Results 11 to 20 of about 30,206 (151)

Deconvolution of fluorescence lifetime imaging microscopy (FLIM) [PDF]

Multiphoton Microscopy in the Biomedical Sciences XXII, 2022
Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical micro-environment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two fluorophores within a diffraction limit are excited, the resulting emission leads to non-linear spatial distortion and ...
Mannam, Varun, Yuan, Xiaotong, Howard, Scott   +2 more
openaire   +2 more sources

Fluorescence Lifetime Imaging Microscopy of Biomolecular Condensates. [PDF]

Methods Mol Biol, 2023
Biomolecular condensates of ribonucleoproteins (RNPs) such as the transactivation response element (TAR) DNA-binding protein 43 (TDP-43) arise from liquid-liquid phase separation (LLPS) and play vital roles in various biological processes including the formation-dissolution of stress granules (SGs).
Quan MD, Liao SJ, Ferreon JC, Ferreon ACM.   +3 more
europepmc   +3 more sources

Practical guide to fluorescence lifetime imaging microscopy. [PDF]

Mol Biol Cell
Fluorescence lifetime imaging microscopy (FLIM) has emerged as a powerful modality that offers sensitivity to molecular environments, fluorophore concentration independence, local environment sensing, characterization of molecular binding events with multiplexing capabilities, and monitoring metabolism.
Sarfraz N, Braselmann E.
europepmc   +3 more sources

Advancements in fluorescence lifetime imaging microscopy Instrumentation: Towards high speed and 3D. [PDF]

Curr Opin Solid State Mater Sci
Park J, Gao L.
europepmc   +2 more sources

Fluorescence-Lifetime Imaging Techniques for Microscopy [PDF]

, 2003
CHAPTER 21 Fluorescence-Lifetime Imaging Techniques for Microscopy Chen Y. Dong, 1 Todd French, 2 Peter T. C. So, 1 C. Buehler, 1 Keith M. Berland, 3 and Enrico Gratton 1 Laboratory for Fluorescence Dynamics Department of Physics University of Illinois at Urbana-Champaign Urbana, Illinois 61801 Molecular Devices Corporation Sunnyvale, California 94089 ...
Dong, Chen Y, French, Todd, So, Peter TC, Buehler, C, Berland, Keith M, Gratton, Enrico   +5 more
openaire   +4 more sources

Advanced Data Analysis for Fluorescence-Lifetime Single-Molecule Localization Microscopy

Frontiers in Bioinformatics, 2021
Fluorescence-lifetime single molecule localization microscopy (FL-SMLM) adds the lifetime dimension to the spatial super-resolution provided by SMLM. Independent of intensity and spectrum, this lifetime information can be used, for example, to quantify ...
Jan Christoph Thiele, Oleksii Nevskyi, Dominic A. Helmerich, Markus Sauer, Jörg Enderlein, Jörg Enderlein   +5 more
doaj   +1 more source

Aggregation-induced emission luminogen for in vivo three-photon fluorescence lifetime microscopic imaging [PDF]

Journal of Innovative Optical Health Sciences, 2019
Compared with visible light, near-infrared (NIR) light has deeper penetration in biological tissues. Three-photon fluorescence microscopy (3PFM) can effectively utilize the NIR excitation to obtain high-contrast images in the deep tissue.
Huwei Ni, Zicong Xu, Dongyu Li, Ming Chen, Ben Zhong Tang, Jun Qian   +5 more
doaj   +1 more source

Fluorescence Lifetime Imaging Microscopy of Porphyrins in Helicobacter pylori Biofilms

Pharmaceutics, 2021
Bacterial biofilm constitutes a strong barrier against the penetration of drugs and against the action of the host immune system causing persistent infections hardly treatable by antibiotic therapy.
Antonella Battisti, Paola Morici, Antonella Sgarbossa   +2 more
doaj   +1 more source

Novel application of fluorescence lifetime and fluorescence microscopy enables quantitative access to subcellular dynamics in plant cells. [PDF]

PLoS ONE, 2009
BACKGROUND: Optical and spectroscopic technologies working at subcellular resolution with quantitative output are required for a deeper understanding of molecular processes and mechanisms in living cells.
Kirstin Elgass, Katharina Caesar, Frank Schleifenbaum, York-Dieter Stierhof, Alfred J Meixner, Klaus Harter   +5 more
doaj   +1 more source

Manipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding

Nature Communications, 2020
Using fluorescence lifetime as the readout modality offers more reproducible and quantitative outputs compared to conventional fluorescent barcoding, being independent of sample concentration and measurement methods.
Yujie Xie, Maria C. Arno, Jonathan T. Husband, Miquel Torrent-Sucarrat, Rachel K. O’Reilly   +4 more
doaj   +1 more source