Results 61 to 70 of about 30,206 (151)

Fluorochrome separation by fluorescence lifetime phasor analysis in confocal and STED microscopy

Methods in Microscopy
In fluorescence microscopy, discrimination of fluorochromes in multi-color labeling was originally based on the emission spectrum only, then on emission and distinct excitation wavelengths.
Gonzalez Pisfil Mariano, Dietzel Steffen
doaj   +1 more source

Recent Trends of Fluorescence Lifetime Imaging Microscopy Analysis Using Machine Learning

Journal of Biomedical Photonics & Engineering
Fluorescence Lifetime Imaging Microscopy (FLIM) is an advanced imaging technique that provides quantitative information about molecular interactions and changes in the microenvironment by measuring the emission decay lifetimes of molecules undergoing ...
Ilya D. Shchechkin
doaj   +1 more source

Multispectral multiplexed confocal FLIM for live cell imaging

JPhys Photonics
Spectrally resolved fluorescence lifetime imaging (FLIM) with high spatial precision offers comprehensive information on species localization and behavior.
Morgan Richards, Elizabeth Julianna Osterlund, Andrea Rhikkaella Buendia, Nikolina Malic, Ray Truant, Qiyin Fang   +5 more
doaj   +1 more source

Multiphoton Microscopy for Ophthalmic Imaging

Journal of Ophthalmology, 2011
We review multiphoton microscopy (MPM) including two-photon autofluorescence (2PAF), second harmonic generation (SHG), third harmonic generation (THG), fluorescence lifetime (FLIM), and coherent anti-Stokes Raman Scattering (CARS) with relevance to ...
Emily A. Gibson, Omid Masihzadeh, Tim C. Lei, David A. Ammar, Malik Y. Kahook   +4 more
doaj   +1 more source

Fluorophore multimerization on a PEG backbone as a concept for signal amplification and lifetime modulation

Scientific Reports
Fluorescent labels have strongly contributed to many advancements in bioanalysis, molecular biology, molecular imaging, and medical diagnostics. Despite a large toolbox of molecular and nanoscale fluorophores to choose from, there is still a need for ...
Thorge Reiber, Oskar Hübner, Christian Dose, Dmytro A. Yushchenko, Ute Resch-Genger   +4 more
doaj   +1 more source

Fluorescence Lifetime Imaging Microscopy [PDF]

, 2020
openaire   +1 more source

Advanced fluorescence lifetime-enhanced multiplexed nanoscopy of cells

Methods in Microscopy
In this review paper, we summarize the significant advancements in the field of fluorescence lifetime imaging microscopy (FLIM), particularly wide-field FLIM with single-molecule sensitivity, achieved using the time-correlated single-photon counting ...
Basak Samrat, Tsukanov Roman
doaj   +1 more source

Fluorescence lifetime image scanning microscopy

Single Molecule Spectroscopy and Superresolution Imaging XV, 2022
Gregor, Ingo, Rademacher, Niels, Tillmann, Max, Patting, Matthias, Enderlein, Jörg, Koberling, Felix, Gregor, Ingo, Erdmann, Rainer, Koberling, Felix   +8 more
openaire   +2 more sources

Emerging trends of fluorescence lifetime imaging microscopy (FLIM): advances, challenges, and prospects. [PDF]

Biophys Rep
Wang B, Li M, Huang X, Liu B.
europepmc   +1 more source

Introduction to the Special Issue on Fluorescence Lifetime Imaging Microscopy (FLIM) [PDF]

Journal of Innovative Optical Health Sciences, 2019
Junle Qu, Liwei Liu
doaj   +1 more source