Results 81 to 90 of about 6,539 (187)

A robust method for autofluorescence-free immunofluorescence using high-speed fluorescence lifetime imaging microscopy

Scientific Reports
Autofluorescence from endogenous biomolecules presents a significant challenge in immunofluorescence microscopy, often severely hindering the detection of specific fluorescence signals.
Wonsang Hwang, Tyler McPartland, Sinyoung Jeong, Conor L. Evans   +3 more
doaj   +1 more source

Recent Trends of Fluorescence Lifetime Imaging Microscopy Analysis Using Machine Learning

Journal of Biomedical Photonics & Engineering
Fluorescence Lifetime Imaging Microscopy (FLIM) is an advanced imaging technique that provides quantitative information about molecular interactions and changes in the microenvironment by measuring the emission decay lifetimes of molecules undergoing ...
Ilya D. Shchechkin
doaj   +1 more source

Stimuli‐Responsive Hierarchical Structuring Controls Survival and Programs Hydrogelation of Supramolecular Metallofibers

Advanced Materials, Volume 38, Issue 56, 8 October 2026.
Aqueous Zn(II) Salphen metallofibers decorated with PNIPAM undergo reversible, multi‐stimuli‐responsive hierarchical bundling. This higher‐order structuring kinetically stabilizes the otherwise fragile assemblies against dilution, acidic hydrolysis, and transmetallation, enables selective sorting of responsive fibers, and programs hydrogelation at ...
Merlin R. Stühler, László Mérai, Kai Ludwig, Rainer Haag, Quinn A. Besford, Alex J. Plajer   +5 more
wiley   +1 more source

A Layered Two‐Dimensional Hydrogen‐Bonded Organic Framework Based on 2,5,8,11‐Tetrakis(4‐Carboxyphenyl)Perylene Diimide: Structural Nature, Optical and Photophysical Properties

Advanced Optical Materials, Volume 14, Issue 37, 9 October 2026.
A novel hydrogen‐bonded organic framework (HOF) based on perylene diimide (PDI) is synthesized, structurally and spectroscopically characterized. Its photophysical behavior highlights how supramolecular organizations govern the optical properties, including emission from monomers, aggregates, and excimer‐like species. Structural transformations induced
Mario de la Hoz Tomás, Taito Hashimoto, Boiko Cohen, Yuzuki Murata, Kazuki Shiga, Ryota Hanyu, Ryusei Oketani, Norimitsu Tohnai, Ichiro Hisaki, Abderrazzak Douhal   +9 more
wiley   +1 more source

A Universal Protein Ladder for Standardization of Diverse FRET Assays

Advanced Science, Volume 13, Issue 57, 14 October 2026.
Translating FRET measurements from in vitro to intracellular environments requires universal benchmarks. We present a modular protein ladder using TPR motifs to harmonize data across diverse platforms. This predictable calibration curve bridges single molecule FRET measurements, with cell based measurements including FLIM‐FRET.
Evelyn R. Smith, Katie L. Gelder, Lewis Hunter‐Craig, Daniel A. Bose, Timothy D. Craggs, Alison E. Twelvetrees   +5 more
wiley   +1 more source

Molecular Machine‐Driven Fluorogenic Probes for Motion–Signal Conversion and Hydrophilicity Retention in Live‐Cell Nanoscopy

Aggregate, Volume 7, Issue 10, October 2026.
A hydrophilicity‐retention strategy for developing fluorogenic probes by conjugating malachite green (as the molecular motion switch) with a spirocyclization‐free rhodamine (as the signal module) was developed for nanoscopic imaging of mitochondrial cristae structures and dynamics in living cells.
Yuan Luo, Lili Du, Xing Yang, Ke Liu, Chunbai Xiang, Yuanyuan Wang, Daoyong Jiang, Zihan Wu, Xiang‐Yang Lou, Xiaokang Zhang, Jiajie Diao, Xiaoshuai Huang, Ping Gong, Ben Zhong Tang, Lintao Cai, Pengfei Zhang   +15 more
wiley   +1 more source

[Fluorescence lifetime imaging microscopy (FLIM) in biological and medical research].

Postepy biochemii, 2010
Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for producing an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent sample. This technique can provide information, not only concerning the localization of specific fluorophores, but also about the local fluorophore environment. It can be
Jarosław, Korczyński, Jakub, Włodarczyk   +1 more
openaire   +1 more source

Unraveling Pathway Complexity in Photoreversible Supramolecular Hydrogels: Real‐Time Visualization and Sub‐Micrometric Photopatterning

Small, Volume 22, Issue 58, 16 October 2026.
This research demonstrates real‐time visualization of supramolecular sol‐gel transitions using Fluorescence Lifetime Imaging Microscopy (FLIM). By leveraging 5D visualization, integrating 3D space, time, and polarity resolution, we achieved maskless sub‐micrometric photopatterning within the gel matrix.
Federico Movilla, Consuelo Ripoll, Cristián Huck‐Iriart, Juan A. González‐Vera, Angel Orte, Rafael Contreras‐Montoya   +5 more
wiley   +1 more source

Neural Networks for Medical Data Processing: Segmentation of Fluorescence Lifetime Imaging Microscopy

Journal of Biomedical Photonics & Engineering
Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for visualizing the distribution of fluorescence lifetimes of fluorophores within a tissue, and is used for optical metabolic imaging.
Ilya Shchechkin, Svetlana Rodimova, Artem Mozherov, Vadim Elagin, Nikolai Bobrov, Vladislav Shcheslavsky, Vladimir Zagainov, Elena Zagaynova, Daria Kuznetsova   +8 more
doaj   +1 more source

A Triangulenum‐Based Biosensor Platform for Mapping Kinase Activity in T Cells

Small, Volume 22, Issue 58, 16 October 2026.
Novel peptide biosensors based on long‐lifetime triangulenium dyes are developed for the real‐time, non‐invasive mapping of Akt1 and Src kinase activities in living cells. Utilizing FLIM microscopy, these highly specific, biocompatible probes overcome cellular autofluorescence.
Carmen Salto‐Giron, Angélica Martín‐Priego, Francisco Fueyo‐González, María Mar Quesada‐Moreno, Federico Movilla, Antonio Reinoso, Laura Espinar‐Barranco, Rafael Salto, Amparo Navarro, Miguel Fribourg, Angel Orte, Juan A. González‐Vera   +11 more
wiley   +1 more source