusses recent advances in confocal laser scanning microscopy (CLSM) for imaging of 3D structure as well as quantitative characterization of biomolecular interactions and diffusion behaviour by means of one- and two-photon excitation.
Merete Krog Raarup, Jens Randel Nyengaard   +1 more
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Multiphoton Microscopy and Fluorescence Lifetime Imaging [PDF]

, 2018
This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr.
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Saturation-compensated measurements for fluorescence lifetime imaging microscopy

Optics Letters, 2016
Fluorophore saturation is the key factor limiting the speed and excitation range of fluorescence lifetime imaging microscopy (FLIM). For example, fluorophore saturation causes incorrect lifetime measurements when using conventional frequency-domain FLIM at high excitation powers.
Yide, Zhang, Genevieve D, Vigil, Lina, Cao, Aamir A, Khan, David, Benirschke, Tahsin, Ahmed, Patrick, Fay, Scott S, Howard   +7 more
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Monitoring Photosensitizer Uptake Using Two Photon Fluorescence Lifetime Imaging Microscopy

Theranostics, 2012
Photodynamic Therapy (PDT) provides an opportunity for treatment of various invasive tumors by the use of a cancer targeting photosensitizing agent and light of specific wavelengths. However, real-time monitoring of drug localization is desirable because
Shu-Chi Allison Yeh, Kevin R. Diamond, Michael S. Patterson, Zhaojun Nie, Joseph E. Hayward, Qiyin Fang
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Streamlined histone-based fluorescence lifetime imaging microscopy (FLIM) for studying chromatin organisation

Biology Open, 2018
Changes in chromatin structure are key determinants of genomic responses. Thus, methods that enable such measurements are instrumental for investigating genome regulation and function.
Alice Sherrard, Paul Bishop, Melanie Panagi, Maria Beatriz Villagomez, Dominic Alibhai, Abderrahmane Kaidi   +5 more
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Interrogation of Retinal Lipofuscin by Fluorescence Lifetime Imaging Microscopy

Journal of Biophotonics
ABSTRACT Age‐related macular degeneration is a disease that affects the middle part of the vision and involves pathological alterations in the retinal pigment epithelium. Accurate and timely evaluation of the retinal pigment epithelium is a cornerstone of effective treatment planning.
V. Andreev, M. Yakovleva, A. Kostyukov, V. Sokolova, V. Shcheslavskiy, G. Goltsman, T. Feldman, V. Kuzmin, M. Ostrovsky, P. Morozov   +9 more
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