Results 11 to 20 of about 77,532 (261)

Fluorescence lifetime imaging [PDF]

Analytical Biochemistry, 1992
We describe a new fluorescence imaging methodology in which the image contrast is derived from the fluorescence lifetime at each point in a two-dimensional image and not the local concentration and/or intensity of the fluorophore. In the present apparatus, lifetime images are created from a series of images obtained with a gain-modulated image ...
J R, Lakowicz, H, Szmacinski, K, Nowaczyk, K W, Berndt, M, Johnson   +4 more
openaire   +2 more sources

Fluorescence Lifetime Imaging and Spectroscopic Co-Validation for Protoporphyrin IX-Guided Tumor Visualization in Neurosurgery

Frontiers in Oncology, 2021
Maximal safe resection is a key strategy for improving patient prognosis in the management of brain tumors. Intraoperative fluorescence guidance has emerged as a standard in the surgery of high-grade gliomas.
David Reichert, David Reichert, Mikael T. Erkkilae, Johanna Gesperger, Johanna Gesperger, Lisa I. Wadiura, Alexandra Lang, Thomas Roetzer, Adelheid Woehrer, Marco Andreana, Angelika Unterhuber, Marco Wilzbach, Christoph Hauger, Wolfgang Drexler, Barbara Kiesel, Georg Widhalm, Rainer A. Leitgeb, Rainer A. Leitgeb   +17 more
doaj   +1 more source

In vivo label-free mapping of the effect of a photosystem II inhibiting herbicide in plants using chlorophyll fluorescence lifetime

Plant Methods, 2017
Background In order to better understand and improve the mode of action of agrochemicals, it is useful to be able to visualize their uptake and distribution in vivo, non-invasively and, ideally, in the field.
Elizabeth Noble, Sunil Kumar, Frederik G. Görlitz, Chris Stain, Chris Dunsby, Paul M. W. French   +5 more
doaj   +1 more source

A Review of New High-Throughput Methods Designed for Fluorescence Lifetime Sensing From Cells and Tissues

Frontiers in Physics, 2021
Though much of the interest in fluorescence in the past has been on measuring spectral qualities such as wavelength and intensity, there are two other highly useful intrinsic properties of fluorescence: lifetime (or decay) and anisotropy (or polarization)
Aric Bitton, Jesus Sambrano, Samantha Valentino, Jessica P. Houston   +3 more
doaj   +1 more source

ATP changes the fluorescence lifetime of cyan fluorescent protein via an interaction with His148. [PDF]

PLoS ONE, 2010
Recently, we described that ATP induces changes in YFP/CFP fluorescence intensities of Fluorescence Resonance Energy Transfer (FRET) sensors based on CFP-YFP.
Jan Willem Borst, Marieke Willemse, Rik Slijkhuis, Gerard van der Krogt, Sergey P Laptenok, Kees Jalink, Be Wieringa, Jack A M Fransen   +7 more
doaj   +1 more source

Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy

Scientific Reports, 2021
We demonstrate that structured illumination microscopy has the potential to enhance fluorescence lifetime imaging microscopy (FLIM) as an early detection method for oral squamous cell carcinoma.
Taylor A. Hinsdale, Bilal H. Malik, Shuna Cheng, Oscar R. Benavides, Maryellen L. Giger, John M. Wright, Paras B. Patel, Javier A. Jo, Kristen C. Maitland   +8 more
doaj   +1 more source

Comparison of phasor analysis and biexponential decay curve fitting of autofluorescence lifetime imaging data for machine learning prediction of cellular phenotypes

Frontiers in Bioinformatics, 2023
Introduction: Autofluorescence imaging of the coenzymes reduced nicotinamide (phosphate) dinucleotide (NAD(P)H) and oxidized flavin adenine dinucleotide (FAD) provides a label-free method to detect cellular metabolism and phenotypes.
Linghao Hu, Blanche Ter Hofstede, Dhavan Sharma, Feng Zhao, Alex J. Walsh   +4 more
doaj   +1 more source

In Vivo Time Domain Optical Imaging of Renal Ischemia-Reperfusion Injury: Discrimination Based on Fluorescence Lifetime

Molecular Imaging, 2007
Fluorescence lifetime is an intrinsic parameter of the fluorescent probe, independent of the probe concentration but sensitive to changes in the surrounding microenvironment.
Abedelnasser Abulrob, Eric Brunette, Jacqueline Slinn, Ewa Baumann, Danica Stanimirovic   +4 more
doaj   +1 more source

Manipulating the fluorescence lifetime at the sub-cellular scale via photo-switchable barcoding

Nature Communications, 2020
Using fluorescence lifetime as the readout modality offers more reproducible and quantitative outputs compared to conventional fluorescent barcoding, being independent of sample concentration and measurement methods.
Yujie Xie, Maria C. Arno, Jonathan T. Husband, Miquel Torrent-Sucarrat, Rachel K. O’Reilly   +4 more
doaj   +1 more source

Fluorescence lifetime imaging through scattering media

Scientific Reports, 2023
Fluorescence lifetime determination has proven to be useful, e.g. identification of molecules, quantitative estimation of species concentration and determination of temperatures.
Sebastian Nilsson, Elias Kristensson, Marcus Aldén, Joakim Bood, Andreas Ehn   +4 more
doaj   +1 more source