Visualising varnish removal for conservation of paintings by fluorescence lifetime imaging (FLIM) [PDF]
Heritage Science, 2023 The removal of varnish from the surface is a key step in painting conservation. Varnish removal is traditionally monitored by examining the painting surface under ultraviolet illumination.
Christine B. Wilda +5 more
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A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos [PDF]
Scientific ReportsA wide-field microscope with epi-fluorescence and selective plane illumination was combined with a single-photon avalanche diode (SPAD) array camera to enable live-cell fluorescence lifetime imaging (FLIM) using time-correlated single-photon counting ...
Jakub Nedbal +7 more
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Quantitative Imaging of Genetically Encoded Fluorescence Lifetime Biosensors
Biosensors, 2023 Genetically encoded fluorescence lifetime biosensors have emerged as powerful tools for quantitative imaging, enabling precise measurement of cellular metabolites, molecular interactions, and dynamic cellular processes.
Cong Quang Vu, Satoshi Arai
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Scientific Reports, 2021 We demonstrate that structured illumination microscopy has the potential to enhance fluorescence lifetime imaging microscopy (FLIM) as an early detection method for oral squamous cell carcinoma.
Taylor A. Hinsdale +8 more
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Carbon Dots for Intracellular pH Sensing with Fluorescence Lifetime Imaging Microscopy
Nanomaterials, 2020 The monitoring of intracellular pH is of great importance for understanding intracellular trafficking and functions. It has various limitations for biosensing based on the fluorescence intensity or spectra study.
Maojia Huang +7 more
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Wide-Field Multi-Parameter FLIM: long-term minimal invasive observation of proteins in living cells. [PDF]
PLoS ONE, 2011 Time-domain Fluorescence Lifetime Imaging Microscopy (FLIM) is a remarkable tool to monitor the dynamics of fluorophore-tagged protein domains inside living cells.
Marco Vitali +9 more
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An automated multiwell plate reading flim microscope for live cell autofluorescence lifetime assays [PDF]
Journal of Innovative Optical Health Sciences, 2014 Fluorescence lifetime imaging (FLIM) is increasingly used to read out cellular autofluorescence originating from the coenzyme NADH in the context of investigating cell metabolic state.
Douglas J. Kelly +8 more
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Simple and Robust Deep Learning Approach for Fast Fluorescence Lifetime Imaging
Sensors, 2022 Fluorescence lifetime imaging (FLIM) is a powerful tool that provides unique quantitative information for biomedical research. In this study, we propose a multi-layer-perceptron-based mixer (MLP-Mixer) deep learning (DL) algorithm named FLIM-MLP-Mixer ...
Quan Wang +6 more
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Hyperdimensional Imaging Contrast Using an Optical Fiber
Sensors, 2021 Fluorescence properties of a molecule can be used to study the structural and functional nature of biological processes. Physical properties, including fluorescence lifetime, emission spectrum, emission polarization, and others, help researchers probe a ...
Jenu V. Chacko +4 more
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Fluorescence lifetime imaging of fluorescent proteins as an effective quantitative tool for noninvasive study of intracellular processes [PDF]
Journal of Innovative Optical Health Sciences, 2018 Fluorescence lifetime imaging (FLIM) is an effective noninvasive bioanalytical tool based on measuring fluorescent lifetime of fluorophores. A growing number of FLIM studies utilizes genetically engineered fluorescent proteins targeted to specific ...
Svitlana M. Levchenko +2 more
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