Results 71 to 80 of about 114,370 (293)
Fluorescence decay data analysis correcting for detector pulse pile-up at very high count rates
, 2017 Using Time-Correlated Single Photon Counting (TCSPC) for the purpose of fluorescence lifetime measurements is usually limited in speed due to pile-up.
Dowler, Rhys +5 more
core +1 more source
Label-Free Metabolic Classification of Single Cells in Droplets Using the Phasor Approach to Fluorescence Lifetime Imaging Microscopy. [PDF]
, 2019 Characterization of single cell metabolism is imperative for understanding subcellular functional and biochemical changes associated with healthy tissue development and the progression of numerous diseases.
Aghaamoo, Mohammad +4 more
core +1 more source
Nanothermometry in Living Cells: Physical Limits, Conceptual and Material Challenges
Advanced Functional Materials, EarlyView.Heat and temperature are fundamental to life. When nanothermometers began probing regions as small as a living cell, they triggered controversial claims of large intracellular temperature gradients. We review physical constraints energy‐conservation, entropy production, thermodynamic fluctuations, and molecular dynamics.
Taras Plakhotnik
wiley +1 more source
Color Routing and Beam Steering of Single‐Molecule Emission with a Spherical Silicon Nanoantenna
Advanced Functional Materials, EarlyView.We experimentally demonstrate broadband directional emission from single molecules using a single spherical silicon nanoparticle assembled via DNA origami. By varying nanoparticle (NP) size and emitter position, we achieve unidirectional emission, beam steering, and color routing at the nanoscale, revealing modal interference as the underlying ...
María Sanz‐Paz +9 more
wiley +1 more source
Scanning emitter lifetime imaging microscopy for spontaneous emission control [PDF]
, 2011 We report an experimental technique to map and exploit the local density of optical states of arbitrary planar nano-photonic structures. The method relies on positioning a spontaneous emitter attached to a scanning probe deterministically and reversibly ...
A. Femius Koenderink +3 more
core +2 more sources
Mechanochromism of Glassy Polymers Enabled by a Loop‐Forming Supramolecular Mechanophore
Advanced Functional Materials, EarlyView.The supramolecular mechanophore LOOP is covalently introduced into glassy polymers, capable of reporting force‐induced pre‐failure damage and local fracture events in polymer glasses. Under different mechanical deformation modes, including grinding, tensile stretching, and scratching, LOOP probes irreversible events in polymer glasses through a ...
Linlin Deng +6 more
wiley +1 more source
Advanced Functional Materials, EarlyView.This article presents a photo‐responsive nanoparticle platform that precisely triggers copper‐dependent cuproptotic cell death in (drug‐resistant) cancer cells. The system remains stable and inactive in the dark but releases cytotoxic species upon red‐light irradiation, achieving potent activity in drug‐resistant breast cancer cells.
Ricarda Zimmermann +2 more
wiley +1 more source
Imaging mitochondrial membrane potential via concentration-dependent fluorescence lifetime changes
Nature CommunicationsMitochondria are central to cellular metabolism. Various fluorescence tools have been developed for imaging the mitochondrial environment. Yet, new reporters and imaging methods for directly reading the mitochondrial status are needed for high spatial ...
Dilizhatai Saimi +12 more
doaj +1 more source
Deep compressive macroscopic fluorescence lifetime imaging [PDF]
2018 IEEE 15th International Symposium on Biomedical Imaging (ISBI 2018), 2018 Compressive Macroscopic Fluorescence Lifetime Imaging (MFLI) is a novel technical implementation that enables monitoring multiple molecular interactions in macroscopic scale. Especially, we reported recently on the development of a hyperspectral wide-field time-resolved single-pixel imaging platform that facilitates whole-body in vivo lifetime imaging ...
Yao, Ruoyang +3 more
openaire +2 more sources
Photon Avalanching Nanoparticles: The Next Generation of Upconverting Nanomaterials?
Advanced Functional Materials, EarlyView.This Perspective outlines the mechanistic foundations that enable photon‐avalanche (PA) behavior in lanthanide nanomaterials and contrasts them with emerging application spaces and forward‐looking design strategies. By bridging threshold engineering, energy‐transfer dynamics, and materials engineering, we provide a coherent roadmap for advancing the ...
Kimoon Lee +7 more
wiley +1 more source