Results 11 to 20 of about 6,539 (187)

Multiplexable fluorescence lifetime imaging (FLIM) probes for Abl and Src-family kinases. [PDF]

Chem Commun (Camb), 2020
Here we describe development of cell-penetrating peptide substrates for fluorescence lifetime imaging (FLIM) of Abl and Src-family kinase activities, applied to mapping single cell and subcellular dynamics of multiple kinases in live cells.
Jena S, Damayanti NP, Tan J, Pratt ED, Irudayaraj JMK, Parker LL.   +5 more
europepmc   +4 more sources

Reversible, Chemically Gated FRET via Ligand-Activated Acceptors. [PDF]

Adv Sci (Weinh)
Fluorogen binding turns the compact FAST tag into a tunable FRET acceptor, with ligand chemistry programming spectral overlap and excited‐state lifetimes. Fluorescence‐lifetime imaging reads out energy transfer through the shortened donor lifetime, while simply adding or washing out the dye switches FRET on and off, reversibly mapping the supramodular ...
Singh N, Nayak SR, Mohanty SK, Hanumantharaju A, Shaju A, Jose M, Penmatsa A, Nair D.   +7 more
europepmc   +2 more sources

Carbohydrate-Mediated Cellular Uptake of Boronic Acid Hybrids In Cellulo and Insulin-Deficient Zebrafish: Live Imaging and Application of Multiphoton FLIM. [PDF]

Adv Sci (Weinh)
Boronic acid‐based fluorescent hybrids have been used as part of a versatile platform for real‐time metabolic bioimaging across cellular and organismal scales. Including glucose‐responsive imaging in living cells and in an insulin‐deficient vertebrate model, this work opens new opportunities for the development of precision diagnostics, metabolic ...
Ge H, Fossey JS, Calatayud DG, Flower SE, Wang S, Aitessaid H, Tvardauskaite M, Dudzic J, Jewell M, Pourzand C, Botchway SW, Gurevich D, James TD, Pascu SI.   +13 more
europepmc   +2 more sources

Convolutional neural network denoising in fluorescence lifetime imaging microscopy (FLIM) [PDF]

Multiphoton Microscopy in the Biomedical Sciences XXI, 2021
Fluorescence lifetime imaging microscopy (FLIM) systems are limited by their slow processing speed, low signal-to-noise ratio (SNR), and expensive and challenging hardware setups. In this work, we demonstrate applying a denoising convolutional network to improve FLIM SNR.
Varun Mannam, Yide Zhang, Xiaotong Yuan, Takashi Hato, Pierre C. Dagher, Evan L. Nichols, Cody J. Smith, Kenneth W. Dunn, Scott S. Howard   +8 more
openaire   +2 more sources

Fluorescence Lifetime Imaging Microscopy (FLIM) Data Analysis withTIMP [PDF]

Journal of Statistical Software, 2007
Fluorescence Lifetime Imaging Microscopy (FLIM) allows fluorescence lifetime images of biological objects to be collected at 250 nm spatial resolution and at (sub-)nanosecond temporal resolution. Often ncomp kinetic processes underlie the observed fluorescence at all locations, but the intensity of the fluorescence associated with each process varies ...
Laptenok, S., Mullen, K.M., Borst, J.W., van Stokkum, I.H.M., Apanasovich, V.V., Visser, A.J.W.G.   +5 more
openaire   +5 more sources

A pH-Responsive fluorescence lifetime probe reveals GOT1 phase separation in cellular anti-senescence. [PDF]

Smart Mol
Acidic pH triggers GOT1 phase separation via IDR1, recruiting ME1 to form ROS‐scavenging condensates that combat senescence, revealed by a FLIM‐based pH probe. Abstract Cellular senescence involves progressive acidification, but how cells sense and adapt to this pH shift remains unclear.
Chen Z, Li J, Bao Y, Wang M, Chen H, Zhang M, Ding L, Wu S, Shen B.   +8 more
europepmc   +2 more sources

A Rapid Method for Detecting Microplastics Based on Fluorescence Lifetime Imaging Technology (FLIM) [PDF]

Toxics, 2022
With the increasing use and release of plastic products, microplastics have rapidly accumulated in ecological environments. When microplastics enter the food chain, they cause serious harm to organisms and humans. Microplastics pollution has become a growing concern worldwide; however, there is still no standardized method for rapidly and accurately ...
Zhou, Fang, Wang, Xin, Wang, Guangxin, Zuo, Yanxia   +3 more
openaire   +3 more sources

Two-Photon Fluorescence and Second-Harmonic Generation Microscopy: Principles and Biomedical Applications. [PDF]

J Biophotonics
Compared with single‐photon methods, TPM enables deep‐tissue imaging using near‐infrared light, with excitation confined to the focal plane, thereby reducing photodamage. SHG microscopy images noncentrosymmetric biological structures such as collagen and myosin without fluorescent labels, offering direct structural contrast.
Makkithaya KN, Raju G, Das S, Patil A, Kumar R, Kistenev YV, Zhuo GY, Mazumder N.   +7 more
europepmc   +2 more sources

Aggregation-induced emission luminogen for in vivo three-photon fluorescence lifetime microscopic imaging [PDF]

Journal of Innovative Optical Health Sciences, 2019
Compared with visible light, near-infrared (NIR) light has deeper penetration in biological tissues. Three-photon fluorescence microscopy (3PFM) can effectively utilize the NIR excitation to obtain high-contrast images in the deep tissue.
Huwei Ni, Zicong Xu, Dongyu Li, Ming Chen, Ben Zhong Tang, Jun Qian   +5 more
doaj   +1 more source

Improving fluorescence lifetime imaging microscopy phasor accuracy using convolutional neural networks

Frontiers in Bioinformatics, 2023
Introduction: Although a powerful biological imaging technique, fluorescence lifetime imaging microscopy (FLIM) faces challenges such as a slow acquisition rate, a low signal-to-noise ratio (SNR), and high cost and complexity.
Varun Mannam, Jacob P. Brandt, Cody J. Smith, Xiaotong Yuan, Scott Howard   +4 more
doaj   +1 more source