Results 31 to 40 of about 41,989 (251)
Luminescent probes for luminescence lifetime sensing and imaging in live cells: a narrative review
Journal of Bio-X Research, 2020 . Luminescence (mostly fluorescence and phosphorescence) probes are a powerful tool widely used in the life sciences research. They can be used, for example, in the quantitative analysis of physiological parameters, visualization of different cell ...
Kaixuan Nie +4 more
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Label-Free Macroscopic Fluorescence Lifetime Imaging of Brain Tumors
Frontiers in Oncology, 2021 Advanced stage glioma is the most aggressive form of malignant brain tumors with a short survival time. Real-time pathology assisted, or image guided surgical procedures that eliminate tumors promise to improve the clinical outcome and prolong the lives ...
Maria Lukina +14 more
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Hadamard-transform fluorescence-lifetime imaging
Optics Express, 2016 We discuss a Hadamard-transform-based fluorescence-lifetime-imaging (HT-FLI) technique for fluorescence-lifetime-imaging microscopy (FLIM). The HT-FLI uses a Fourier-transform phase-modulation fluorometer (FT-PMF) for fluorescence-lifetime measurements, where the modulation frequency of the excitation light is swept linearly in frequency from zero to a
Mizuno, Takahiko, Iwata, Tetsuo
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An automated multiwell plate reading flim microscope for live cell autofluorescence lifetime assays [PDF]
Journal of Innovative Optical Health Sciences, 2014 Fluorescence lifetime imaging (FLIM) is increasingly used to read out cellular autofluorescence originating from the coenzyme NADH in the context of investigating cell metabolic state.
Douglas J. Kelly +8 more
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From mice to humans—divergent strategies for intestinal homeostasis and regeneration
FEBS Letters, EarlyView.Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa +2 more
wiley +1 more source
Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells
Communications Biology, 2022 Oleksiievets et al. combine single-molecule localization super-resolution microscopy technique DNA-PAINT with fluorescence lifetime imaging to allow fast multi-target super-resolution imaging in one spectral region by separating two or three different ...
Nazar Oleksiievets +10 more
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Molecular Probes for Fluorescence Lifetime Imaging [PDF]
Bioconjugate Chemistry, 2015 Visualization of biological processes and pathologic conditions at the cellular and tissue levels largely relies on the use of fluorescence intensity signals from fluorophores or their bioconjugates. To overcome the concentration dependency of intensity measurements, evaluate subtle molecular interactions, and determine biochemical status of ...
Pinaki, Sarder +2 more
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Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network
FEBS Letters, EarlyView.Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley +1 more source
Building Accurate Intracellular Polarity Maps through Multiparametric Microscopy
Methods and Protocols, 2020 The precise knowledge of intracellular polarity, a physiological parameter that involves complex and intertwined intracellular mechanisms, may be relevant in the study of important diseases like cancer or Alzheimer’s.
M. Carmen Gonzalez-Garcia +3 more
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PDT-Induced Variations of Radachlorin Fluorescence Lifetime in Living Cells In Vitro
Photonics, 2023 Variations in the fluorescence lifetimes of Radachlorin photosensitizers in HeLa and A549 cells, caused by photodynamic treatment, were studied using fluorescence lifetime imaging microscopy (FLIM).
Andrey V. Belashov +6 more
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