Results 91 to 100 of about 6,539 (187)

‘In silico labelling’: Exploring the biological landscape at the nanoscale using intelligent multimodal optical microscopy

Journal of Microscopy, Volume 304, Issue 1, Page 41-50, October 2026.
Abstract Multimodal optical microscopy has recently converged with artificial intelligence (AI) to enable in silico labelling—the computational prediction of fluorescence‐like molecular contrast from label‐free measurements. Fluorescence plays a crucial role in linking microscopy and spectroscopy at molecular level, enabling image formation by means of
A. Diaspro, P. Bianchini, R. Bizzarri, F. Cella Zanacchi, S. Civita, N. Incardona, L. Lanzanò, A. Morgantini, M. Salerno, L. Touijer   +9 more
wiley   +1 more source

Machine Learning Methods for Fluorescence Lifetime Imaging (FLIM) Based Label-Free Detection of Microglia. [PDF]

Front Neurosci, 2020
Sagar MAK, Cheng KP, Ouellette JN, Williams JC, Watters JJ, Eliceiri KW.   +5 more
europepmc   +1 more source

The SPL8‐YAB3/TCP19 module integrates transcriptional and protein–protein regulatory mechanisms to orchestrate anther development in Arabidopsis

The Plant Journal, Volume 128, Issue 1, October 2026.
SUMMARY SQUAMOSA PROMOTER BINDING PROTEIN‐LIKE 8 (SPL8) is a plant‐specific transcription factor essential for anther development, but its regulatory partners and downstream mechanisms remain largely unknown. We identified 26 SPL8‐interacting transcription factors by yeast two‐hybrid screening and bimolecular fluorescence complementation (BiFC) assays,
Tingting Li, Lei Zhang, Shuping Xing
wiley   +1 more source

Nanorulers for Super‐Resolution Microscopy

ChemBioChem, Volume 27, Issue 18, 28 September 2026.
Nanorulers serve as essential calibration standards to validate optical super‐resolution microscopy down to the Ångström scale. Complementary DNA‐, protein‐, and virus‐based scaffolds provide unique strategies to benchmark spatial resolution, evaluate quantitative labeling efficiency, and mini‐mize linkage errors.
Gerti Beliu, Alan M. Szalai, Guillermo Acuna, Alexey Chizhik, José Ignacio Gallea, Jörg Enderlein   +5 more
wiley   +1 more source

Fluorochrome separation by fluorescence lifetime phasor analysis in confocal and STED microscopy

Methods in Microscopy
In fluorescence microscopy, discrimination of fluorochromes in multi-color labeling was originally based on the emission spectrum only, then on emission and distinct excitation wavelengths.
Gonzalez Pisfil Mariano, Dietzel Steffen
doaj   +1 more source

Visualizing subcellular changes in the NAD(H) pool size versus redox state using fluorescence lifetime imaging microscopy of NADH

Communications Biology
NADH autofluorescence imaging is a promising approach for visualizing energy metabolism at the single-cell level. However, it is sensitive to the redox ratio and the total NAD(H) amount, which can change independently from each other, for example with ...
Angela Song, Nicole Zhao, Diana C. Hilpert, Caroline Perry, Joseph A. Baur, Douglas C. Wallace, Patrick M. Schaefer   +6 more
doaj   +1 more source

Multiplexed In Vivo Imaging with Fluorescence Lifetime‐Modulating Tags

Advanced Science
Fluorescence lifetime imaging microscopy (FLIM) opens new dimensions for highly multiplexed imaging in live cells and organisms using differences in fluorescence lifetime to distinguish spectrally identical fluorescent probes. Here, a set of fluorescence‐
Lina El Hajji, France Lam, Maria Avtodeeva, Hela Benaissa, Christine Rampon, Michel Volovitch, Sophie Vriz, Arnaud Gautier   +7 more
doaj   +1 more source

Fluorescence Lifetime Imaging Microscopy (FLIM) Data Analysis with TIMP

Journal of Statistical Software, 2007
Fluorescence Lifetime Imaging Microscopy (FLIM) allows fluorescence lifetime images of biological objects to be collected at 250 nm spatial resolution and at (sub-)nanosecond temporal resolution.
Sergey Laptenok, Katharine M. Mullen, Jan Willem Borst, Ivo H. M. van Stokkum, Vladimir V. Apanasovich, Antonie J. W. G. Visser   +5 more
doaj  

Fluorescence Lifetime Unmixing: A New Workflow for FLIM Live-Cell Imaging

ABSTRACT Fluorescence lifetime imaging microscopy (FLIM) translates the duration of excited states of fluorophores into lifetime information as additional source of contrast in images of biological samples. This offers the possibility to separate fluorophores particularly beneficial in case of similar excitation ...
Cornelia Wetzker, Marcelo Leomil Zoccoler, Svetlana Iarovenko, Chukwuebuka William Okafornta, Anja Nobst, Hella Hartmann, Thomas Müller-Reichert, Robert Haase, Gunar Fabig   +8 more
openaire   +1 more source