Results 61 to 70 of about 9,845 (208)

Parallelized TCSPC for dynamic intravital fluorescence lifetime imaging: quantifying neuronal dysfunction in neuroinflammation.

PLoS ONE, 2013
Two-photon laser-scanning microscopy has revolutionized our view on vital processes by revealing motility and interaction patterns of various cell subsets in hardly accessible organs (e.g. brain) in living animals.
Jan Leo Rinnenthal, Christian Börnchen, Helena Radbruch, Volker Andresen, Agata Mossakowski, Volker Siffrin, Thomas Seelemann, Heinrich Spiecker, Ingrid Moll, Josephine Herz, Anja E Hauser, Frauke Zipp, Martin J Behne, Raluca Niesner   +13 more
doaj   +1 more source

Long Fluorescence Lifetime Molecular Rotors Based on the 4,4‐Dicyano‐BODIPY Core

Chemistry – A European Journal, EarlyView.
Substituting fluorine with cyano groups in BODIPY‐based fluorescent molecular rotors yields viscosity‐sensitive dyes with significantly enhanced fluorescence lifetimes that can be used for viscosity mapping in living cells using FLIM microscopy. ABSTRACT Environmentally sensitive fluorescent probes with extended emission lifetimes are highly valuable ...
Yelyzaveta Denysieva, Ludovic Richert, Yves Mély, Julie Karpenko, Dmytro Dziuba   +4 more
wiley   +1 more source

Early Detection of Lymph Node Metastasis Using Primary Head and Neck Cancer Computed Tomography and Fluorescence Lifetime Imaging

Diagnostics
Objectives: Early detection and accurate diagnosis of lymph node metastasis (LNM) in head and neck cancer (HNC) are crucial for enhancing patient prognosis and survival rates.
Nimu Yuan, Mohamed A. Hassan, Katjana Ehrlich, Brent W. Weyers, Garrick Biddle, Vladimir Ivanovic, Osama A. A. Raslan, Dorina Gui, Marianne Abouyared, Arnaud F. Bewley, Andrew C. Birkeland, D. Gregory Farwell, Laura Marcu, Jinyi Qi   +13 more
doaj   +1 more source

Metabolic fingerprinting of bacteria by fluorescence lifetime imaging microscopy

Scientific Reports, 2017
Bacterial populations exhibit a range of metabolic states influenced by their environment, intra- and interspecies interactions. The identification of bacterial metabolic states and transitions between them in their native environment promises to ...
Arunima Bhattacharjee, Rupsa Datta, Enrico Gratton, Allon I. Hochbaum   +3 more
doaj   +1 more source

Spatiotemporal cellular dynamics of the notochord shape intervertebral disc morphogenesis in the mouse embryo through apoptosis and proliferation

Developmental Dynamics, EarlyView.
Abstract Background The notochord is a midline structure essential for vertebrate embryogenesis, contributing to the development of the nervous system, digestive tract, and vertebral column. In particular, notochord signaling is indispensable for proper patterning and coordinated development of alternating vertebrae and intervertebral discs (IVDs ...
Julie Warin, Estelle Balissat, Pauline Colombier, Lily Paillat, Maeva Dutilleul, Anne Camus   +5 more
wiley   +1 more source

Overcoming photon and spatiotemporal sparsity in fluorescence lifetime imaging with SparseFLIM

Communications Biology
Fluorescence lifetime imaging microscopy (FLIM) provides quantitative readouts of biochemical microenvironments, holding great promise for biomedical imaging.
Binglin Shen, Yuan Lu, Fangyin Guo, Fangrui Lin, Rui Hu, Feng Rao, Junle Qu, Liwei Liu   +7 more
doaj   +1 more source

Finite Element Analysis for Li Batteries at the Mesoscale

ENERGY &ENVIRONMENTAL MATERIALS, EarlyView.
Finite element analysis (FEA), through multi‐physics coupling, has been widely employed to calculate mesoscale structures in LIBs that exhibit inherent randomness and disorder (Figure a). It has been extensively applied to the investigation of critical phenomena such as Li dendrite evolution, Li+ concentration gradients, SEI film growth, particle ...
Guanwu Li, Bo Gao, Dong Wang, Weitao Zheng   +3 more
wiley   +1 more source

Deep learning-based virtual H& E staining from label-free autofluorescence lifetime images

npj Imaging
Label-free autofluorescence lifetime is a unique feature of the inherent fluorescence signals emitted by natural fluorophores in biological samples. Fluorescence lifetime imaging microscopy (FLIM) can capture these signals enabling comprehensive analyses
Qiang Wang, Ahsan R. Akram, David A. Dorward, Sophie Talas, Basil Monks, Chee Thum, James R. Hopgood, Malihe Javidi, Marta Vallejo   +8 more
doaj   +1 more source

New constraints on the postglacial evolution of Lago Maggiore, Northern Italy

Journal of Quaternary Science, EarlyView.
ABSTRACT Lago Maggiore is situated in an overdeepened valley reach at the southern margin of the European Alps, and its thick sediment infill allows the reconstruction of the postglacial valley and lake evolution. We study here the sedimentary succession in the lower Ossola Valley, which is infilled by ~500 m of deposits. In a marginal position, a core
Lukas Gegg, Alexander Fülling, Andrew Greenwood, György Hetényi, F. Martin Hofmann, Giovanni Monegato, Jahir S. Rangel Herrera, Camille Schirra, Marco Venier, Othmar Müntener   +9 more
wiley   +1 more source

Interferometric excitation fluorescence lifetime imaging microscopy

Nature Communications
Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique with numerous imaging applications. Yet, one of the limitations of FLIM is that it only provides information about the emitting state.
Pavel Malý, Dita Strachotová, Aleš Holoubek, Petr Heřman   +3 more
doaj   +1 more source