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Fluorescence lifetime imaging through scattering media
Scientific Reports, 2023 Fluorescence lifetime determination has proven to be useful, e.g. identification of molecules, quantitative estimation of species concentration and determination of temperatures.
Sebastian Nilsson +4 more
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Applications of fluorescence lifetime imaging in clinical medicine [PDF]
Journal of Innovative Optical Health Sciences, 2018 Fluorescence lifetime is not only associated with the molecular structure of fluorophores, but also strongly depends on the environment around them, which allows fluorescence lifetime imaging microscopy (FLIM) to be used as a tool for precise measurement
Zhanwen Wang +9 more
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Fluorescence lifetime imaging ophthalmoscopy
Progress in Retinal and Eye Research, 2017 Imaging techniques based on retinal autofluorescence have found broad applications in ophthalmology because they are extremely sensitive and noninvasive. Conventional fundus autofluorescence imaging measures fluorescence intensity of endogenous retinal fluorophores.
Chantal Dysli +2 more
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Carbon Dots for Intracellular pH Sensing with Fluorescence Lifetime Imaging Microscopy
Nanomaterials, 2020 The monitoring of intracellular pH is of great importance for understanding intracellular trafficking and functions. It has various limitations for biosensing based on the fluorescence intensity or spectra study.
Maojia Huang +7 more
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Multiplexed In Vivo Imaging with Fluorescence Lifetime‐Modulating Tags
Advanced ScienceFluorescence lifetime imaging microscopy (FLIM) opens new dimensions for highly multiplexed imaging in live cells and organisms using differences in fluorescence lifetime to distinguish spectrally identical fluorescent probes. Here, a set of fluorescence‐
Lina El Hajji +7 more
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An automated multiwell plate reading flim microscope for live cell autofluorescence lifetime assays [PDF]
Journal of Innovative Optical Health Sciences, 2014 Fluorescence lifetime imaging (FLIM) is increasingly used to read out cellular autofluorescence originating from the coenzyme NADH in the context of investigating cell metabolic state.
Douglas J. Kelly +8 more
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Interferometric excitation fluorescence lifetime imaging microscopy [PDF]
Nature CommunicationsFluorescence lifetime imaging microscopy (FLIM) is a well-established technique with numerous imaging applications. Yet, one of the limitations of FLIM is that it only provides information about the emitting state.
Pavel Malý +3 more
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High-throughput fluorescence lifetime imaging flow cytometry [PDF]
Nature CommunicationsFlow cytometry is a vital tool in biomedical research and laboratory medicine. However, its accuracy is often compromised by undesired fluctuations in fluorescence intensity.
Hiroshi Kanno +23 more
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Fluorescence lifetime imaging [PDF]
Analytical Biochemistry, 1992 We describe a new fluorescence imaging methodology in which the image contrast is derived from the fluorescence lifetime at each point in a two-dimensional image and not the local concentration and/or intensity of the fluorophore. In the present apparatus, lifetime images are created from a series of images obtained with a gain-modulated image ...
J R, Lakowicz +4 more
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Quantitative Imaging of Genetically Encoded Fluorescence Lifetime Biosensors
Biosensors, 2023 Genetically encoded fluorescence lifetime biosensors have emerged as powerful tools for quantitative imaging, enabling precise measurement of cellular metabolites, molecular interactions, and dynamic cellular processes.
Cong Quang Vu, Satoshi Arai
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