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Quantitative Imaging of Genetically Encoded Fluorescence Lifetime Biosensors
Biosensors, 2023 Genetically encoded fluorescence lifetime biosensors have emerged as powerful tools for quantitative imaging, enabling precise measurement of cellular metabolites, molecular interactions, and dynamic cellular processes.
Cong Quang Vu, Satoshi Arai
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Applications of fluorescence lifetime imaging in clinical medicine [PDF]
Journal of Innovative Optical Health Sciences, 2018 Fluorescence lifetime is not only associated with the molecular structure of fluorophores, but also strongly depends on the environment around them, which allows fluorescence lifetime imaging microscopy (FLIM) to be used as a tool for precise measurement
Zhanwen Wang +9 more
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Carbon Dots for Intracellular pH Sensing with Fluorescence Lifetime Imaging Microscopy
Nanomaterials, 2020 The monitoring of intracellular pH is of great importance for understanding intracellular trafficking and functions. It has various limitations for biosensing based on the fluorescence intensity or spectra study.
Maojia Huang +7 more
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Pinhole engineering based enhanced resolution (PEER) for fluorescence lifetime imaging microscopy [PDF]
Communications BiologyIn this article, we present a differential confocal microscopy technique based on pinhole engineering that significantly enhances both lateral resolution and axial sectioning depth.
Wonsang Hwang +4 more
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Multiplexed In Vivo Imaging with Fluorescence Lifetime‐Modulating Tags
Advanced ScienceFluorescence lifetime imaging microscopy (FLIM) opens new dimensions for highly multiplexed imaging in live cells and organisms using differences in fluorescence lifetime to distinguish spectrally identical fluorescent probes. Here, a set of fluorescence‐
Lina El Hajji +7 more
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Fluorescence lifetime imaging through scattering media
Scientific Reports, 2023 Fluorescence lifetime determination has proven to be useful, e.g. identification of molecules, quantitative estimation of species concentration and determination of temperatures.
Sebastian Nilsson +4 more
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Hyperdimensional Imaging Contrast Using an Optical Fiber
Sensors, 2021 Fluorescence properties of a molecule can be used to study the structural and functional nature of biological processes. Physical properties, including fluorescence lifetime, emission spectrum, emission polarization, and others, help researchers probe a ...
Jenu V. Chacko +4 more
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Aggregation-induced emission luminogen for in vivo three-photon fluorescence lifetime microscopic imaging [PDF]
Journal of Innovative Optical Health Sciences, 2019 Compared with visible light, near-infrared (NIR) light has deeper penetration in biological tissues. Three-photon fluorescence microscopy (3PFM) can effectively utilize the NIR excitation to obtain high-contrast images in the deep tissue.
Huwei Ni +5 more
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Frontiers in Oncology, 2021 Maximal safe resection is a key strategy for improving patient prognosis in the management of brain tumors. Intraoperative fluorescence guidance has emerged as a standard in the surgery of high-grade gliomas.
David Reichert +17 more
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Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy
The Scientific World Journal, 2013 Steady-state fluorence imaging and time-resolved fluorescence imaging are two important areas in fluorescence imaging research. Fluorescence lifetime imaging is an absolute measurement method which is independent of excitation laser intensity ...
Chao Liu +3 more
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