Scientific Reports, 2022 Label-free optical microscopy has matured as a noninvasive tool for biological imaging; yet, it is criticized for its lack of specificity, slow acquisition and processing times, and weak and noisy optical signals that lead to inaccuracies in ...
Rishyashring R. Iyer +10 more
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Manipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding
Nature Communications, 2020 Using fluorescence lifetime as the readout modality offers more reproducible and quantitative outputs compared to conventional fluorescent barcoding, being independent of sample concentration and measurement methods.
Yujie Xie +4 more
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Scientific Reports, 2021 We demonstrate that structured illumination microscopy has the potential to enhance fluorescence lifetime imaging microscopy (FLIM) as an early detection method for oral squamous cell carcinoma.
Taylor A. Hinsdale +8 more
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Multiphoton microscopy can visualize zonal damage and decreased cellular metabolic activity in hepatic ischemia-reperfusion injury in rats [PDF]
, 2011 Ischemia-reperfusion (I/R) injury is a common occurrence in liver surgery. In orthotopic transplantation, the donor liver is exposed to periods of ischemia and when oxygenated blood is reintroduced to the liver, oxidative stress may develop and lead to ...
Camilla A. Thorling +9 more
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Novel application of fluorescence lifetime and fluorescence microscopy enables quantitative access to subcellular dynamics in plant cells. [PDF]
PLoS ONE, 2009 BACKGROUND: Optical and spectroscopic technologies working at subcellular resolution with quantitative output are required for a deeper understanding of molecular processes and mechanisms in living cells.
Kirstin Elgass +5 more
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Fluorescence-Lifetime Imaging Techniques for Microscopy [PDF]
, 2003 CHAPTER 21 Fluorescence-Lifetime Imaging Techniques for Microscopy Chen Y. Dong, 1 Todd French, 2 Peter T. C. So, 1 C. Buehler, 1 Keith M. Berland, 3 and Enrico Gratton 1 Laboratory for Fluorescence Dynamics Department of Physics University of Illinois at Urbana-Champaign Urbana, Illinois 61801 Molecular Devices Corporation Sunnyvale, California 94089 ...
Dong, Chen Y +5 more
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Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy
Nature Communications, 2019 Nanosecond imaging techniques, such as fluorescence lifetime imaging microscopy (FLIM), are limited by low efficiency of current detectors. Here, the authors implement an electro-optic approach using Pockels cells for wide-field image gating and ...
Adam J. Bowman +3 more
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Simple phasor-based deep neural network for fluorescence lifetime imaging microscopy
Scientific Reports, 2021 Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to probe the molecular environment of fluorophores. The analysis of FLIM images is usually performed with time consuming fitting methods.
Laurent Héliot, Aymeric Leray
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Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy
The Scientific World Journal, 2013 Steady-state fluorence imaging and time-resolved fluorescence imaging are two important areas in fluorescence imaging research. Fluorescence lifetime imaging is an absolute measurement method which is independent of excitation laser intensity ...
Chao Liu +3 more
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Modern fluorescent techniques to investigate the mechanisms of lymphocyte activation [PDF]
The Ukrainian Biochemical Journal, 2015 Fluorescent proteins are promising tools for studying intracellular signaling processes in lymphocytes. This brief review summarizes fluorescence-based imaging techniques developed in recent years and discusses new methodological advances, such as ...
G. A. Liubchenko +2 more
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