Results 21 to 30 of about 207,530 (298)

Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy

Scientific Reports, 2021
We demonstrate that structured illumination microscopy has the potential to enhance fluorescence lifetime imaging microscopy (FLIM) as an early detection method for oral squamous cell carcinoma.
Taylor A. Hinsdale, Bilal H. Malik, Shuna Cheng, Oscar R. Benavides, Maryellen L. Giger, John M. Wright, Paras B. Patel, Javier A. Jo, Kristen C. Maitland   +8 more
doaj   +1 more source

Energy transfer from retinal to amino acids — a time-resolved study of the ultraviolet emission of bacteriorhodopsin [PDF]

, 1986
Two-step excitation of retinal in bacteriorhodopsin by visible light is followed by an energy transfer to amino acids that is seen as fluorescent emission around 350 nm.
Franz, M. A., Kaiser, Wolfgang, Oesterhelt, Dieter, Polland, Hans-Joachim, Zinth, Wolfgang   +4 more
core   +1 more source

Tapered optical fibers as tools for probing magneto-optical trap characteristics [PDF]

, 2009
We present a novel technique for measuring the characteristics of a magneto-optical trap for cold atoms by monitoring the spontaneous emission from trapped atoms coupled into the guided mode of a tapered optical nanofiber.
Chakrabarti, S., Chormaic, S. Nic, Deasy, K., Morrissey, M. J., Wu, Y.   +4 more
core   +2 more sources

Nonexponential statistics of fluorescence photobleaching [PDF]

, 2004
In this paper, I consider theoretical models of the decay via photobleaching of a sample of surface-immobilized fluorescent molecules excited by a spatially varying laser intensity profile.
Berglund, Andrew J.
core   +1 more source

Comparison of phasor analysis and biexponential decay curve fitting of autofluorescence lifetime imaging data for machine learning prediction of cellular phenotypes

Frontiers in Bioinformatics, 2023
Introduction: Autofluorescence imaging of the coenzymes reduced nicotinamide (phosphate) dinucleotide (NAD(P)H) and oxidized flavin adenine dinucleotide (FAD) provides a label-free method to detect cellular metabolism and phenotypes.
Linghao Hu, Blanche Ter Hofstede, Dhavan Sharma, Feng Zhao, Alex J. Walsh   +4 more
doaj   +1 more source

Manipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding

Nature Communications, 2020
Using fluorescence lifetime as the readout modality offers more reproducible and quantitative outputs compared to conventional fluorescent barcoding, being independent of sample concentration and measurement methods.
Yujie Xie, Maria C. Arno, Jonathan T. Husband, Miquel Torrent-Sucarrat, Rachel K. O’Reilly   +4 more
doaj   +1 more source

Fluorescence lifetime imaging through scattering media

Scientific Reports, 2023
Fluorescence lifetime determination has proven to be useful, e.g. identification of molecules, quantitative estimation of species concentration and determination of temperatures.
Sebastian Nilsson, Elias Kristensson, Marcus Aldén, Joakim Bood, Andreas Ehn   +4 more
doaj   +1 more source

Photophysics, Molecular Reorientation in Solution and X-Ray Structure of a New Fluorescent Probe 1,7-Diazaperylene [PDF]

, 1993
A new fluorescent molecule 1,7-diazaperylene (DP) has been investigated by means of time-resolved and steady-state polarized fluorescence spectroscopy, as well as X-ray spectroscopy.
Barkely, Bergelson, Birks, Chuang, Dawson, Demas, Ebeid, Ehrenberg, Heinz Langhals, Herbst, Jan Karolin, Johansson, Johansson, Kalman, Kalman, Kawski, Kurt Polborn, Langhals, Langhals, Langhals, Lennart B-Å. Johansson, Löhmannsröben, Melhuish, Naumann, Nicolai von Füner, Sadrai, Segur, Shilstone, Shinitzky, Susanne Reichherzer, Weber, Zannoni, Zinsli   +32 more
core   +1 more source

In Vivo Time Domain Optical Imaging of Renal Ischemia-Reperfusion Injury: Discrimination Based on Fluorescence Lifetime

Molecular Imaging, 2007
Fluorescence lifetime is an intrinsic parameter of the fluorescent probe, independent of the probe concentration but sensitive to changes in the surrounding microenvironment.
Abedelnasser Abulrob, Eric Brunette, Jacqueline Slinn, Ewa Baumann, Danica Stanimirovic   +4 more
doaj   +1 more source

How DASPMI reveals mitochondrial membrane potential : fluorescence decay kinetics and steady-state anisotropy in living cells [PDF]

, 2008
Spectroscopic responses of the potentiometric probe 2-(4-(dimethylamino)styryl)-1-methylpyridinium iodide (DASPMI) were investigated in living cells by means of a time- and space-correlated single photon counting technique.
Ramadass, Radhan, Bereiter-Hahn, Jürgen
core   +1 more source