Results 11 to 20 of about 41,989 (251)

Photoacoustic and fluorescence lifetime imaging of diatoms

open access: yesPhotoacoustics, 2020
Photoacoustic and fluorescent methods are used intensely in biology and medicine. These approaches can also be used to investigate unicellular diatom algae that are extremely important for Earth’s ecology.
Julijana Cvjetinovic   +8 more
doaj   +4 more sources

Fluorescence lifetime imaging [PDF]

open access: yesAnalytical Biochemistry, 1992
We describe a new fluorescence imaging methodology in which the image contrast is derived from the fluorescence lifetime at each point in a two-dimensional image and not the local concentration and/or intensity of the fluorophore. In the present apparatus, lifetime images are created from a series of images obtained with a gain-modulated image ...
J R, Lakowicz   +4 more
openaire   +2 more sources

IMAGING ARTIFACTS IN FLUORESCENCE LIFETIME IMAGING OPHTHALMOSCOPY

open access: yesRetina, 2021
Purpose: To investigate and quantify the influence of imaging artifacts on retinal fluorescence lifetime (FLIO) values and to provide helpful hints and tricks to avoid imaging artifacts and to improve FLIO image acquisition quality.
Chantal Dysli   +5 more
openaire   +3 more sources

Chemical fluorophores for fluorescence lifetime imaging.

open access: yesChem Soc Rev
Fluorescence lifetime imaging has emerged as a promising modality to extract molecular information from biological systems, providing detailed and semi-quantitative characterisation of subcellular microenvironments.
Lim C, Seah D, Vendrell M.
europepmc   +4 more sources

Fluorescence Lifetime Imaging of Apoptosis

open access: yesTomography, 2015
Genetically encoded fluorescence resonance energy transfer (FRET) reporters are powerful tools for analyzing cell signaling and function at single-cell resolution in standard 2D cell cultures, but these reporters rarely have been applied to 3D environments.
Xiao, Annie   +3 more
openaire   +2 more sources

Fluorescence lifetime imaging for explosive detection

open access: yesOptics Letters, 2023
In this Letter, a time-resolved 120 × 128 pixel single-photon avalanche diode (SPAD) sensor is used in conjunction with an array of organic semiconductor films as a means of detecting the presence of explosive vapors. Using the spatial and temporal resolution of the sensor, both fluorescence intensity and fluorescence lifetime can be monitored on a ...
Andrew B. Matheson   +4 more
openaire   +4 more sources

Fluorescence lifetime imaging for material studies [PDF]

open access: yesEPJ Web of Conferences
Fluorescence lifetime imaging microscopy (FLIM) is investigated as a contrast mechanism for the characterization of heterogeneous luminescent materials. A Ce:YAG glass-ceramic sample is used as a model system under epi-fluorescence excitation with a 450 ...
Zhang Zeyu   +2 more
doaj   +1 more source

Fluorescence Lifetime Spectroscopy and Imaging in Neurosurgery [PDF]

open access: yesIEEE Journal of Selected Topics in Quantum Electronics, 2012
Clinical outcome of patients diagnosed with primary brain tumor has been correlated with the extent of surgical resection. In treating this disease, the neurosurgeon must balance between an aggressive, radical resection and minimizing the loss of healthy, functionally significant brain tissue.
Laura, Marcu, Brad A, Hartl
openaire   +2 more sources

In Vivo Time Domain Optical Imaging of Renal Ischemia-Reperfusion Injury: Discrimination Based on Fluorescence Lifetime

open access: yesMolecular Imaging, 2007
Fluorescence lifetime is an intrinsic parameter of the fluorescent probe, independent of the probe concentration but sensitive to changes in the surrounding microenvironment.
Abedelnasser Abulrob   +4 more
doaj   +1 more source

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