Results 81 to 90 of about 36,842 (272)

Faster, sharper, more precise: Automated Cluster-FLIM in preclinical testing directly identifies the intracellular fate of theranostics in live cells and tissue

Theranostics, 2020
Fluorescence microscopy is widely used for high content screening in 2D cell cultures and 3D models. In particular, 3D tissue models are gaining major relevance in modern drug development.
Robert Brodwolf, Pierre Volz-Rakebrand, Johannes Stellmacher, Christopher Wolff, M. Unbehauen, R. Haag, M. Schäfer-Korting, C. Zoschke, U. Alexiev   +8 more
semanticscholar   +1 more source

Endoplasmic Reticulum Geometry Dictates Neuronal Bursting via Calcium Store Refill Rates and Exposes Selective Neuronal Vulnerability

Advanced Science, EarlyView.
The ER's continuous tubular network is maintained by ER‐shaping proteins whose mutation or dysregulation contributes to neurodegenerative diseases. Here, we show that ER morphology sets the speed of Ca2+ store replenishment between firing events. Disrupting ER continuity slows intra‐ER Ca2+ redistribution from extracellular refill (SOCE) sites, driving
Valentina Davi, Pierre Parutto, Yuyi Zhang, Tasuku Konno, Cecile Crapart, Raquel Pereira, John P. Franklin, Mosab Ali Awadelkareem, Daniel C Maddison, Michael J. Devine, Edgar R. Gomes, Joseph Chambers, Elena Koslover, Edward Avezov   +13 more
wiley   +1 more source

Fluorescence lifetime imaging microscopy and its applications in skin cancer diagnosis [PDF]

Journal of Innovative Optical Health Sciences, 2019
Fluorescence lifetime (FLT) of fluorophores is sensitive to the changes in their surrounding microenvironment, and hence it can quantitatively reveal the physiological characterization of the tissue under investigation.
Lixin Liu, Qianqian Yang, Meiling Zhang, Zhaoqing Wu, Ping Xue   +4 more
doaj   +1 more source

Molecular determinants of ER–Golgi contacts identified through a new FRET–FLIM system

Journal of Cell Biology, 2019
ER–TGN contact sites (ERTGoCS) have been visualized by electron microscopy, but their location in the crowded perinuclear area has hampered their analysis via optical microscopy as well as their mechanistic study.
Rossella Venditti, L. Rega, M. Masone, Michele Santoro, E. Polishchuk, Daniela Sarnataro, S. Paladino, S. d'Auria, A. Varriale, V. Olkkonen, G. di Tullio, R. Polishchuk, M. D. De Matteis   +12 more
semanticscholar   +1 more source

Fluorescence lifetime imaging microscopy (FLIM) detects differences in metabolic signatures between euploid and aneuploid human blastocysts.

Human Reproduction, 2020
STUDY QUESTION Can non-invasive imaging with fluorescence lifetime imaging microscopy (FLIM) detect metabolic differences in euploid versus aneuploid human blastocysts?
Jaimin S. Shah, M. Venturas, T. Sanchez, A. Penzias, D. Needleman, D. Sakkas   +5 more
semanticscholar   +1 more source

Piezo1 Channel Mediates Mechanically Programmable Drug Delivery to Potentiate Intravesical Chemotherapy

Advanced Science, EarlyView.
This study utilizes programmable mechanical pressure as a therapeutic enhancer to establish a mechano‐chemotherapy strategy. Controlled pressure activates the mechanosensitive ion channel Piezo1 in bladder cancer, triggering a calcium ion cascade that transiently and reversibly amplifies membrane permeability to chemotherapeutics.
Minghai Ma, Xing Li, Minxuan Jing, Zezhong Yang, Jiale He, Jianpeng Li, Xiao Liang, Yunzhong Jiang, Rou Huang, Yuanquan Zhang, Yuanchun Pu, Jiawei Xiu, Yutong Chen, Kaibo Mi, Pu Zhang, Lei Wang, Jinhai Fan   +16 more
wiley   +1 more source

Calibration approaches for fluorescence lifetime applications using time-domain measurements

Methods in Microscopy
This tutorial focuses on presenting experimental protocols for acquiring instrument response functions (IRF) and for calibrating the instruments using reference dyes with validated lifetime in time-domain fluorescence lifetime measurements.
Margineanu Anca
doaj   +1 more source

Fast fluorescence lifetime imaging techniques: A review on challenge and development [PDF]

Journal of Innovative Optical Health Sciences, 2019
Fluorescence lifetime imaging microscopy (FLIM) is increasingly used in biomedicine, material science, chemistry, and other related research fields, because of its advantages of high specificity and sensitivity in monitoring cellular microenvironments ...
Xiongbo Liu, Danying Lin, Wolfgang Becker, Jingjing Niu, Bin Yu, Liwei Liu, Junle Qu   +6 more
doaj   +1 more source

The Study of TeV Variability and Duty Cycle of Mrk 421 from 3 Years of Observations with the Milagro Observatory

, 2014
TeV flaring activity with time scales as short as tens of minutes and an orphan TeV flare have been observed from the blazar Markarian 421 (Mrk 421).
Abdo, A. A., Abeysekara, A. U., Allen, B. T., Aune, T., Barber, A. S., Berley, D., Braun, J., Chen, C., Christopher, G. E., Delay, R. S., DeYoung, T., Dingus, B. L., Ellsworth, R. W., Fraija, N., González, M. M., Goodman, J. A., Hays, E., Hoffman, C. M., Hüntemeyer, P. H., Imran, A., Kolterman, B. E., Linnemann, J. T., Marinelli, A., McEnery, J. E., Mincer, A. I., Morgan, T., Nemethy, P., Parkinson, P. M. Saz, Patricelli, B., Pretz, J., Ryan, J. M., Schneider, M., Shoup, A., Sinnis, G., Smith, A. J., Vasileiou, V., Walker, G. P., Williams, D. A., Yodh, G. B.   +38 more
core   +1 more source

Mapping metabolism of liver tissue using two-photon FLIM.

Biomedical Optics Express, 2020
Although fluorescence lifetime imaging microscopy (FLIM) has been extensively applied to study cellular metabolism in the liver, there is neither an established approach to analyze the data, nor have appropriate protocols been developed to maintain the ...
S. Rodimova, D. Kuznetsova, N. Bobrov, V. Elagin, V. Shcheslavskiy, V. Zagainov, E. Zagaynova   +6 more
semanticscholar   +1 more source