Results 61 to 70 of about 6,539 (187)

Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy

Nature Communications, 2019
Nanosecond imaging techniques, such as fluorescence lifetime imaging microscopy (FLIM), are limited by low efficiency of current detectors. Here, the authors implement an electro-optic approach using Pockels cells for wide-field image gating and ...
Adam J. Bowman, Brannon B. Klopfer, Thomas Juffmann, Mark A. Kasevich   +3 more
doaj   +1 more source

Time-Domain Fluorescence Lifetime Imaging Techniques Suitable for Solid-State Imaging Sensor Arrays

Sensors, 2012
We have successfully demonstrated video-rate CMOS single-photon avalanche diode (SPAD)-based cameras for fluorescence lifetime imaging microscopy (FLIM) by applying innovative FLIM algorithms. We also review and compare several time-domain techniques and
Robert K. Henderson, Jochen Arlt, David Tyndall, Justin Richardson, Daniel R. Matthews, Viput Visitkul, Richard Walker, David Day-Uei Li, Simon Ameer-Beg   +8 more
doaj   +1 more source

Tracking CDK1 Dynamics With a Novel Biosensor Mouse and Clinical Cohorts to Predict CDK4/6 Inhibitor Sensitivity in ER+ Breast Cancer

Advanced Science, EarlyView.
Cyclin‐dependent kinase (CDK) 1 activity is a dynamic marker of treatment response in estrogen receptor positive breast cancer. Patient cohorts reveal CDK1 activity increases during progression to invasive disease, where high expression correlates with poor survival. Using a novel Cdk1 biosensor mouse alongside in vitro models, we demonstrate that CDK1
Sarah Alexandrou, Kristine J. Fernandez, Min Li Huang, Ewan K. A. Millar, Claire Vennin, Leila Eshraghi, Tan Dat Timothy Lam, Victoria Lee, Nyah C. Brooks, Janett Stoehr, Cristian Guaman Mendez, Douglas Strathdee, David Stevenson, David Herrmann, Sandra O'Toole, Paul Timpson, Max Nobis, C. Elizabeth Caldon   +17 more
wiley   +1 more source

Multifunctional Ionic Liquid Enabling Cation Homogenization and Lead Chelation for Pure‐Iodide Wide‐Bandgap Perovskite Solar Cells

Advanced Science, EarlyView.
This work presents a multifunctional ionic liquid, [SO3H‐PMIm][OTf], that addresses A‐site cation inhomogeneity and lead toxicity in pure‐iodide wide‐bandgap perovskite solar cells. The additive enables homogeneous cation distribution, achieving 22.02% efficiency with 90% retention after 3000 h and reducing lead leakage by over 58%, thereby ...
Kai Wu, Lei Yang, Guoqing Du, Xin Wang, Jiali Wei, Tiantian Li, Pengfei Zhang, Xiaodan Zhang, Fuhua Hou   +8 more
wiley   +1 more source

Mechanically Regulated Secretion: How Physical Forces Instruct the Secretory Pathway and Remodel the Secretome

Advanced Science, EarlyView.
Mechanical cues, including ECM stiffness, stretch, compression, shear stress, and traction forces, remodel trafficking across the secretory pathway, from the ER and Golgi to endolysosomal compartments and the plasma membrane. The resulting secretome reshapes ECM composition and tissue mechanics, establishing feedback loops that support homeostasis or ...
Domenico Russo, Seetharaman Parashuraman, Maria Luigia Maresca, Angela Francesca Dinoi, Alessia Calcagnì, Riccardo Rizzo   +5 more
wiley   +1 more source

Pinhole engineering based enhanced resolution (PEER) for fluorescence lifetime imaging microscopy

Communications Biology
In this article, we present a differential confocal microscopy technique based on pinhole engineering that significantly enhances both lateral resolution and axial sectioning depth.
Wonsang Hwang, Sinyoung Jeong, J. Matthew Dubach, Conor L. Evans, Iván Coto Hernández   +4 more
doaj   +1 more source

A Fluorogenic Cysteine‐Tetrapeptide Tagging Strategy for Nonperturbative Protein Labeling and Protein–Ligand Interaction Tracking

Advanced Science, EarlyView.
A compact Phe‐Cys‐Pro‐Phe (FCPF) tag and an environment‐sensitive SBD‐Cl‐derived probe form a sequence‐selective fluorogenic pair for wash‐free live‐cell protein imaging. This minimally perturbing chemistry allows flexible insertion at termini or loops, produces strong fluorescence upon local microenvironment confinement, supports FRET‐assisted ...
Xiao Wang, Xingcan Pan, Yongling Ying, Ziqian Bai, Jingming Zhang, Zhuang Shao, Jialiang Qian, Hongwei Li, Ning Zhang, Fan Yang, Yuan Liu, Xing Yang   +11 more
wiley   +1 more source

Alcohol‐Adduct Mediated Precursor Stabilization Strategies for Conformal SnO2 Layers in Highly Efficient and Stable Perovskite Solar Modules

Advanced Energy Materials, EarlyView.
An alcohol‐adduct‐mediated strategy is employed to stabilize tin isopropoxide for the formation of a homogeneous double electron transport layer (ETL) incorporating SnO2 nanoparticles (NPs). This double‐ETL exhibits improved optical and charge‐transport properties due to reduced defect density and better energy level alignment, resulting in high ...
You‐Hyun Seo, Eun Young Park, Chan Su Moon, Dong Gyu Lee, Junho Park, Fabian Rotermund, Gill Sang Han, Tae Kyung Lee, Nam Joong Jeon, Bong Joo Kang, Young Yun Kim   +10 more
wiley   +1 more source

A fluorescence lifetime-based FLIM-timer for measuring the protein turnover of transcription factor Nrf2 in live cells

Scientific Reports
Measuring protein turnover in cells has been greatly assisted by fluorescent timers (FT). However, FT quantification requires relatively high fluorescence intensity samples, prohibiting their use for proteins with low or non-uniform expression like ...
Dina Dikovskaya, Claudia Bento-Pereira, Kanade Shiga, Andrea Corno, Maureen Higgins, Rachel Toth, Adrian T. Saurin, Albena T. Dinkova-Kostova   +7 more
doaj   +1 more source

Surface‐Strained NiMo Cathodes Enable Kinetics‐Decoupled Hydrogen Bubble Management for Stable Near‐Neutral Water Electrolysis

Advanced Energy Materials, EarlyView.
Surface strain tunes the NiMo/electrolyte interface without altering intrinsic hydrogen evolution reaction (HER) kinetics. The strained surface exhibits a lower effective bubble‐detachment tendency and faster release of smaller H2 bubbles, sustaining a more accessible catalytic interface in near‐neutral electrolyte. Reduced bubble‐induced transport and
Si Eun Je, Sumin Lee, Seongjong Kim, Seyeong Jeong, Han Seo Im, Kyu‐Myung Lee, Seunggu Lee, Yongsup Park, Seongsoo Han, Seung‐Jae Shin, Wenjing Zhang, Donghoon Han, Sung Yul Lim   +12 more
wiley   +1 more source