Results 21 to 30 of about 41,989 (251)

Stroboscopic fluorescence lifetime imaging

Optics Express, 2009
We report a fluorescence lifetime imaging technique that uses the time integrated response to a periodic optical excitation, eliminating the need for time resolution in detection. A Dirac pulse train of variable period is used to probe the frequency response of the total fluorescence per pulse leading to a frequency roll-off that is dependent on the ...
Holton, Mark D., Silvestre, Oscar, Errington, Rachel Jane, Smith, Paul James, Matthews, Daniel R., Rees, Paul, Summers, Huw D.   +6 more
openaire   +4 more sources

In vivo label-free mapping of the effect of a photosystem II inhibiting herbicide in plants using chlorophyll fluorescence lifetime

Plant Methods, 2017
Background In order to better understand and improve the mode of action of agrochemicals, it is useful to be able to visualize their uptake and distribution in vivo, non-invasively and, ideally, in the field.
Elizabeth Noble, Sunil Kumar, Frederik G. Görlitz, Chris Stain, Chris Dunsby, Paul M. W. French   +5 more
doaj   +1 more source

Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy

Scientific Reports, 2021
We demonstrate that structured illumination microscopy has the potential to enhance fluorescence lifetime imaging microscopy (FLIM) as an early detection method for oral squamous cell carcinoma.
Taylor A. Hinsdale, Bilal H. Malik, Shuna Cheng, Oscar R. Benavides, Maryellen L. Giger, John M. Wright, Paras B. Patel, Javier A. Jo, Kristen C. Maitland   +8 more
doaj   +1 more source

Macroscopic fluorescence-lifetime imaging of NADH and protoporphyrin IX improves the detection and grading of 5-aminolevulinic acid-stained brain tumors

Scientific Reports, 2020
Maximal safe tumor resection remains the key prognostic factor for improved prognosis in brain tumor patients. Despite 5-aminolevulinic acid-based fluorescence guidance the neurosurgeon is, however, not able to visualize most low-grade gliomas (LGG) and ...
Mikael T. Erkkilä, David Reichert, Johanna Gesperger, Barbara Kiesel, Thomas Roetzer, Petra A. Mercea, Wolfgang Drexler, Angelika Unterhuber, Rainer A. Leitgeb, Adelheid Woehrer, Angelika Rueck, Marco Andreana, Georg Widhalm   +12 more
doaj   +1 more source

A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium

Nature Communications, 2021
Currently, genetically encoded calcium indicators are not suitable for direct quantification. Here the authors engineer a fluorescence lifetime imaging calcium biosensor, Turquoise Calcium Fluorescence LIfeTime Sensor (Tq-Ca-FLITS), and measure ...
Franka H. van der Linden, Eike K. Mahlandt, Janine J. G. Arts, Joep Beumer, Jens Puschhof, Saskia M. A. de Man, Anna O. Chertkova, Bas Ponsioen, Hans Clevers, Jaap D. van Buul, Marten Postma, Theodorus W. J. Gadella, Joachim Goedhart   +12 more
doaj   +1 more source

Fluorescence Lifetime Endoscopy with a Nanosecond Time-Gated CAPS Camera with IRF-Free Deep Learning Method

Sensors
Fluorescence imaging has been widely used in fields like (pre)clinical imaging and other domains. With advancements in imaging technology and new fluorescent labels, fluorescence lifetime imaging is gradually gaining recognition.
Pooria Iranian, Thomas Lapauw, Thomas Van den Dries, Sevada Sahakian, Joris Wuts, Valéry Ann Jacobs, Jef Vandemeulebroucke, Maarten Kuijk, Hans Ingelberts   +8 more
doaj   +1 more source

Manipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding

Nature Communications, 2020
Using fluorescence lifetime as the readout modality offers more reproducible and quantitative outputs compared to conventional fluorescent barcoding, being independent of sample concentration and measurement methods.
Yujie Xie, Maria C. Arno, Jonathan T. Husband, Miquel Torrent-Sucarrat, Rachel K. O’Reilly   +4 more
doaj   +1 more source

Advanced Data Analysis for Fluorescence-Lifetime Single-Molecule Localization Microscopy

Frontiers in Bioinformatics, 2021
Fluorescence-lifetime single molecule localization microscopy (FL-SMLM) adds the lifetime dimension to the spatial super-resolution provided by SMLM. Independent of intensity and spectrum, this lifetime information can be used, for example, to quantify ...
Jan Christoph Thiele, Oleksii Nevskyi, Dominic A. Helmerich, Markus Sauer, Jörg Enderlein, Jörg Enderlein   +5 more
doaj   +1 more source

Dead-time correction of fluorescence lifetime measurements and fluorescence lifetime imaging

Optics Express, 2016
We present a comprehensive theory of dead-time effects on Time-Correlated Single Photon Counting (TCSPC) as used for fluorescence lifetime measurements, and develop a correction algorithm to remove these artifacts. We apply this algorithm to fluorescence lifetime measurements as well as to Fluorescence Lifetime Imaging Microscopy (FLIM), where rapid ...
Isbaner, Sebastian, Karedla, Narain, Ruhlandt, Daja, Stein, Simon Christoph, Chizhik, Anna, Gregor, Ingo, Enderlein, Jörg   +6 more
openaire   +3 more sources

The promise and peril of comparing fluorescence lifetime in biology revealed by simulations

eLife
Signaling dynamics are crucial in biological systems, and biosensor-based real-time imaging has revolutionized their analysis. Fluorescence lifetime imaging microscopy (FLIM) excels over the widely used fluorescence intensity imaging by allowing the ...
Pingchuan Ma, Peter Chen, Scott Sternson, Yao Chen   +3 more
doaj   +1 more source