Results 11 to 20 of about 207,530 (298)

Nanosecond-order long–short fluorescence lifetime switchable encryption with enlarged coding capacity

open access: yesNanophotonics, 2021
The turn-off fluorescent photoswitches for information encryption are constantly being developed. However, there are no reports about time-switchable (fluorescence lifetime-switchable) encryption to overcome the limitations of tunable encoding numbers in
Luo Teng   +3 more
doaj   +1 more source

PDT-Induced Variations of Radachlorin Fluorescence Lifetime in Living Cells In Vitro

open access: yesPhotonics, 2023
Variations in the fluorescence lifetimes of Radachlorin photosensitizers in HeLa and A549 cells, caused by photodynamic treatment, were studied using fluorescence lifetime imaging microscopy (FLIM).
Andrey V. Belashov   +6 more
doaj   +1 more source

Advanced Data Analysis for Fluorescence-Lifetime Single-Molecule Localization Microscopy

open access: yesFrontiers in Bioinformatics, 2021
Fluorescence-lifetime single molecule localization microscopy (FL-SMLM) adds the lifetime dimension to the spatial super-resolution provided by SMLM. Independent of intensity and spectrum, this lifetime information can be used, for example, to quantify ...
Jan Christoph Thiele   +5 more
doaj   +1 more source

Quantitative Imaging of Genetically Encoded Fluorescence Lifetime Biosensors

open access: yesBiosensors, 2023
Genetically encoded fluorescence lifetime biosensors have emerged as powerful tools for quantitative imaging, enabling precise measurement of cellular metabolites, molecular interactions, and dynamic cellular processes.
Cong Quang Vu, Satoshi Arai
doaj   +1 more source

A Review of New High-Throughput Methods Designed for Fluorescence Lifetime Sensing From Cells and Tissues

open access: yesFrontiers in Physics, 2021
Though much of the interest in fluorescence in the past has been on measuring spectral qualities such as wavelength and intensity, there are two other highly useful intrinsic properties of fluorescence: lifetime (or decay) and anisotropy (or polarization)
Aric Bitton   +3 more
doaj   +1 more source

Fluorescence lifetime imaging endoscopy [PDF]

open access: yesSPIE Proceedings, 2011
We present two FLIM endoscopes for clinical imaging and in vivo cell biology. For subcellular confocal imaging we demonstrated the first confocal FLIM endomicroscope, implementing TCSPC with a Cellvizio®GI, which we have now developed as a self-contained wheeled instrument (1.0 × 0.7 m) incorporating a tunable excitation laser and acquiring images ...
Kennedy, GT   +11 more
openaire   +3 more sources

Fluorescence Lifetime Imaging and Spectroscopic Co-Validation for Protoporphyrin IX-Guided Tumor Visualization in Neurosurgery

open access: yesFrontiers in Oncology, 2021
Maximal safe resection is a key strategy for improving patient prognosis in the management of brain tumors. Intraoperative fluorescence guidance has emerged as a standard in the surgery of high-grade gliomas.
David Reichert   +17 more
doaj   +1 more source

In vivo label-free mapping of the effect of a photosystem II inhibiting herbicide in plants using chlorophyll fluorescence lifetime

open access: yesPlant Methods, 2017
Background In order to better understand and improve the mode of action of agrochemicals, it is useful to be able to visualize their uptake and distribution in vivo, non-invasively and, ideally, in the field.
Elizabeth Noble   +5 more
doaj   +1 more source

ATP changes the fluorescence lifetime of cyan fluorescent protein via an interaction with His148. [PDF]

open access: yesPLoS ONE, 2010
Recently, we described that ATP induces changes in YFP/CFP fluorescence intensities of Fluorescence Resonance Energy Transfer (FRET) sensors based on CFP-YFP.
Jan Willem Borst   +7 more
doaj   +1 more source

Investigating photoexcitation-induced mitochondrial damage by chemotherapeutic corroles using multimode optical imaging [PDF]

open access: yes, 2012
We recently reported that a targeted, brightly fluorescent gallium corrole (HerGa) is highly effective for breast tumor detection and treatment.
Farkas, Daniel L.   +7 more
core   +1 more source

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