Results 91 to 100 of about 1,049,609 (265)
A Poisson-Gaussian Denoising Dataset with Real Fluorescence Microscopy Images [PDF]
arXiv, 2018 Fluorescence microscopy has enabled a dramatic development in modern biology. Due to its inherently weak signal, fluorescence microscopy is not only much noisier than photography, but also presented with Poisson-Gaussian noise where Poisson noise, or shot noise, is the dominating noise source.
arxiv
Calibrating evanescent-wave penetration depths for biological TIRF microscopy
, 2019 Roughly half of a cells proteins are located at or near the plasma membrane. In this restricted space the cell senses its environment, signals to its neighbors and ex-changes cargo through exo- and endocytotic mechanisms.
Becherer, Ute +4 more
core +1 more source
Deconvolution in Fluorescence Lifetime imaging microscopy (FLIM) [PDF]
arXiv, 2022 Fluorescence lifetime imaging microscopy (FLIM) is an important technique to understand the chemical micro-environment in cells and tissues since it provides additional contrast compared to conventional fluorescence imaging. When two fluorophores within a diffraction limit are excited, the resulting emission leads to non-linear spatial distortion and ...
arxiv
Advances in Lensless Fluorescence Microscopy Design
PhotonicsLensless fluorescence microscopy (LLFM) has emerged as a promising approach for biological imaging, offering a simplified, high-throughput, portable, and cost-effective substitute for conventional microscopy techniques by removing lenses in favor of ...
Somaiyeh Khoubafarin +2 more
doaj +1 more source
Applied Microscopy, 2021 Intravital video microscopy permits the observation of microcirculatory blood flow. This often requires fluorescent probes to visualize structures and dynamic processes that cannot be observed with conventional bright-field microscopy. Conventional light
Barry G. H. Janssen +4 more
doaj +1 more source
LEDs for fluorescence microscopy
SPIE Proceedings, 2004 Traditional light sources for fluorescence microscopy have been mercury lamps, xenon lamps, and lasers. These sources have been essential in the development of fluorescence microscopy but each can have serious disadvantages: lack of near monochromaticity, heat generation, cost, lifetime of the light source, and possible distortions due to coherence ...
Young, I.T. (author) +4 more
openaire +4 more sources
Far-field fluorescence microscopy beyond the diffraction limit: Fluorescence imaging with ultrahigh resolution [PDF]
arXiv, 2007 Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution has stimulated research into developing methods to image beyond the diffraction limit based on far-field ...
arxiv
Detectors for Fluorescence Microscopy
, 2001 The low light levels originating from living cells viewed in the fluorescence microscope place significant limitations on the spatial and temporal resolution which can be achieved. The development of intensified video cameras has enabled the detection, visualization and measurement of these low level signals.
openaire +4 more sources
Interferometric excitation fluorescence lifetime imaging microscopy
Nature CommunicationsFluorescence lifetime imaging microscopy (FLIM) is a well-established technique with numerous imaging applications. Yet, one of the limitations of FLIM is that it only provides information about the emitting state.
Pavel Malý +3 more
doaj +1 more source
Adaptive particle representation of fluorescence microscopy images
Nature Communications, 2018 Modern microscopes can generate high volumes of 3D images, driving difficulties in data handling and processing. Here, the authors present a content-adaptive image representation as an alternative to standard pixels that goes beyond data compression to ...
Bevan L. Cheeseman +4 more
doaj +1 more source