Results 31 to 40 of about 114,370 (293)

A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium

Nature Communications, 2021
Currently, genetically encoded calcium indicators are not suitable for direct quantification. Here the authors engineer a fluorescence lifetime imaging calcium biosensor, Turquoise Calcium Fluorescence LIfeTime Sensor (Tq-Ca-FLITS), and measure ...
Franka H. van der Linden, Eike K. Mahlandt, Janine J. G. Arts, Joep Beumer, Jens Puschhof, Saskia M. A. de Man, Anna O. Chertkova, Bas Ponsioen, Hans Clevers, Jaap D. van Buul, Marten Postma, Theodorus W. J. Gadella, Joachim Goedhart   +12 more
doaj   +1 more source

Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]

, 2012
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Abbe, Abbe, Ai, Airy, Alivisatos, Alivisatos, Ando, Aoki, Auksorius, Auzel, Auzel, Axelrod, Axelrod, Bacskai, Bader, Bader, Balse, Barroso, Bastiaens, Bates, Beaudouin, Beaurepaire, Becker, Benninger, Berezin, Berney, Betzig, Bianchini, Biju, Binnemans, Borst, Brakemann, Bretschneider, Bunt, Campagnola, Campagnola, Campbell, Cantor, Carrero, Chalfie, Chalfie, Chatterjee, Chen, Chen, Clapp, Clapp, Clapp, Cole, Coons, Dahan, Dedecker, Del Pozo, Deng, Denk, Diaspro, Diaspro, Dickson, Digman, Drobizhev, Drummen, Dundr, Dundr, Dunn, Dunn, Dunn, Durr, Dyba, Ehrlicher, Einstein, Elangovan, Elder, Ellenberg, Elsner, Feige, Fereidouni, Festenstein, Frommer, Förster, Förster, Gamelin, Garcia-Mira, Garcia-Parajo, Gerlich, Giese, Giordano, Goodson, Gopich, Gordon, Gregor P. C. Drummen, Grotjohann, Gu, Gunnlaugsson, Gurskaya, Gustafsson, Göppert-Mayer, Ha, Haugland, Heimstädt, Hellen C. Ishikawa-Ankerhold, Hemmila, Hiller, Hitakomate, Hofkens, Hofmann, Houtsmuller, Houtsmuller, Howell, Hu, Hu, Huang, Ishihama, Jacobson, Jares-Erijman, Jiang, Johannes, Johnson, Jonkman, Jose, Jovin, Jovin, Kaiser, Kambara, Kapustina, Karasawa, Kardash, Kasha, Kawashima, Kenworthy, Kerppola, Kerppola, Kerppola, Kerppola, Kimura, Kinoshita, Klonis, Kner, Knoblauch, Konig, Koppel, Koster, Kota, Koushik, Kremers, Kremers, Kuningas, Kuningas, Kuningas, Laforge, Lakowicz, Lakowicz, Larson, Lee, Lichtman, Liebman, Lippincott-Schwartz, Liu, Llères, Loura, Lukyanov, Lundin, Mansfield, Mathew-Fenn, Mattheyses, Matz, Medintz, Mills, Mitchell, Miyawaki, Miyawaki, Moerner, Moneron, Monetta, Mueller, Mueller, Nagai, Nehls, Ormö, Paddock, Parsons, Patterson, Patterson, Patterson, Pawley, Pawley, Persechini, Peters, Phair, Phair, Phillips, Piston, Piston, Planck, Pockwinse, Politz, Poo, Puliti, Rajaram, Rantanen, Reinhard, Reits, Reymann, Richard Ankerhold, Richards, Riven, Rizzo, Rizzo, Robinson, Roduner, Rotblat, Roy, Rust, Sakon, Sbalzarini, Schawlow, Schönle, Seabrooke, Seah, Selvin, Shaner, Shav-Tal, Shaw, Shchyolkina, Shimomura, Shimomura, Shroff, Shu, Shyu, Smith, So, Solon, Soukka, Soumpasis, Sprague, Sprague, Stasevich, Stelzer, Stepanenko, Stokes, Stryer, Stryer, Subach, Sun, Suyver, Swaminathan, Swift, Sönnichsen, Tagawa, Taylor, Thaler, Tian, Tian, Tinnefeld, Tisler, Tocanne, Tomosugi, Tripathi, Tsien, Tsien, Uphoff, Valentin, van Driel, Van Munster, Van Munster, van Munster, van Rheenen, van Royen, van Royen, van Royen, Varghese, Venetta, Vogelsang, von Wichert, Vosch, Wallrabe, Wang, Wang, Wang, Wang, Wang, Wang, Welch, White, Williams, Willig, Wouters, Wouters, Wouters, Wouters, Wright, Xia, Yang, Youvan, Zhang, Zhang, Zimmer, Zipfel   +295 more
core   +2 more sources

Quantifying nuclear wide chromatin compaction by phasor analysis of histone Förster resonance energy transfer (FRET) in frequency domain fluorescence lifetime imaging microscopy (FLIM) data. [PDF]

, 2020
The nanometer spacing between nucleosomes throughout global chromatin organisation modulates local DNA template access, and through continuous dynamic rearrangements, regulates genome function [1].
Gratton, Enrico, Hinde, Elizabeth, Liang, Zhen, Lou, Jieqiong, Scipioni, Lorenzo   +4 more
core   +3 more sources

Hadamard-transform fluorescence-lifetime imaging

Optics Express, 2016
We discuss a Hadamard-transform-based fluorescence-lifetime-imaging (HT-FLI) technique for fluorescence-lifetime-imaging microscopy (FLIM). The HT-FLI uses a Fourier-transform phase-modulation fluorometer (FT-PMF) for fluorescence-lifetime measurements, where the modulation frequency of the excitation light is swept linearly in frequency from zero to a
Mizuno, Takahiko, Iwata, Tetsuo
openaire   +2 more sources

Manipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding

Nature Communications, 2020
Using fluorescence lifetime as the readout modality offers more reproducible and quantitative outputs compared to conventional fluorescent barcoding, being independent of sample concentration and measurement methods.
Yujie Xie, Maria C. Arno, Jonathan T. Husband, Miquel Torrent-Sucarrat, Rachel K. O’Reilly   +4 more
doaj   +1 more source

Molecular rheometry: direct determination of viscosity in L-o and L-d lipid phases via fluorescence lifetime imaging [PDF]

, 2013
Understanding of cellular regulatory pathways that involve lipid membranes requires the detailed knowledge of their physical state and structure. However, mapping the viscosity and diffusion in the membranes of complex composition is currently a non ...
Agnieszka Olzyńska, Akashi, Alamiry, Benda, Beranova, Capoulade, Chen, Dertinger, Duncan R. Casey, Eggeling, Förster, Gokhan Yahioglu, Haidekker, Haidekker, Hoekstra, Hope, Ikonen, Jana Humpolíčková, Kahya, Kuimova, Kuimova, Kuimova, Levitt, Marina K. Kuimova, Martin Hof, Martin Štefl, Oscar Ces, Pan, Petrov, Philip Yip, Saffman, Sankaran, Schutz, Smith, Sykora, Veatch, Wohland, Yilei Wu   +37 more
core   +2 more sources

Fluorescence Lifetime Imaging of Apoptosis

Tomography, 2015
Genetically encoded fluorescence resonance energy transfer (FRET) reporters are powerful tools for analyzing cell signaling and function at single-cell resolution in standard 2D cell cultures, but these reporters rarely have been applied to 3D environments.
Xiao, Annie, Gibbons, Anne E., Luker, Kathryn E., Luker, Gary D.   +3 more
openaire   +2 more sources

Fluorescence Lifetime Endoscopy with a Nanosecond Time-Gated CAPS Camera with IRF-Free Deep Learning Method

Sensors
Fluorescence imaging has been widely used in fields like (pre)clinical imaging and other domains. With advancements in imaging technology and new fluorescent labels, fluorescence lifetime imaging is gradually gaining recognition.
Pooria Iranian, Thomas Lapauw, Thomas Van den Dries, Sevada Sahakian, Joris Wuts, Valéry Ann Jacobs, Jef Vandemeulebroucke, Maarten Kuijk, Hans Ingelberts   +8 more
doaj   +1 more source

Advanced Data Analysis for Fluorescence-Lifetime Single-Molecule Localization Microscopy

Frontiers in Bioinformatics, 2021
Fluorescence-lifetime single molecule localization microscopy (FL-SMLM) adds the lifetime dimension to the spatial super-resolution provided by SMLM. Independent of intensity and spectrum, this lifetime information can be used, for example, to quantify ...
Jan Christoph Thiele, Oleksii Nevskyi, Dominic A. Helmerich, Markus Sauer, Jörg Enderlein, Jörg Enderlein   +5 more
doaj   +1 more source

Light-field tomographic fluorescence lifetime imaging microscopy. [PDF]

Proc Natl Acad Sci U S A
Ma Y, Park J, Huang L, Sen C, Burri S, Bruschini C, Yang X, Cui Q, Cameron RB, Fishbein GA, Gomperts BN, Ozcan A, Charbon E, Gao L.   +13 more
europepmc   +2 more sources