Results 31 to 40 of about 6,539 (187)

Interferometric excitation fluorescence lifetime imaging microscopy

Nature Communications
Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique with numerous imaging applications. Yet, one of the limitations of FLIM is that it only provides information about the emitting state.
Pavel Malý, Dita Strachotová, Aleš Holoubek, Petr Heřman   +3 more
doaj   +1 more source

Label-free characterization of single extracellular vesicles using two-photon fluorescence lifetime imaging microscopy of NAD(P)H

Scientific Reports, 2021
The heterogeneous nature of extracellular vesicles (EVs) creates the need for single EV characterization techniques. However, many common biochemical and functional EV analysis techniques lack single EV resolution.
Janet E. Sorrells, Elisabeth M. Martin, Edita Aksamitiene, Prabuddha Mukherjee, Aneesh Alex, Eric J. Chaney, Marina Marjanovic, Stephen A. Boppart   +7 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Development of fluorescence lifetime biosensors for ATP, cAMP, citrate, and glucose using the mTurquoise2-based platform

Cell Reports: Methods
Summary: Single-fluorescent protein (FP)-based FLIM (fluorescence lifetime imaging microscopy) biosensors can visualize intracellular processes quantitatively. They require a single wavelength for detection, which facilitates multi-color imaging. However,
Chongxia Zhong, Satoshi Arai, Yasushi Okada   +2 more
doaj   +1 more source

FLIM as a Promising Tool for Cancer Diagnosis and Treatment Monitoring

Nano-Micro Letters, 2021
Fluorescence lifetime imaging microscopy (FLIM) has been rapidly developed over the past 30 years and widely applied in biomedical engineering. Recent progress in fluorophore-dyed probe design has widened the application prospects of fluorescence ...
Yuzhen Ouyang, Yanping Liu, Zhiming M. Wang, Zongwen Liu, Minghua Wu   +4 more
doaj   +1 more source

Insights into Metabolic Activity and Structure of the Retina through Multiphoton Fluorescence Lifetime Imaging Microscopy in Mice

Cells, 2022
Fluorescence lifetime imaging microscopy (FLIM) evaluates the metabolic state of tissue based on reduced nicotinamide adenine dinucleotide (NAD(P)H) and flavin adenine dinucleotide (FAD).
Niranjana Kesavamoorthy, Jason A. Junge, Scott E. Fraser, Hossein Ameri   +3 more
doaj   +1 more source

Endoscopic tissue characterization by frequency-domain fluorescence lifetime imaging (FD-FLIM) [PDF]

Lasers in Medical Science, 1997
Tissue characterization by endoscopic fluorescence imaging of endogenous or exogenous fluorochromes is a promising method for early cancer detection. However, the steady-state fluorescence contrast between healthy tissue and lesions such as early-stage carcinomas is generally poor.
Mizeret, J., Wagnières, G., Stepinac, T., Van Den Bergh, H.   +3 more
openaire   +4 more sources

Photoluminescence Reshaping via Structural Confinement and Local Density of Optical States Control in Ir(III)‐Doped Biodegradable Metasurfaces

Advanced Materials Technologies, EarlyView.
Phosphorescent biodegradable metasurface showing vibronic selection and spectral photoluminescence narrowing. Anti‐correlation between experimental LDOS and measured lifetimes. ABSTRACT Metasurfaces incorporating phosphorescent emitters offer a route to engineer radiative and vibronic processes beyond chemical design.
Vincenzo Caligiuri, Andrea Smeriglio, Svetlana Siprova, Olga Favale, Francesca Leone, Roberto Termine, Debora Policastro, Alessandra Crispini, Attilio Golemme, Nicolas Godbert, Iolinda Aiello, Antonio De Luca   +11 more
wiley   +1 more source

Overcoming photon and spatiotemporal sparsity in fluorescence lifetime imaging with SparseFLIM

Communications Biology
Fluorescence lifetime imaging microscopy (FLIM) provides quantitative readouts of biochemical microenvironments, holding great promise for biomedical imaging.
Binglin Shen, Yuan Lu, Fangyin Guo, Fangrui Lin, Rui Hu, Feng Rao, Junle Qu, Liwei Liu   +7 more
doaj   +1 more source

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells [PDF]

FEBS Open Bio
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Hink M.
europepmc   +2 more sources