Results 91 to 100 of about 9,845 (208)

A Triangulenum‐Based Biosensor Platform for Mapping Kinase Activity in T Cells

Small, Volume 22, Issue 58, 16 October 2026.
Novel peptide biosensors based on long‐lifetime triangulenium dyes are developed for the real‐time, non‐invasive mapping of Akt1 and Src kinase activities in living cells. Utilizing FLIM microscopy, these highly specific, biocompatible probes overcome cellular autofluorescence.
Carmen Salto‐Giron, Angélica Martín‐Priego, Francisco Fueyo‐González, María Mar Quesada‐Moreno, Federico Movilla, Antonio Reinoso, Laura Espinar‐Barranco, Rafael Salto, Amparo Navarro, Miguel Fribourg, Angel Orte, Juan A. González‐Vera   +11 more
wiley   +1 more source

Rapid and precise multifocal cutaneous tumor margin assessment using fluorescence lifetime detection and machine learning

APL Photonics
The precise determination of surgical margins is essential for the management of multifocal cutaneous cancers, including extramammary Paget’s disease.
Wenhua Su, Dachao Zheng, Jiacheng Zhou, Qiushu Chen, Liwen Chen, Yuwei Yang, Yiyan Fei, Haijun Yao, Jiong Ma, Lan Mi   +9 more
doaj   +1 more source

‘In silico labelling’: Exploring the biological landscape at the nanoscale using intelligent multimodal optical microscopy

Journal of Microscopy, Volume 304, Issue 1, Page 41-50, October 2026.
Abstract Multimodal optical microscopy has recently converged with artificial intelligence (AI) to enable in silico labelling—the computational prediction of fluorescence‐like molecular contrast from label‐free measurements. Fluorescence plays a crucial role in linking microscopy and spectroscopy at molecular level, enabling image formation by means of
A. Diaspro, P. Bianchini, R. Bizzarri, F. Cella Zanacchi, S. Civita, N. Incardona, L. Lanzanò, A. Morgantini, M. Salerno, L. Touijer   +9 more
wiley   +1 more source

FRET-analysis in living cells by fluorescence lifetime imaging microscopy: experimental workflow and methodology

Methods in Microscopy
Fluorescence lifetime imaging microscopy (FLIM) combined with Förster resonance energy transfer (FRET) is a powerful tool for studying conformational changes of biosensors, protein-protein interactions and the dynamics of molecular processes in living ...
Weber André, Hartig Roland, Zuschratter Werner   +2 more
doaj   +1 more source

TipQuant: a robust algorithm for quantitative analysis of spatiotemporally dynamic activities in tip‐growing cells

New Phytologist, Volume 252, Issue 2, Page 926-935, October 2026.
Tip Quantification enables quantitative analyses over versatile selections of regions of interest. Summary Cell polarity and tip growth rely on the dynamic spatial organization of signaling and structural components. Quantitative characterization of these spatiotemporal dynamics is critical for understanding polarized cell growth, yet manual ...
Jingzhe Guo, Julian Le Gouic, Rémi Rosenthal, Ailing Zou, Xiang Zhou, Nicolas Brunel, Zhenbiao Yang, Xinping Cui   +7 more
wiley   +1 more source

The SPL8‐YAB3/TCP19 module integrates transcriptional and protein–protein regulatory mechanisms to orchestrate anther development in Arabidopsis

The Plant Journal, Volume 128, Issue 1, October 2026.
SUMMARY SQUAMOSA PROMOTER BINDING PROTEIN‐LIKE 8 (SPL8) is a plant‐specific transcription factor essential for anther development, but its regulatory partners and downstream mechanisms remain largely unknown. We identified 26 SPL8‐interacting transcription factors by yeast two‐hybrid screening and bimolecular fluorescence complementation (BiFC) assays,
Tingting Li, Lei Zhang, Shuping Xing
wiley   +1 more source

Carryover of Bracken Fern Toxins into Food, particularly into Water, Goat Milk and Goat Cheese from Swiss Alpine Farms

Food Risk Assess Europe, Volume 4, Issue 4, October 2026.
Abstract In recent years there has been growing interest in bracken toxins, in understanding their effect, occurrence and prevention. Most of these substances are glycosides and are therefore highly water soluble and mobile. They can transfer to drinking water or via livestock to milk and meat, thereby potentially posing a health risk.
Hans Reinhard, Barbara Engeli, Julie Tolu, Gisela Umbricht   +3 more
wiley   +1 more source

Nanorulers for Super‐Resolution Microscopy

ChemBioChem, Volume 27, Issue 18, 28 September 2026.
Nanorulers serve as essential calibration standards to validate optical super‐resolution microscopy down to the Ångström scale. Complementary DNA‐, protein‐, and virus‐based scaffolds provide unique strategies to benchmark spatial resolution, evaluate quantitative labeling efficiency, and mini‐mize linkage errors.
Gerti Beliu, Alan M. Szalai, Guillermo Acuna, Alexey Chizhik, José Ignacio Gallea, Jörg Enderlein   +5 more
wiley   +1 more source

Spectral scanning and fluorescence lifetime imaging microscopy (FLIM) enable separation and characterization of C. elegans autofluorescence in the cuticle and gut

Biology Open
Heino J. Hulsey-Vincent, Elizabeth A. Cameron, Caroline L. Dahlberg, Domenico F. Galati   +3 more
doaj   +1 more source

FLIM Networks with Bag of Feature Points

Accepted at the 28th Iberoamerican Congress on Pattern Recognition (CIARP 2025).
Martinelli, J.D., Rodrigues Filho, M.L., Salvagnini, F.C.D.R., Soares, G.J., Santos, J.A.D., Falcão, A.X.   +5 more
openaire   +3 more sources