Results 21 to 30 of about 30,206 (151)
Scientific Reports, 2022 Label-free optical microscopy has matured as a noninvasive tool for biological imaging; yet, it is criticized for its lack of specificity, slow acquisition and processing times, and weak and noisy optical signals that lead to inaccuracies in ...
Rishyashring R. Iyer +10 more
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Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy
Nature Communications, 2019 Nanosecond imaging techniques, such as fluorescence lifetime imaging microscopy (FLIM), are limited by low efficiency of current detectors. Here, the authors implement an electro-optic approach using Pockels cells for wide-field image gating and ...
Adam J. Bowman +3 more
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Scientific Reports, 2021 We demonstrate that structured illumination microscopy has the potential to enhance fluorescence lifetime imaging microscopy (FLIM) as an early detection method for oral squamous cell carcinoma.
Taylor A. Hinsdale +8 more
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Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy
The Scientific World Journal, 2013 Steady-state fluorence imaging and time-resolved fluorescence imaging are two important areas in fluorescence imaging research. Fluorescence lifetime imaging is an absolute measurement method which is independent of excitation laser intensity ...
Chao Liu +3 more
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Simple phasor-based deep neural network for fluorescence lifetime imaging microscopy
Scientific Reports, 2021 Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to probe the molecular environment of fluorophores. The analysis of FLIM images is usually performed with time consuming fitting methods.
Laurent Héliot, Aymeric Leray
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Modern fluorescent techniques to investigate the mechanisms of lymphocyte activation [PDF]
The Ukrainian Biochemical Journal, 2015 Fluorescent proteins are promising tools for studying intracellular signaling processes in lymphocytes. This brief review summarizes fluorescence-based imaging techniques developed in recent years and discusses new methodological advances, such as ...
G. A. Liubchenko +2 more
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Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells
Communications Biology, 2022 Oleksiievets et al. combine single-molecule localization super-resolution microscopy technique DNA-PAINT with fluorescence lifetime imaging to allow fast multi-target super-resolution imaging in one spectral region by separating two or three different ...
Nazar Oleksiievets +10 more
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Fluorescence Lifetime Imaging Microscopy Analysis of Isolated Melanosomes. [PDF]
ChemphyschemMelanosomes are organelles found in a wide variety of tissues throughout the animal kingdom. They contain a variety of biological molecules, but the dominant constituent is the pigment melanin, and many functions ascribed to melanosomes, such as photoprotection, are uniquely enabled by the chemical properties and structures of the melanins they contain.
Kizilov M +3 more
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Metabolic fingerprinting of bacteria by fluorescence lifetime imaging microscopy
Scientific Reports, 2017 Bacterial populations exhibit a range of metabolic states influenced by their environment, intra- and interspecies interactions. The identification of bacterial metabolic states and transitions between them in their native environment promises to ...
Arunima Bhattacharjee +3 more
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mCerulean3-Based Cameleon Sensor to Explore Mitochondrial Ca2+ Dynamics In Vivo
iScience, 2019 Summary: Genetically Encoded Ca2+ Indicators (GECIs) are extensively used to study organelle Ca2+ homeostasis, although some available probes are still plagued by a number of problems, e.g., low fluorescence intensity, partial mistargeting, and pH ...
Elisa Greotti +15 more
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