Results 71 to 80 of about 6,539 (187)

Multispectral multiplexed confocal FLIM for live cell imaging

JPhys Photonics
Spectrally resolved fluorescence lifetime imaging (FLIM) with high spatial precision offers comprehensive information on species localization and behavior.
Morgan Richards, Elizabeth Julianna Osterlund, Andrea Rhikkaella Buendia, Nikolina Malic, Ray Truant, Qiyin Fang   +5 more
doaj   +1 more source

Investigating probe-receptor interactions and enhancing fluorescence guided surgery with fluorescence lifetime imaging and NF-800 in HER-2 positive esophageal adenocarcinoma

Scientific Reports
FLIM (Fluorescence Lifetime Imaging Microscopy) is a powerful technique used to study protein–protein interactions, enzyme kinetics dynamics, and cellular metabolism.
Francesca La Cava, Francesca Arena, Daniele Faletto, Margherita Iaboni, Federico Crivellin, Alessia Cordaro, Miriam Roberto, Federica Pozzi, Francesco Fersini, Giovanni Valbusa, Francesco Stummo, Mathieu Cinier, Simon Huet, Francesco Blasi, Alessandro Maiocchi, Luisa Poggi, Erika Reitano   +16 more
doaj   +1 more source

Long Fluorescence Lifetime Molecular Rotors Based on the 4,4‐Dicyano‐BODIPY Core

Chemistry – A European Journal, EarlyView.
Substituting fluorine with cyano groups in BODIPY‐based fluorescent molecular rotors yields viscosity‐sensitive dyes with significantly enhanced fluorescence lifetimes that can be used for viscosity mapping in living cells using FLIM microscopy. ABSTRACT Environmentally sensitive fluorescent probes with extended emission lifetimes are highly valuable ...
Yelyzaveta Denysieva, Ludovic Richert, Yves Mély, Julie Karpenko, Dmytro Dziuba   +4 more
wiley   +1 more source

Investigation of frequency-domain lifetime PSP technique using a fluorescence lifetime imaging (FLIM) camera

, 2018
Proceedings 18th International Symposium on Flow ...
Munekata, Mizue, Yorita, Daisuke, Henne, Ulrich, Klein, Christian, Holst, Gerhard   +4 more
openaire   +3 more sources

Gradient‐Structured Polymer Fiber Mats for Broadly Tunable Multicolor and White Lasing

Laser &Photonics Reviews, EarlyView.
Hierarchically engineered electrospun fiber mats containing separated red, green, and blue dyes enable highly tunable multicolor and white random lasing. Spatially controlled dye distribution suppresses energy transfer, allowing precise color adjustment across wide color gamuts and accurate reproduction of daylight white standards.
Alina Szukalska, Kinga Halicka‐Stępień, Jastin Popławski, Katarzyna Kołodzińska, Michal Majkowski, Joanna Cabaj, Lech Sznitko   +6 more
wiley   +1 more source

Rapid Acquisition of High-Pixel Fluorescence Lifetime Images of Living Cells via Image Reconstruction Based on Edge-Preserving Interpolation

Biosensors
Fluorescence lifetime imaging (FLIM) has established itself as a pivotal tool for investigating biological processes within living cells. However, the extensive imaging duration necessary to accumulate sufficient photons for accurate fluorescence ...
Yinru Zhu, Yong Guo, Xinwei Gao, Qinglin Chen, Yingying Chen, Ruijie Xiang, Baichang Lin, Luwei Wang, Yuan Lu, Wei Yan   +9 more
doaj   +1 more source

Use of Fluorescence Lifetime Imaging Microscopy (FLIM) as a Timer of Cell Cycle S Phase

PLOS ONE, 2016
Incorporation of thymidine analogues in replicating DNA, coupled with antibody and fluorophore staining, allows analysis of cell proliferation, but is currently limited to monolayer cultures, fixed cells and end-point assays. We describe a simple microscopy imaging method for live real-time analysis of cell proliferation, S phase progression over ...
Irina A. Okkelman, Ruslan I. Dmitriev, Tara Foley, Dmitri B. Papkovsky   +3 more
openaire   +5 more sources

Zero‐Dimensional Perovskite‐Inspired Scintillator‐Based High‐Radiation‐Resistant Radionuclide Analyzer Free From Irreversible Degradation

Laser &Photonics Reviews, EarlyView.
A zero‐dimensional perovskite‐inspired scintillator (Cs3Cu2I5) is developed as a high‐radiation‐resilient radionuclide analyzer capable of operating under extreme γ‐ray environments. Even after 200 kGy irradiation, the device fully restores its original spectroscopic performance through simple thermal annealing, demonstrating intrinsic defect ...
Jiwon Seo, Seungho Song, Youngjoo Park, Dakyoung Lee, Sang Hyun Nam, Chanho Kim, Young‐Hoon Kim, Kisung Lee, Beomjun Park, Jung‐Yeol Yeom   +9 more
wiley   +1 more source

Near‐infrared confocal imaging reveals the spatial organisation of far‐red photosynthesis in Shark Bay microbial mats and Halomicronema sp.

Journal of Microscopy, EarlyView.
Abstract Far‐red light (FRL) cyanobacteria occupy spectrally filtered habitats where visible wavelengths are depleted but far‐red radiation persists. Shark Bay (Western Australia) stromatolitic microbial mats provide such a habitat, containing subsurface cyanobacterial communities in which chlorophyll d/f (Chl d/f)‐containing phototrophs coexist with ...
Anya Salih, Katherina Petrou, Michael Johnson, Alexander M. Macmillan, Gareth Kindler, Brendan P. Burns, Unnikrishnan Kuzhiumparambil, Anthony W. D. Larkum   +7 more
wiley   +1 more source

Calcium‐activated chloride channels in pericytes and their role in regulating organ blood flow

The Journal of Physiology, EarlyView.
Abstract figure legend Pericytes are microvascular mural cells with diverse roles. Contractile pericytes directly regulate local perfusion, while non‐contractile pericytes coordinate upstream vascular contractility via propagating electrical signals.
Paolo Tammaro, Hikaru Hashitani
wiley   +1 more source