Results 31 to 40 of about 1,108,509 (287)

Elastohydrodynamic study of actin filaments using fluorescence microscopy [PDF]

open access: yes, 1997
We probed the bending of actin subject to external forcing and viscous drag. Single actin filaments were moved perpendicular to their long axis in an oscillatory way by means of an optically tweezed latex bead attached to one end of the filaments. Shapes
Goldstein, Raymond E.   +3 more
core   +3 more sources

Fluorescence Microscopy

open access: yes, 2013
Optical microscopy has developed as an indispensable tool for Arabidopsis cell biology. This is due to the high sensitivity, good spatial resolution, minimal invasiveness, and availability of autofluorescent proteins, which can be specifically fused to a distinct protein of interest.
Sébastien, Peter   +2 more
  +6 more sources

Scanless multitarget-matching multiphoton excitation fluorescence microscopy [PDF]

open access: yesJournal of Innovative Optical Health Sciences, 2018
Using the combination of a reflective blazed grating and a reflective phase-only diffractive spatial light modulator (SLM), scanless multitarget-matching multiphoton excitation fluorescence microscopy (SMTM-MPM) was achieved.
Junpeng Qiu   +4 more
doaj   +1 more source

Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations

open access: yesCells, 2021
Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell ...
Shane M. Hickey   +12 more
doaj   +1 more source

High performance, LED powered, waveguide based total internal reflection microscopy. [PDF]

open access: yes, 2013
Total internal reflection fluorescence (TIRF) microscopy is a rapidly expanding optical technique with excellent surface sensitivity and limited background fluorescence. Commercially available TIRF systems are either objective based that employ expensive
Cohen, Daniel A   +3 more
core   +1 more source

Epi-Fluorescence Microscopy [PDF]

open access: yes, 2012
Epi-fluorescence microscopy is available in most life sciences research laboratories, and when optimized can be a central laboratory tool. In this chapter, the epi-fluorescence light path is introduced and the various components are discussed in detail.
Donna J, Webb, Claire M, Brown
openaire   +2 more sources

Light Sheet Fluorescence Microscopy Illuminating Soft Matter

open access: yesFrontiers in Physics, 2021
Volumetric microscopic imaging data acquired at high speeds is often needed in studies of soft matter. Several microscopy techniques exist for this purpose, but a relative newcomer is light sheet fluorescence microscopy (LSFM). This microscopy method has
Ruilin You, Ryan McGorty
doaj   +1 more source

Correlative stochastic optical reconstruction microscopy and electron microscopy. [PDF]

open access: yesPLoS ONE, 2015
Correlative fluorescence light microscopy and electron microscopy allows the imaging of spatial distributions of specific biomolecules in the context of cellular ultrastructure.
Doory Kim   +5 more
doaj   +1 more source

Fluorescence antibunching microscopy

open access: yes, 2011
Breaking the diffraction limit in microscopy by utilizing quantum properties of light has been the goal of intense research in the recent years. We propose a quantum superresolution technique based on non-classical emission statistics of fluorescent ...
D. Oron, M. Kendall, O. Schwartz
core   +1 more source

Homologous Recombination under the Single-Molecule Fluorescence Microscope [PDF]

open access: yes, 2019
Homologous recombination (HR) is a complex biological process and is central to meiosis and for repair of DNA double-strand breaks. Although the HR process has been the subject of intensive study for more than three decades, the complex protein–protein ...
Dhakal, Soma, Gibbs, Dalton R.
core   +2 more sources

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