Results 51 to 60 of about 6,539 (187)

High-throughput fluorescence lifetime imaging flow cytometry

Nature Communications
Flow cytometry is a vital tool in biomedical research and laboratory medicine. However, its accuracy is often compromised by undesired fluctuations in fluorescence intensity.
Hiroshi Kanno, Kotaro Hiramatsu, Hideharu Mikami, Atsushi Nakayashiki, Shota Yamashita, Arata Nagai, Kohki Okabe, Fan Li, Fei Yin, Keita Tominaga, Omer Faruk Bicer, Ryohei Noma, Bahareh Kiani, Olga Efa, Martin Büscher, Tetsuichi Wazawa, Masahiro Sonoshita, Hirofumi Shintaku, Takeharu Nagai, Sigurd Braun, Jessica P. Houston, Sherif Rashad, Kuniyasu Niizuma, Keisuke Goda   +23 more
doaj   +1 more source

The Nature of Cell‐Penetrating Poly(disulfide)s

Angewandte Chemie, EarlyView.
The mode of action of cell‐penetrating poly(disulfide)s (CPDs) is elucidated to understand thiol‐mediated uptake (TMU) in general and guide future efforts to control cellular entry for chemicals delivery and pathogen defense. ABSTRACT Cell‐penetrating poly(disulfide)s (CPDs) are the most popular thiol‐mediated uptake (TMU) tools for delivering attached
Saidbakhrom Saidjalolov, Giacomo Renno, Yuheng Zhang, Michael Cognet, Justin Neuman, Andreas Hennig, Naomi Sakai, Stefan Matile   +7 more
wiley   +2 more sources

Rapid fluorescence lifetime imaging microscopy via few-photon imaging

APL Photonics
Conventional fluorescence lifetime imaging microscopy (FLIM) based on time-correlated single photon counting has great potential in various domains, notably in cellular biology, enabling comprehensive studies encompassing spatiotemporal dynamics and ...
Ming-Jie Sun, Yi-Cheng Zhang, Fang-Rui Lin, Shuai Wang, Li-Wei Liu, Jun-Le Qu   +5 more
doaj   +1 more source

In vivo melanin 3D quantification and z-epidermal distribution by multiphoton FLIM, phasor and Pseudo-FLIM analyses

Scientific Reports, 2022
Characterizing melanins in situ and determining their 3D z-epidermal distribution is paramount for understanding physiological/pathological processes of melanin neosynthesis, transfer, degradation or modulation with external UV exposure or cosmetic ...
Ana-Maria Pena, Etienne Decencière, Sébastien Brizion, Peggy Sextius, Serge Koudoro, Thérèse Baldeweck, Emmanuelle Tancrède-Bohin   +6 more
doaj   +1 more source

Imaging mitochondrial membrane potential via concentration-dependent fluorescence lifetime changes

Nature Communications
Mitochondria are central to cellular metabolism. Various fluorescence tools have been developed for imaging the mitochondrial environment. Yet, new reporters and imaging methods for directly reading the mitochondrial status are needed for high spatial ...
Dilizhatai Saimi, Luc Reymond, Tursunjan Aziz, Xuan Shen, Ziying Luo, Shuaibo Pi, Yitong Liu, Song Fu, Shuangjin Ding, Anming Meng, Liangyi Chen, Hui Jiang, Zhixing Chen   +12 more
doaj   +1 more source

Characterization of SPAD Array for Multifocal High-Content Screening Applications

Photonics, 2016
Current instruments used to detect specific protein-protein interactions in live cells for applications in high-content screening (HCS) are limited by the time required to measure the lifetime.
Anthony Tsikouras, Pietro Peronio, Ivan Rech, Nehad Hirmiz, M. Jamal Deen, Qiyin Fang   +5 more
doaj   +1 more source

Luminescent probes for luminescence lifetime sensing and imaging in live cells: a narrative review

Journal of Bio-X Research, 2020
. Luminescence (mostly fluorescence and phosphorescence) probes are a powerful tool widely used in the life sciences research. They can be used, for example, in the quantitative analysis of physiological parameters, visualization of different cell ...
Kaixuan Nie, Xiao Peng, Wei Yan, Jun Song, Junle Qu   +4 more
doaj   +1 more source

A fluorescence lifetime separation approach for FLIM live‐cell imaging

Journal of Microscopy
Abstract Fluorescence lifetime imaging microscopy (FLIM) translates the duration of excited states of fluorophores into lifetime information as an additional source of contrast in images of biological samples.
Cornelia Wetzker, Marcelo Leomil Zoccoler, Svetlana Iarovenko, Chukwuebuka William Okafornta, Anja Nobst, Hella Hartmann, Thomas Müller‐Reichert, Robert Haase, Gunar Fabig   +8 more
openaire   +2 more sources

Platycodon grandiflorus–Derived Nanovesicles Sensitize Gastric Cancer to T Cell Killing via Ferroptosis‐Driven Membrane Mechanical Remodeling

Advanced Science, EarlyView.
Plant‐derived nanovesicles from Platycodon grandiflorus act as bioactive lipid delivery platforms that transport γ‐linolenic acid into gastric cancer cells. This lipid cargo promotes ferroptotic lipid peroxidation, remodels membrane mechanics, and increases tumor‐cell susceptibility to cytotoxic T‐cell attack.
Jian Wu, Ruijuan Zhang, Yixin Duan, Shuqi Huo, Xiang Zhang, Yibin Xing, Lingjing Ma, Tiran Jiang, Xingyu Chen, Qingmin Sun   +9 more
wiley   +1 more source

Enhancing STED microscopy via fluorescence lifetime unmixing and filtering in two-species SPLIT-STED

Methods in Microscopy
Simultaneous super-resolution imaging of multiple fluorophores remains a major challenge in STimulated Emission Depletion (STED) microscopy due to spectral overlap of STED-compatible fluorophores.
Deschênes Andréanne, Ollier Antoine, Lafontaine Marie, Michaud-Gagnon Albert, Santiague Jeffrey-Gabriel Steavan, Bilodeau Anthony, Gagné Christian, De Koninck Paul, Lavoie-Cardinal Flavie   +8 more
doaj   +1 more source