, 2017 Two-photon excited fluorescence (TPEF) microscopy and fluorescence lifetime imaging (FLIM) are powerful imaging techniques in bio-molecular science. The need for elaborate light sources for TPEF and speed limitations for FLIM, however, hinder an even ...
Eibl, Matthias +4 more
core +1 more source
Luminescent probes for luminescence lifetime sensing and imaging in live cells: a narrative review
Journal of Bio-X Research, 2020 . Luminescence (mostly fluorescence and phosphorescence) probes are a powerful tool widely used in the life sciences research. They can be used, for example, in the quantitative analysis of physiological parameters, visualization of different cell ...
Kaixuan Nie +4 more
doaj +1 more source
GPU acceleration of time-domain fluorescence lifetime imaging [PDF]
, 2016 Fluorescence lifetime imaging microscopy (FLIM) plays a significant role in biological sciences, chemistry, and medical research. We propose a Graphic Processing Units (GPUs) based FLIM analysis tool suitable for high-speed and flexible time-domain FLIM ...
Chen, Yu +3 more
core +1 more source
The promise and peril of comparing fluorescence lifetime in biology revealed by simulations
eLifeSignaling dynamics are crucial in biological systems, and biosensor-based real-time imaging has revolutionized their analysis. Fluorescence lifetime imaging microscopy (FLIM) excels over the widely used fluorescence intensity imaging by allowing the ...
Pingchuan Ma +3 more
doaj +1 more source
Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells
Communications Biology, 2022 Oleksiievets et al. combine single-molecule localization super-resolution microscopy technique DNA-PAINT with fluorescence lifetime imaging to allow fast multi-target super-resolution imaging in one spectral region by separating two or three different ...
Nazar Oleksiievets +10 more
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Label-Free Macroscopic Fluorescence Lifetime Imaging of Brain Tumors
Frontiers in Oncology, 2021 Advanced stage glioma is the most aggressive form of malignant brain tumors with a short survival time. Real-time pathology assisted, or image guided surgical procedures that eliminate tumors promise to improve the clinical outcome and prolong the lives ...
Maria Lukina +14 more
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Recent Advances and the Potential for Clinical Use of Autofluorescence Detection of Extra-Ophthalmic Tissues [PDF]
, 2020 The autofluorescence (AF) characteristics of endogenous fluorophores allow the label-free assessment and visualization of cells and tissues of the human body.
Aigner, Felix +7 more
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Building Accurate Intracellular Polarity Maps through Multiparametric Microscopy
Methods and Protocols, 2020 The precise knowledge of intracellular polarity, a physiological parameter that involves complex and intertwined intracellular mechanisms, may be relevant in the study of important diseases like cancer or Alzheimer’s.
M. Carmen Gonzalez-Garcia +3 more
doaj +1 more source
PDT-Induced Variations of Radachlorin Fluorescence Lifetime in Living Cells In Vitro
Photonics, 2023 Variations in the fluorescence lifetimes of Radachlorin photosensitizers in HeLa and A549 cells, caused by photodynamic treatment, were studied using fluorescence lifetime imaging microscopy (FLIM).
Andrey V. Belashov +6 more
doaj +1 more source
Visualising apoptosis in live zebrafish using fluorescence lifetime imaging with optical projection tomography to map FRET biosensor activity in space and time [PDF]
, 2015 Fluorescence lifetime imaging (FLIM) combined with optical projection tomography (OPT) has the potential to map Förster resonant energy transfer (FRET) readouts in space and time in intact transparent or near transparent live organisms such as zebrafish ...
Alexandrov, Y +13 more
core +2 more sources