Results 61 to 70 of about 79,470 (290)

Lifetime map reconstruction in frequency-domain fluorescence lifetime imaging microscopy [PDF]

2012 19th IEEE International Conference on Image Processing, 2012
We propose a robust statistical framework for reconstructing lifetime map corrupted by vesicle motion in frequency domain FLIM imaging. Instrumental noise is taken into account to improve lifetime estimation. Robust M-estimators and MLestimators allow to jointly estimate motion and lifetime. Performances are demonstrated on simulated and real samples.
Roudot, Philippe, Kervrann, Charles, Waharte, François, Boulanger, Jérôme   +3 more
openaire   +2 more sources

Color Routing and Beam Steering of Single‐Molecule Emission with a Spherical Silicon Nanoantenna

Advanced Functional Materials, EarlyView.
We experimentally demonstrate broadband directional emission from single molecules using a single spherical silicon nanoparticle assembled via DNA origami. By varying nanoparticle (NP) size and emitter position, we achieve unidirectional emission, beam steering, and color routing at the nanoscale, revealing modal interference as the underlying ...
María Sanz‐Paz, Nicole Siegel, Guillermo Serrera, Javier González‐Colsa, Fangjia Zhu, Karol Kołątaj, Minoru Fujii, Hiroshi Sugimoto, Pablo Albella, Guillermo P. Acuna   +9 more
wiley   +1 more source

Multiplexed In Vivo Imaging with Fluorescence Lifetime‐Modulating Tags

Advanced Science
Fluorescence lifetime imaging microscopy (FLIM) opens new dimensions for highly multiplexed imaging in live cells and organisms using differences in fluorescence lifetime to distinguish spectrally identical fluorescent probes. Here, a set of fluorescence‐
Lina El Hajji, France Lam, Maria Avtodeeva, Hela Benaissa, Christine Rampon, Michel Volovitch, Sophie Vriz, Arnaud Gautier   +7 more
doaj   +1 more source

Two-photon excitation fluorescent spectral and decay properties of retrograde neuronal tracer Fluoro-Gold

Scientific Reports, 2021
Fluoro-Gold is a fluorescent neuronal tracer suitable for targeted deep imaging of the nervous system. Widefield fluorescence microscopy enables visualization of Fluoro-Gold, but lacks depth discrimination.
Matthew Q. Miller, Iván Coto Hernández, Jenu V. Chacko, Steven Minderler, Nate Jowett   +4 more
doaj   +1 more source

J Fluorescence [PDF]

, 2005
The scope of this paper is to illustrate the need for an improved quality assurance in fluorometry. For this purpose, instrumental sources of error and their influences on the reliability and comparability of fluorescence data are highlighted for ...
Ebert, P., Engel, A., Hoffmann, K., Macdonald, R., Neukammer, J., Nietfeld, W., Nitschke, R., Resch-Genger, U.   +7 more
core   +2 more sources

Mechanochromism of Glassy Polymers Enabled by a Loop‐Forming Supramolecular Mechanophore

Advanced Functional Materials, EarlyView.
The supramolecular mechanophore LOOP is covalently introduced into glassy polymers, capable of reporting force‐induced pre‐failure damage and local fracture events in polymer glasses. Under different mechanical deformation modes, including grinding, tensile stretching, and scratching, LOOP probes irreversible events in polymer glasses through a ...
Linlin Deng, Chantal Graafsma, Felix Meyenhofer, Iulia Scarlat, Luca Bertossi, Christoph Weder, Jess M. Clough   +6 more
wiley   +1 more source

Dissecting aggregation and seeding dynamics of α-Syn polymorphs using the phasor approach to FLIM

Communications Biology, 2022
Fluorescence lifetime imaging microscopy combined with phasor analysis resolves seeding and aggregation dynamics of different α-synuclein polymorphs in a biosensor cell line.
Jessica Tittelmeier, Silke Druffel-Augustin, Ania Alik, Ronald Melki, Carmen Nussbaum-Krammer   +4 more
doaj   +1 more source

Fast fluorescence microscopy for imaging the dynamics of embryonic development [PDF]

, 2008
Live imaging has gained a pivotal role in developmental biology since it increasingly allows real-time observation of cell behavior in intact organisms.
Bakal C, Berg JM, Bewersdorf J, Born M, Brakenhoff GJ, Carpenter AE, Cecconi C, Chu KK, Egner A, Fraser S, Graf R, Hirose Y, Isalan M, Julien Vermot, Kam Z, Keller PJ, Kulesa PM, Kulesa PM, Lucitti JL, Mathis L, Michael Liebling, Murray JM, Niesner R, Nishiyama M, Nonaka S, Parsons SF, Potter SM, Rueckel M, Satpute‐Krishnan P, Scott E. Fraser, Sisson JH, Soutoglou E, Starkuviene V, Stathopoulos A, Stelzer EHK, Swedlow J, Tallini N, Tanaami T, Thomann D, Verveer PJ, Vonesch C, Wolleschensky R, Yang G, Zamir EA   +43 more
core   +3 more sources

Light Activated Induction of Cuproptosis in Resistant Cancer Cells Using Polymeric BODIPY Nanoparticles for Photoactivated Chemotherapy

Advanced Functional Materials, EarlyView.
This article presents a photo‐responsive nanoparticle platform that precisely triggers copper‐dependent cuproptotic cell death in (drug‐resistant) cancer cells. The system remains stable and inactive in the dark but releases cytotoxic species upon red‐light irradiation, achieving potent activity in drug‐resistant breast cancer cells.
Ricarda Zimmermann, Nicolás Montesdeoca, Johannes Karges   +2 more
wiley   +1 more source

The promise and peril of comparing fluorescence lifetime in biology revealed by simulations

eLife
Signaling dynamics are crucial in biological systems, and biosensor-based real-time imaging has revolutionized their analysis. Fluorescence lifetime imaging microscopy (FLIM) excels over the widely used fluorescence intensity imaging by allowing the ...
Pingchuan Ma, Peter Chen, Scott Sternson, Yao Chen   +3 more
doaj   +1 more source