Results 21 to 30 of about 6,539 (187)

Image restoration for fluorescence lifetime imaging microscopy (FLIM)

Optics Express, 2008
Computational image restoration finds wide applicability for fluorescence intensity imaging. Relatively little work in this regard has been performed on FLIM images, which also suffer from diminished spatial resolution. In this work, we report two separate approaches to enhance FLIM image quality while maintaining lifetime accuracy.
Dhruv, Sud, Mary-Ann, Mycek
openaire   +2 more sources

Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy

The Scientific World Journal, 2013
Steady-state fluorence imaging and time-resolved fluorescence imaging are two important areas in fluorescence imaging research. Fluorescence lifetime imaging is an absolute measurement method which is independent of excitation laser intensity ...
Chao Liu, Xinwei Wang, Yan Zhou, Yuliang Liu   +3 more
doaj   +1 more source

Applications of fluorescence lifetime imaging in clinical medicine [PDF]

Journal of Innovative Optical Health Sciences, 2018
Fluorescence lifetime is not only associated with the molecular structure of fluorophores, but also strongly depends on the environment around them, which allows fluorescence lifetime imaging microscopy (FLIM) to be used as a tool for precise measurement
Zhanwen Wang, Yanping Zheng, Deqiang Zhao, Ziwei Zhao, Lixin Liu, Artem Pliss, Feiqi Zhu, Jun Liu, Junle Qu, Ping Luan   +9 more
doaj   +1 more source

Contribution of autofluorescence from intracellular proteins in multiphoton fluorescence lifetime imaging

Scientific Reports, 2022
Multiphoton fluorescence lifetime imaging microscopy (MPM-FLIM) is extensively proposed as a non-invasive optical method to study tissue metabolism.
Monika Malak, Jeemol James, Julie Grantham, Marica B. Ericson   +3 more
doaj   +1 more source

Fluorescence lifetime imaging for material studies [PDF]

EPJ Web of Conferences
Fluorescence lifetime imaging microscopy (FLIM) is investigated as a contrast mechanism for the characterization of heterogeneous luminescent materials. A Ce:YAG glass-ceramic sample is used as a model system under epi-fluorescence excitation with a 450 ...
Zhang Zeyu, Hofmann Meike, Sinzinger Stefan   +2 more
doaj   +1 more source

Refractive index sensing using Fluorescence Lifetime Imaging (FLIM)

Journal of Physics: Conference Series, 2006
The average fluorescence lifetime of GFP in solution is a function of the refractive index of its environment. Here, we demonstrate that this also appears to be the case for GFP-tagged proteins in cells. Using TCSPC-based FLIM with a scanning confocal microscope, we image GFP-tagged proteins in fixed cells in different media.
Carolyn Tregidgo, Klaus Suhling
openaire   +1 more source

Fluorescence lifetime imaging microscopy and its applications in skin cancer diagnosis [PDF]

Journal of Innovative Optical Health Sciences, 2019
Fluorescence lifetime (FLT) of fluorophores is sensitive to the changes in their surrounding microenvironment, and hence it can quantitatively reveal the physiological characterization of the tissue under investigation.
Lixin Liu, Qianqian Yang, Meiling Zhang, Zhaoqing Wu, Ping Xue   +4 more
doaj   +1 more source

FLIMJ: An open-source ImageJ toolkit for fluorescence lifetime image data analysis.

PLoS ONE, 2020
In the field of fluorescence microscopy, there is continued demand for dynamic technologies that can exploit the complete information from every pixel of an image.
Dasong Gao, Paul R Barber, Jenu V Chacko, Md Abdul Kader Sagar, Curtis T Rueden, Aivar R Grislis, Mark C Hiner, Kevin W Eliceiri   +7 more
doaj   +1 more source

Combining Multicolor FISH with Fluorescence Lifetime Imaging for Chromosomal Identification and Chromosomal Sub Structure Investigation

Frontiers in Molecular Biosciences, 2021
Understanding the structure of chromatin in chromosomes during normal and diseased state of cells is still one of the key challenges in structural biology.
Archana Bhartiya, Archana Bhartiya, Ian Robinson, Ian Robinson, Mohammed Yusuf, Mohammed Yusuf, Mohammed Yusuf, Stanley W. Botchway   +7 more
doaj   +1 more source

Mutant p53R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation

Molecular Oncology, EarlyView.
How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Mei Chee Lim, Alvin Kunyao Guo, Yoko Itahana, Srinivasaraghavan Kannan, Valerie Goh, Mingmin Ong, Terina Yan Wen Tay, Weiying Ye, Sabhashina Radha Krishnan, Jun Nishiyama, Chandra S Verma, Koji Itahana   +11 more
wiley   +1 more source