Results 81 to 90 of about 9,845 (208)

A robust method for autofluorescence-free immunofluorescence using high-speed fluorescence lifetime imaging microscopy

Scientific Reports
Autofluorescence from endogenous biomolecules presents a significant challenge in immunofluorescence microscopy, often severely hindering the detection of specific fluorescence signals.
Wonsang Hwang, Tyler McPartland, Sinyoung Jeong, Conor L. Evans   +3 more
doaj   +1 more source

A Layered Two‐Dimensional Hydrogen‐Bonded Organic Framework Based on 2,5,8,11‐Tetrakis(4‐Carboxyphenyl)Perylene Diimide: Structural Nature, Optical and Photophysical Properties

Advanced Optical Materials, Volume 14, Issue 37, 9 October 2026.
A novel hydrogen‐bonded organic framework (HOF) based on perylene diimide (PDI) is synthesized, structurally and spectroscopically characterized. Its photophysical behavior highlights how supramolecular organizations govern the optical properties, including emission from monomers, aggregates, and excimer‐like species. Structural transformations induced
Mario de la Hoz Tomás, Taito Hashimoto, Boiko Cohen, Yuzuki Murata, Kazuki Shiga, Ryota Hanyu, Ryusei Oketani, Norimitsu Tohnai, Ichiro Hisaki, Abderrazzak Douhal   +9 more
wiley   +1 more source

A Universal Protein Ladder for Standardization of Diverse FRET Assays

Advanced Science, Volume 13, Issue 57, 14 October 2026.
Translating FRET measurements from in vitro to intracellular environments requires universal benchmarks. We present a modular protein ladder using TPR motifs to harmonize data across diverse platforms. This predictable calibration curve bridges single molecule FRET measurements, with cell based measurements including FLIM‐FRET.
Evelyn R. Smith, Katie L. Gelder, Lewis Hunter‐Craig, Daniel A. Bose, Timothy D. Craggs, Alison E. Twelvetrees   +5 more
wiley   +1 more source

Molecular Machine‐Driven Fluorogenic Probes for Motion–Signal Conversion and Hydrophilicity Retention in Live‐Cell Nanoscopy

Aggregate, Volume 7, Issue 10, October 2026.
A hydrophilicity‐retention strategy for developing fluorogenic probes by conjugating malachite green (as the molecular motion switch) with a spirocyclization‐free rhodamine (as the signal module) was developed for nanoscopic imaging of mitochondrial cristae structures and dynamics in living cells.
Yuan Luo, Lili Du, Xing Yang, Ke Liu, Chunbai Xiang, Yuanyuan Wang, Daoyong Jiang, Zihan Wu, Xiang‐Yang Lou, Xiaokang Zhang, Jiajie Diao, Xiaoshuai Huang, Ping Gong, Ben Zhong Tang, Lintao Cai, Pengfei Zhang   +15 more
wiley   +1 more source

Müller Glia–Vasculature Interactions in the Developing Retina

Glia, Volume 74, Issue 10, October 2026.
Retinal vascular layers form normally despite loss of cholinergic retinal waves. Müller glial processes contact endothelial tip cells early and form Aquaporin‐4‐enriched endfeet. Glial endfoot calcium signals are compartmentalized and occur largely independently of neural activity. ABSTRACT Coordinated signaling among neurons, glia, and the vasculature
Samira Monshietehadi, Angel J. Garcia, Benjamin E. Smith, Matthew Tarchick, Kiara Abhayaratne, Aileen Conley, Marla B. Feller   +6 more
wiley   +1 more source

Comparative Genomic Analysis of Multidrug‐Resistant Escherichia coli Across Poultry–Human–Environmental Interfaces

MicrobiologyOpen, Volume 15, Issue 5, October 2026.
Whole‐genome sequencing analysis of Escherichia coli isolates from broiler, layer and sonali chickens in Bangladesh shown extensive antimicrobial resistance, diverse virulence factors, plasmid‐mediated resistance, and a highly open pangenome. Comparative genomic analyses demonstrated close genetic relationships with global strains, highlighting the One
Md. Rokibul Hasan Shanto, A. S. M. Ashab Uddin, Md. Shakil Mahmud Supto, Nabil Jahan Mahim, Md. Matiar Rahman Howlader, Syed Sayeem Uddin Ahmed, Md Bashir Uddin   +6 more
wiley   +1 more source

Multispectral multiplexed confocal FLIM for live cell imaging

JPhys Photonics
Spectrally resolved fluorescence lifetime imaging (FLIM) with high spatial precision offers comprehensive information on species localization and behavior.
Morgan Richards, Elizabeth Julianna Osterlund, Andrea Rhikkaella Buendia, Nikolina Malic, Ray Truant, Qiyin Fang   +5 more
doaj   +1 more source

Intrinsic Disorder as a New Frontier in Antimicrobial Resistance and Bacterial Fitness

MicrobiologyOpen, Volume 15, Issue 5, October 2026.
The works examines the link between intrinsic disorder in bacterial protein structure, and bacterial function, and antimicrobial resistance. It highlights how flexible, disordered proteins support secretion, stress responses, desiccation protection, and virulence, while also suggesting that these adaptable regions may provide novel targets for ...
Jack O' Callaghan, Michael P. Ryan, Sarah Hudson, Damien Thompson   +3 more
wiley   +1 more source

Unraveling Pathway Complexity in Photoreversible Supramolecular Hydrogels: Real‐Time Visualization and Sub‐Micrometric Photopatterning

Small, Volume 22, Issue 58, 16 October 2026.
This research demonstrates real‐time visualization of supramolecular sol‐gel transitions using Fluorescence Lifetime Imaging Microscopy (FLIM). By leveraging 5D visualization, integrating 3D space, time, and polarity resolution, we achieved maskless sub‐micrometric photopatterning within the gel matrix.
Federico Movilla, Consuelo Ripoll, Cristián Huck‐Iriart, Juan A. González‐Vera, Angel Orte, Rafael Contreras‐Montoya   +5 more
wiley   +1 more source

Investigating probe-receptor interactions and enhancing fluorescence guided surgery with fluorescence lifetime imaging and NF-800 in HER-2 positive esophageal adenocarcinoma

Scientific Reports
FLIM (Fluorescence Lifetime Imaging Microscopy) is a powerful technique used to study protein–protein interactions, enzyme kinetics dynamics, and cellular metabolism.
Francesca La Cava, Francesca Arena, Daniele Faletto, Margherita Iaboni, Federico Crivellin, Alessia Cordaro, Miriam Roberto, Federica Pozzi, Francesco Fersini, Giovanni Valbusa, Francesco Stummo, Mathieu Cinier, Simon Huet, Francesco Blasi, Alessandro Maiocchi, Luisa Poggi, Erika Reitano   +16 more
doaj   +1 more source