Results 31 to 40 of about 30,206 (151)
Applications of combined spectral lifetime microscopy for biology
BioTechniques, 2006 Live cell imaging has been greatly advanced by the recent development of new fluorescence microscopy-based methods such as multiphoton laser-scanning microscopy, which can noninvasively image deep into live specimens and generate images of extrinsic and ...
Long Yan +3 more
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Building Accurate Intracellular Polarity Maps through Multiparametric Microscopy
Methods and Protocols, 2020 The precise knowledge of intracellular polarity, a physiological parameter that involves complex and intertwined intracellular mechanisms, may be relevant in the study of important diseases like cancer or Alzheimer’s.
M. Carmen Gonzalez-Garcia +3 more
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Rapid fluorescence lifetime imaging microscopy via few-photon imaging
APL PhotonicsConventional fluorescence lifetime imaging microscopy (FLIM) based on time-correlated single photon counting has great potential in various domains, notably in cellular biology, enabling comprehensive studies encompassing spatiotemporal dynamics and ...
Ming-Jie Sun +5 more
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Fluorescence lifetime imaging microscopy and its applications in skin cancer diagnosis [PDF]
Journal of Innovative Optical Health Sciences, 2019 Fluorescence lifetime (FLT) of fluorophores is sensitive to the changes in their surrounding microenvironment, and hence it can quantitatively reveal the physiological characterization of the tissue under investigation.
Lixin Liu +4 more
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Enhancing STED microscopy via fluorescence lifetime unmixing and filtering in two-species SPLIT-STED
Methods in MicroscopySimultaneous super-resolution imaging of multiple fluorophores remains a major challenge in STimulated Emission Depletion (STED) microscopy due to spectral overlap of STED-compatible fluorophores.
Deschênes Andréanne +8 more
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Lifetime map reconstruction in frequency-domain fluorescence lifetime imaging microscopy [PDF]
2012 19th IEEE International Conference on Image Processing, 2012 We propose a robust statistical framework for reconstructing lifetime map corrupted by vesicle motion in frequency domain FLIM imaging. Instrumental noise is taken into account to improve lifetime estimation. Robust M-estimators and MLestimators allow to jointly estimate motion and lifetime. Performances are demonstrated on simulated and real samples.
Roudot, Philippe +3 more
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Fluorescence lifetime imaging microscopy in the medical sciences
Protoplasma, 2014 The steady improvement in the imaging of cellular processes in living tissue over the last 10-15 years through the use of various fluorophores including organic dyes, fluorescent proteins and quantum dots, has made observing biological events common practice.
Ebrecht, Ren +2 more
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Frontiers in Physics, 2021 Though much of the interest in fluorescence in the past has been on measuring spectral qualities such as wavelength and intensity, there are two other highly useful intrinsic properties of fluorescence: lifetime (or decay) and anisotropy (or polarization)
Aric Bitton +3 more
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Rapid global fitting of large fluorescence lifetime imaging microscopy datasets.
PLoS ONE, 2013 Fluorescence lifetime imaging (FLIM) is widely applied to obtain quantitative information from fluorescence signals, particularly using Förster Resonant Energy Transfer (FRET) measurements to map, for example, protein-protein interactions.
Sean C Warren +9 more
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Image restoration for fluorescence lifetime imaging microscopy (FLIM)
Optics Express, 2008 Computational image restoration finds wide applicability for fluorescence intensity imaging. Relatively little work in this regard has been performed on FLIM images, which also suffer from diminished spatial resolution. In this work, we report two separate approaches to enhance FLIM image quality while maintaining lifetime accuracy.
Dhruv, Sud, Mary-Ann, Mycek
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