Results 41 to 50 of about 1,049,609 (265)
Frontiers in fluorescence microscopy
The International Journal of Developmental Biology, 2009 How we see organisms and cells depends on the tools at our disposal. For over 150 years, biologists were forced to rely on fixed, dehydrated and stained specimens in order to guess how the living cells could function. It all changed abruptly during the last two decades when the rapid development of novel imaging techniques revolutionized the way ...
Rino, José +3 more
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Tip-Enhanced Fluorescence Microscopy at 10 Nanometer Resolution [PDF]
, 2004 We demonstrate unambiguously that the field enhancement near the apex of a laser-illuminated silicon tip decays according to a power law that is moderated by a single parameter characterizing the tip sharpness.
Gerton, Jordan M. +4 more
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Wide field fluorescence epi-microscopy behind a scattering medium enabled by speckle correlations [PDF]
Opt. Express 26, 9866-9881 (2018), 2018 Fluorescence microscopy is widely used in biological imaging, however scattering from tissues strongly limits its applicability to a shallow depth. In this work we adapt a methodology inspired from stellar speckle interferometry, and exploit the optical memory effect to enable fluorescence microscopy through a turbid layer.
arxiv +1 more source
Instant three-color multiplane fluorescence microscopy
Biophysical Reports, 2021 One of the most widely used microscopy techniques in biology and medicine is fluorescence microscopy, offering high specificity in labeling as well as maximal sensitivity. For live-cell imaging, the ideal fluorescence microscope should offer high spatial
Ingo Gregor +3 more
doaj
Correlative stochastic optical reconstruction microscopy and electron microscopy. [PDF]
PLoS ONE, 2015 Correlative fluorescence light microscopy and electron microscopy allows the imaging of spatial distributions of specific biomolecules in the context of cellular ultrastructure.
Doory Kim +5 more
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Light Sheet Fluorescence Microscopy Illuminating Soft Matter
Frontiers in Physics, 2021 Volumetric microscopic imaging data acquired at high speeds is often needed in studies of soft matter. Several microscopy techniques exist for this purpose, but a relative newcomer is light sheet fluorescence microscopy (LSFM). This microscopy method has
Ruilin You, Ryan McGorty
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Bridging fluorescence microscopy and electron microscopy [PDF]
Histochemistry and Cell Biology, 2008 Development of new fluorescent probes and fluorescence microscopes has led to new ways to study cell biology. With the emergence of specialized microscopy units at most universities and research centers, the use of these techniques is well within reach for a broad research community.
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Compressed sensing in fluorescence microscopy
Progress in Biophysics and Molecular Biology, 2022 Compressed sensing (CS) is a signal processing approach that solves ill-posed inverse problems, from under-sampled data with respect to the Nyquist criterium. CS exploits sparsity constraints based on the knowledge of prior information, relative to the structure of the object in the spatial or other domains.
Calisesi G. +6 more
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Elastohydrodynamic study of actin filaments using fluorescence microscopy [PDF]
, 1997 We probed the bending of actin subject to external forcing and viscous drag. Single actin filaments were moved perpendicular to their long axis in an oscillatory way by means of an optically tweezed latex bead attached to one end of the filaments. Shapes
Goldstein, Raymond E. +3 more
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Fourier imaging study of efficient near-field optical coupling in solid immersion fluorescence microscopy [PDF]
, 2002 We measured images and Fourier images of fluorescence for 0.11- and 0.22-$\mu$m-diameter dye-doped polystyrene micro-sphere beads on a solid immersion lens, and experimentally verified strongly-angle-dependent fluorescence intensities due to efficient near-field optical coupling in solid immersion fluorescence microscopy. The results are interpreted in
arxiv +1 more source