Results 81 to 90 of about 114,370 (293)

Enhancing STED microscopy via fluorescence lifetime unmixing and filtering in two-species SPLIT-STED

Methods in Microscopy
Simultaneous super-resolution imaging of multiple fluorophores remains a major challenge in STimulated Emission Depletion (STED) microscopy due to spectral overlap of STED-compatible fluorophores.
Deschênes Andréanne, Ollier Antoine, Lafontaine Marie, Michaud-Gagnon Albert, Santiague Jeffrey-Gabriel Steavan, Bilodeau Anthony, Gagné Christian, De Koninck Paul, Lavoie-Cardinal Flavie   +8 more
doaj   +1 more source

Simultaneous multiplane imaging with reverberation multiphoton microscopy [PDF]

, 2019
Multiphoton microscopy (MPM) has gained enormous popularity over the years for its capacity to provide high resolution images from deep within scattering samples1.
Beaulieu, Devin R., Bifano, Thomas G., Davison, Ian G., Kılıç, Kıvılcım, Mertz, Jerome   +4 more
core   +1 more source

Disordered Carbon Shells Enable Shape Dependent Fluorescence Enhancement in Nanodiamond Quantum Sensors Revealed by Correlative TEM–Fluorescence Microscopy

Advanced Functional Materials, EarlyView.
Molten KNO2 treatment induces a lowrefractive index disordered carbon shell on fluorescent nanodiamonds, enhancing fluorescence emission while preserving spin coherence. Machine learningassisted correlative TEMPL enables direct single‐particle resolution of this enhancement relative to air‐oxidized nanodiamonds of similar morphology, establishing a ...
Parkarsh Kumar, Tom Vaillant, Mehran Kianinia, Haotian Wen, Adam Stewart, Peter J. Reece, Hugues A. Girard, Jean‐Charles Arnault, Huan‐Cheng Chang, Igor Aharonovich, Shery L. Y. Chang   +10 more
wiley   +1 more source

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

, 2013
We present an ex vivo study of temporally and spectrally resolved autofluorescence in a total of 47 endoscopic excision biopsy/resection specimens from colon, using pulsed excitation laser sources operating at wavelengths of 375 nm and 435 nm.
Chorvat, Chwirot, Chwirot, Cothren, Cothren, Cross, Curvers, De Beule, Drezek, Duraipandian, Eker, Fang, Georgakoudi, Georgakoudi, Glanzmann, Gono, Hilska, Hotouras, Huang, Jo, Kapadia, Kara, Kiesslich, Lambert, Li, Lin, Lovat, Maarek, Manning, Marcu, Mayinger, Mayinger, Mayinger, McGinty, Mizeret, Mycek, Nakaniwa, Nakashima, Palero, Palmer, Pfefer, Pitts, Ramanujam, Rex, Richards-Kortum, Schomacker, Schomacker, Schweitzer, Shao, Shim, Skala, Taplin, Thompson, Vishwasrao, Vogelstein, Wagnières, Wallace, Wang, Williams, Xiao, Yang, Zhuo, Zhuo, Zhuo, Zhuo, Zonios   +65 more
core   +1 more source

Some Secrets of Fluorescent Proteins: Distinct Bleaching in Various Mounting Fluids and Photoactivation of cyan fluorescent proteins at YFP-Excitation [PDF]

, 2011
Background The use of spectrally distinct variants of green fluorescent protein (GFP) such as cyan or yellow mutants (CFP and YFP, respectively) is very common in all different fields of life sciences, e.g. for marking specific proteins or cells
Johannes Schmid, Naila Malkani
core   +3 more sources

DNA‐Origami‐Assembled Rhodium Nanoantennas for Deep‐UV Label‐Free Single‐Protein Detection

Advanced Functional Materials, EarlyView.
Deep‐UV autofluorescence of single proteins in DNA origami‐based rhodium nanoantennas. Precisely positioned within the plasmonic nanogap, proteins retain their native state while their intrinsic emission is strongly amplified, enabling label‐free single‐molecule detection and opening new opportunities for highly sensitive and specific biosensing ...
Nicco Corduri, Malavika Kayyil Veedu, Yifan Yu, Yanqiu Zou, Jie Liu, Denis Garoli, Guillermo P. Acuna, Jérôme Wenger, Karol Kołątaj   +8 more
wiley   +1 more source

Low-cost luminescence lifetime system for medical diagnostics

JPhys Photonics
Luminescence lifetimes provide insights into the local microenvironment of luminescent molecules, enabling precise measurements that can be leveraged for biomedical imaging and sensing applications.
Lakhvir Singh, Melody Yeh, Linghao Hu, Brian Ko, Michael J McShane, Alex J Walsh   +5 more
doaj   +1 more source

Cellular trafficking of Sn-2 phosphatidylcholine prodrugs studied with fluorescence lifetime imaging and super-resolution microscopy [PDF]

, 2018
While ...
Achilefu, Samuel, Cui, Grace, Lanza, Gregory M, Lew, Matthew D, Lu, Jin, Maji, Dolonchampa, Pan, Dipanjan, Sarder, Pinaki, Schmieder, Anne H, Yang, Xiaoxia   +9 more
core   +1 more source

Ruthenium polypyridyl peptide conjugates: membrane permeable probes for cellular imaging [PDF]

, 2008
Two novel polyarginine labelled ruthenium polypyridyl dyes are reported, one conjugated to five, (Ru-Ahx-R5), and one to eight arginine residues, (Ru-Ahx-R8). Both complexes exhibit long-lived, intense, and oxygen sensitive luminescence.
Amoroso, Aylward, Bai, Brunner, Dobrucki, Fischer, Fuchs, Fuchs, Fulda, Futaki, Kale, Kielar, Kielar, Lakowicz, Lissi, Lo, Marc Devocelle, Mendoza, Mendoza, Murray, Musatkinaa, Niamh Moran, Pellegrin, Robert J. Forster, Santra, Tia E. Keyes, Ute Neugebauer, William Signac, Xu, Yann Pellegrin, Zhang, Zhong, Zhong   +32 more
core   +1 more source

Molecular Probes for Fluorescence Lifetime Imaging [PDF]

Bioconjugate Chemistry, 2015
Visualization of biological processes and pathologic conditions at the cellular and tissue levels largely relies on the use of fluorescence intensity signals from fluorophores or their bioconjugates. To overcome the concentration dependency of intensity measurements, evaluate subtle molecular interactions, and determine biochemical status of ...
Pinaki, Sarder, Dolonchampa, Maji, Samuel, Achilefu   +2 more
openaire   +2 more sources