Results 21 to 30 of about 77,532 (261)

Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy: Concepts and Applications

Molecules, 2018
We review the basic concepts and recent applications of two-dimensional fluorescence lifetime correlation spectroscopy (2D FLCS), which is the extension of fluorescence correlation spectroscopy (FCS) to analyze the correlation of fluorescence lifetime in
Takuhiro Otosu, Shoichi Yamaguchi
doaj   +1 more source

Dead-time correction of fluorescence lifetime measurements and fluorescence lifetime imaging

Optics Express, 2016
We present a comprehensive theory of dead-time effects on Time-Correlated Single Photon Counting (TCSPC) as used for fluorescence lifetime measurements, and develop a correction algorithm to remove these artifacts. We apply this algorithm to fluorescence lifetime measurements as well as to Fluorescence Lifetime Imaging Microscopy (FLIM), where rapid ...
Isbaner, Sebastian, Karedla, Narain, Ruhlandt, Daja, Stein, Simon Christoph, Chizhik, Anna, Gregor, Ingo, Enderlein, Jörg   +6 more
openaire   +3 more sources

Novel application of fluorescence lifetime and fluorescence microscopy enables quantitative access to subcellular dynamics in plant cells. [PDF]

PLoS ONE, 2009
BACKGROUND: Optical and spectroscopic technologies working at subcellular resolution with quantitative output are required for a deeper understanding of molecular processes and mechanisms in living cells.
Kirstin Elgass, Katharina Caesar, Frank Schleifenbaum, York-Dieter Stierhof, Alfred J Meixner, Klaus Harter   +5 more
doaj   +1 more source

Fluorescence Lifetime Imaging of Apoptosis

Tomography, 2015
Genetically encoded fluorescence resonance energy transfer (FRET) reporters are powerful tools for analyzing cell signaling and function at single-cell resolution in standard 2D cell cultures, but these reporters rarely have been applied to 3D environments.
Xiao, Annie, Gibbons, Anne E., Luker, Kathryn E., Luker, Gary D.   +3 more
openaire   +2 more sources

Combining fluorescence lifetime with spectral information in fluorescence lifetime imaging ophthalmoscopy (FLIO)

Biomedical Optics Express, 2022
Fluorescence lifetime imaging ophthalmoscopy (FLIO) provides information on fluorescence lifetimes in two spectral channels as well as the peak emission wavelength (PEW) of the fluorescence. Here, we combine these measures in an integral three-dimensional lifetime-PEW metric vector and determine a normal range for this vector from measurements in young
Martin Hammer, Rowena Simon, Daniel Meller, Matthias Klemm   +3 more
openaire   +2 more sources

Photochromicity and Fluorescence Lifetimes of Green Fluorescent Protein [PDF]

The Journal of Physical Chemistry B, 1999
The green fluorescent protein (GFP) of the bioluminescent jellyfish Aequorea and its mutants have gained widespread usage as an indicator of structure and function within cells. Proton transfer has been implicated in the complex photophysics of the wild-type molecule, exhibiting a protonated A species excited at 400 nm, and two deprotonated excited ...
Striker, G., Subramaniam, V., Seidel, C., Volkmer, A.   +3 more
openaire   +2 more sources

Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy

The Scientific World Journal, 2013
Steady-state fluorence imaging and time-resolved fluorescence imaging are two important areas in fluorescence imaging research. Fluorescence lifetime imaging is an absolute measurement method which is independent of excitation laser intensity ...
Chao Liu, Xinwei Wang, Yan Zhou, Yuliang Liu   +3 more
doaj   +1 more source

Spatially resolved fluorescence of caesium lead halide perovskite supercrystals reveals quasi-atomic behavior of nanocrystals

Nature Communications, 2022
By utilizing spatially resolved fluorescence (-lifetime) measurements and high precision X-ray nanodiffraction, the authors correlate the influence of structural misalignment and fluorescence (-lifetime) properties of all-inorganic CsPbX3 (X– = Br–, Cl–)
Dmitry Lapkin, Christopher Kirsch, Jonas Hiller, Denis Andrienko, Dameli Assalauova, Kai Braun, Jerome Carnis, Young Yong Kim, Mukunda Mandal, Andre Maier, Alfred J. Meixner, Nastasia Mukharamova, Marcus Scheele, Frank Schreiber, Michael Sprung, Jan Wahl, Sophia Westendorf, Ivan A. Zaluzhnyy, Ivan A. Vartanyants   +18 more
doaj   +1 more source

Improved Protoporphyrin IX-Guided Neurosurgical Tumor Detection with Frequency-Domain Fluorescence Lifetime Imaging

Applied Sciences, 2022
Precise intraoperative brain tumor visualization supports surgeons in achieving maximal safe resection. In this sense, improved prognosis in patients with high-grade gliomas undergoing protoporphyrin IX fluorescence-guided surgery has been demonstrated ...
David Reichert, Mikael T. Erkkilae, Johanna Gesperger, Lisa I. Wadiura, Alexandra Lang, Thomas Roetzer-Pejrimovsky, Adelheid Woehrer, Marco Wilzbach, Christoph Hauger, Wolfgang Drexler, Barbara Kiesel, Georg Widhalm, Rainer A. Leitgeb, Angelika Unterhuber, Marco Andreana   +14 more
doaj   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source