Results 71 to 80 of about 79,470 (290)

PDT-Induced Variations of Radachlorin Fluorescence Lifetime in Living Cells In Vitro

Photonics, 2023
Variations in the fluorescence lifetimes of Radachlorin photosensitizers in HeLa and A549 cells, caused by photodynamic treatment, were studied using fluorescence lifetime imaging microscopy (FLIM).
Andrey V. Belashov, Anna A. Zhikhoreva, Anna V. Salova, Tatiana N. Belyaeva, Ilia K. Litvinov, Elena S. Kornilova, Irina V. Semenova   +6 more
doaj   +1 more source

Directed Molecular Stacking for Engineered Fluorescent Three-Dimensional Reduced Graphene Oxide and Coronene Frameworks [PDF]

, 2019
[EN] Three‐dimensional fluorescent graphene frameworks with controlled porous morphologies are of significant importance for practical applications reliant on controlled structural and electronic properties, such as organic electronics and photochemistry.
Botchway, S. W, Calatayud, D. G, Cortezon-Tamarit, F, Ge, Haobo, Kuganathan, Navaratnarajah, Köhn, Kociok, Mao, B, Mirabello, Vincenzo, Palomares, Francisco, Pascu, Sofia   +9 more
core   +1 more source

Photon Avalanching Nanoparticles: The Next Generation of Upconverting Nanomaterials?

Advanced Functional Materials, EarlyView.
This Perspective outlines the mechanistic foundations that enable photon‐avalanche (PA) behavior in lanthanide nanomaterials and contrasts them with emerging application spaces and forward‐looking design strategies. By bridging threshold engineering, energy‐transfer dynamics, and materials engineering, we provide a coherent roadmap for advancing the ...
Kimoon Lee, Yunseo Kim, Hyeong‐ku Jo, Dongjoon Yoon, Byeonghyeok Im, WonJeong Yu, Eui‐Rim On, Changhwan Lee   +7 more
wiley   +1 more source

Ruthenium polypyridyl peptide conjugates: membrane permeable probes for cellular imaging [PDF]

, 2008
Two novel polyarginine labelled ruthenium polypyridyl dyes are reported, one conjugated to five, (Ru-Ahx-R5), and one to eight arginine residues, (Ru-Ahx-R8). Both complexes exhibit long-lived, intense, and oxygen sensitive luminescence.
Amoroso, Aylward, Bai, Brunner, Dobrucki, Fischer, Fuchs, Fuchs, Fulda, Futaki, Kale, Kielar, Kielar, Lakowicz, Lissi, Lo, Marc Devocelle, Mendoza, Mendoza, Murray, Musatkinaa, Niamh Moran, Pellegrin, Robert J. Forster, Santra, Tia E. Keyes, Ute Neugebauer, William Signac, Xu, Yann Pellegrin, Zhang, Zhong, Zhong   +32 more
core   +1 more source

Global Analysis of Fluorescence Lifetime Imaging Microscopy Data [PDF]

Biophysical Journal, 2000
Global analysis techniques are described for frequency domain fluorescence lifetime imaging microscopy (FLIM) data. These algorithms exploit the prior knowledge that only a limited number of fluorescent molecule species whose lifetimes do not vary spatially are present in the sample. Two approaches to implementing the lifetime invariance constraint are
Verveer, P., Squire, A., Bastiaens, P.
openaire   +3 more sources

Disordered Carbon Shells Enable Shape Dependent Fluorescence Enhancement in Nanodiamond Quantum Sensors Revealed by Correlative TEM–Fluorescence Microscopy

Advanced Functional Materials, EarlyView.
Molten KNO2 treatment induces a lowrefractive index disordered carbon shell on fluorescent nanodiamonds, enhancing fluorescence emission while preserving spin coherence. Machine learningassisted correlative TEMPL enables direct single‐particle resolution of this enhancement relative to air‐oxidized nanodiamonds of similar morphology, establishing a ...
Parkarsh Kumar, Tom Vaillant, Mehran Kianinia, Haotian Wen, Adam Stewart, Peter J. Reece, Hugues A. Girard, Jean‐Charles Arnault, Huan‐Cheng Chang, Igor Aharonovich, Shery L. Y. Chang   +10 more
wiley   +1 more source

DNA‐Origami‐Assembled Rhodium Nanoantennas for Deep‐UV Label‐Free Single‐Protein Detection

Advanced Functional Materials, EarlyView.
Deep‐UV autofluorescence of single proteins in DNA origami‐based rhodium nanoantennas. Precisely positioned within the plasmonic nanogap, proteins retain their native state while their intrinsic emission is strongly amplified, enabling label‐free single‐molecule detection and opening new opportunities for highly sensitive and specific biosensing ...
Nicco Corduri, Malavika Kayyil Veedu, Yifan Yu, Yanqiu Zou, Jie Liu, Denis Garoli, Guillermo P. Acuna, Jérôme Wenger, Karol Kołątaj   +8 more
wiley   +1 more source

Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution

Nature Communications, 2021
High data volumes from multidimensional imaging techniques can lead to slow collection and processing times. Here, the authors implement multispectral fluorescence lifetime imaging microscopy (FLIM) that uses time-correlated photon counting technology to
Gareth O. S. Williams, Elvira Williams, Neil Finlayson, Ahmet T. Erdogan, Qiang Wang, Susan Fernandes, Ahsan R. Akram, Kev Dhaliwal, Robert K. Henderson, John M. Girkin, Mark Bradley   +10 more
doaj   +1 more source

Quantitative Imaging of Protein-Protein Interactions by Multiphoton Fluorescence Lifetime Imaging Microscopy using a Streak camera

, 2003
Fluorescence Lifetime Imaging Microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology.
A. Masuda, B. Herman, Becker, Clayton, Clegg, Cole, Elangovan, French, Gadella, Gerritsen, Harpur, Heya, Konig, Konig, Krishnan, Krishnan, Lakowicz, Mahajan, Neuberth, Oida, Periasamy, R. V. Krishnan, Schonle, Selvin, Squire, Squire, Straub, Sytsma, Szmacinski, Tahara, Tavare, V. E. Centonze, van Zandvoort, Verveer, Verveer, Verveer   +35 more
core   +1 more source

Bioanalysis using Fluorescence Lifetime Imaging Microscopy

Nippon Laser Igakkaishi, 2009
生体試料の分析において，蛍光寿命を用いてイメージングを行う方法が注目を集めている．蛍光強度は蛍光分子の濃度，励起光強度および光学系に依存するのに対し，蛍光寿命の値はこれらの実験条件に依存しないために，蛍光強度測定に比べて定量性を大きく増加させることができる．本稿では，蛍光寿命の特徴について概説した後，我々が製作した蛍光寿命イメージングシステムを用いた緑色蛍光タンパク質が発現した生細胞の測定結果について紹介する．
Takakazu Nakabayashi, Nobuhiro Ohta
openaire   +2 more sources