Results 61 to 70 of about 114,370 (293)

Probing subtle fluorescence dynamics in cellular proteins by streak camera based Fluorescence Lifetime Imaging Microscopy

, 2003
We report the cell biological applications of a recently developed multiphoton fluorescence lifetime imaging microscopy system using a streak camera (StreakFLIM).
Biener, Eva, Heckel, Robert, Herman, Brian, Krishnan, R. V., Zhang, Jian-Hua   +4 more
core   +1 more source

One size does not fit all: An in vitro evaluation of the effects of bezafibrate and medroxyprogesterone acetate on human SH‐SY5Y and U‐87 MG cancer cells

FEBS Open Bio, EarlyView.
Drugs previously repurposed to target blood cancers reduced neuroblastoma and glioblastoma cell growth and viability. However, their levels of anticancer activity were different and their clinical application may be problematic due to side effects at effective doses.
Abhishek Kharawatkar, Francesco Michelangeli, Farhat Latif Khanim, Christopher Martin Bunce, William Eustace Basil Johnson   +4 more
wiley   +1 more source

Rapid fluorescence lifetime imaging microscopy via few-photon imaging

APL Photonics
Conventional fluorescence lifetime imaging microscopy (FLIM) based on time-correlated single photon counting has great potential in various domains, notably in cellular biology, enabling comprehensive studies encompassing spatiotemporal dynamics and ...
Ming-Jie Sun, Yi-Cheng Zhang, Fang-Rui Lin, Shuai Wang, Li-Wei Liu, Jun-Le Qu   +5 more
doaj   +1 more source

Flavin fluorescence lifetime and autofluorescence optical redox ratio for improved visualization and classification of brain tumors

Frontiers in Oncology, 2023
PurposeModern techniques for improved tumor visualization have the aim to maximize the extent of resection during brain tumor surgery and thus improve patient prognosis.
David Reichert, David Reichert, Lisa I. Wadiura, Mikael T. Erkkilae, Johanna Gesperger, Johanna Gesperger, Alexandra Lang, Thomas Roetzer-Pejrimovsky, Jessica Makolli, Adelheid Woehrer, Marco Wilzbach, Christoph Hauger, Barbara Kiesel, Marco Andreana, Angelika Unterhuber, Wolfgang Drexler, Georg Widhalm, Rainer A. Leitgeb, Rainer A. Leitgeb   +18 more
doaj   +1 more source

Quantitative Imaging of Protein-Protein Interactions by Multiphoton Fluorescence Lifetime Imaging Microscopy using a Streak camera

, 2003
Fluorescence Lifetime Imaging Microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology.
A. Masuda, B. Herman, Becker, Clayton, Clegg, Cole, Elangovan, French, Gadella, Gerritsen, Harpur, Heya, Konig, Konig, Krishnan, Krishnan, Lakowicz, Mahajan, Neuberth, Oida, Periasamy, R. V. Krishnan, Schonle, Selvin, Squire, Squire, Straub, Sytsma, Szmacinski, Tahara, Tavare, V. E. Centonze, van Zandvoort, Verveer, Verveer, Verveer   +35 more
core   +1 more source

ALDOA Promotes Glycolysis and NLRP3/GSDMD Pyroptosis to Accelerate ALS Progression

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Amyotrophic lateral sclerosis (ALS) is characterized by progressive motor neuron degeneration. Glycolytic dysregulation is implicated in disease progression, yet the underlying mechanisms remain unclear. This study investigates how Aldolase A (ALDOA) drives ALS progression through glycolysis‐mediated motor neuron pyroptosis.
Kaixin Yan, Yan Jiang, Yuxuan Yong, Tianshuo Zhang, Niannian Zhang, Qianqian Zeng, Xue Gong, Li Meng, Fangfang Bi, Yongmin Liu   +9 more
wiley   +1 more source

Fluorescence lifetime imaging microscopy [PDF]

Nature Reviews Methods Primers
Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique offering profound insights into a broad spectrum of biological processes such as metabolic imaging, protein–protein interactions and live-cell intracellular dynamics. The future of FLIM appears promising, with continuous technological advancements for time-resolved measurements ...
Belen Torrado, Bruno Pannunzio, Leonel Malacrida, Michelle A. Digman   +3 more
openaire   +3 more sources

Assessment of Gate Width Size on Lifetime-Based Förster Resonance Energy Transfer Parameter Estimation

Photonics, 2015
Förster Resonance Energy Transfer (FRET) enables the observation of interactions at the nanoscale level through the use of fluorescence optical imaging techniques.
Sez-Jade Chen, Nattawut Sinsuebphon, Xavier Intes   +2 more
doaj   +1 more source

Covalent Organic Frameworks for Photocatalytic CO2 Reduction: Metal Integration Principles, Strategies and Functions

Advanced Functional Materials, EarlyView.
Covalent organic frameworks (COFs) with metals have been recognized as versatile platforms for photocatalytic CO2 reduction (CO2PRR). Herein, an overview of metal integration strategies for COFs is systematically summarized. Regulatory mechanisms and structure–activity relationships between metal integration and COF‐based CO2PRR are emphasized.
Jie He, Minxian Zhang, Jinqiang Zhang, Hongqi Sun, Shaobin Wang, Xiaoguang Duan   +5 more
wiley   +1 more source

Spectroscopically Well-Characterized RGD Optical Probe as a Prerequisite for Lifetime-Gated Tumor Imaging

Molecular Imaging, 2011
Labeling of RGD peptides with near-infrared fluorophores yields optical probes for noninvasive imaging of tumors overexpressing α v β 3 integrins. An important prerequisite for optimum detection sensitivity in vivo is strongly absorbing and highly ...
Julia Eva Mathejczyk, Jutta Pauli, Christian Dullin, Joanna Napp, Lutz-F. Tietze, Horst Kessler, Ute Resch-Genger, Frauke Alves   +7 more
doaj   +1 more source