Results 11 to 20 of about 769,414 (303)
, 2019 In this two-part chapter, the background to confocal microscopy and two-photon fluorescence microscopy is first presented, with a detailed description of the optical setup.
Alberto Diaspro +15 more
core +5 more sources
Cold Spring Harbor Protocols, 2014 Fluorescence microscopy is a major tool with which to monitor cell physiology. Although the concepts of fluorescence and its optical separation using filters remain similar, microscope design varies with the aim of increasing image contrast and spatial resolution.
Sanderson, Michael J +3 more
openaire +4 more sources
Current Protocols in Immunology, 1998 AbstractAbsorption of energy as light by some molecules and emission of energy as fluorescence can occur only at certain wavelengths, which are characteristic for a given molecule (fluorophore). Fluorescence microscopes are equipped to observe the fluorescence of one or more specific fluorophores to localize specific molecules and analyzed cellular ...
Herman, Brian
core +4 more sources
Frontiers in Endocrinology, 2018 For a long time, the effects of distinct Eph tyrosine kinase receptors and their ligands, ephrins on the structure, immunophenotype, and development of thymus and their main cell components, thymocytes (T) and thymic epithelial cells (TECs), have been ...
Juan José Muñoz +6 more
doaj +1 more source
Evaluating performance in three-dimensional fluorescence microscopy [PDF]
, 2007 In biological fluorescence microscopy, image contrast is often degraded by a high background arising from out of focus regions of the specimen. This background can be greatly reduced or eliminated by several modes of thick specimen microscopy, including ...
JOHN M. MURRAY +7 more
core +1 more source
Multiple airy beams light-sheet fluorescence microscopy
Frontiers in Physics, 2022 Light-sheet fluorescence microscopy (LSFM) is a kind of volumetric imaging methodology suited for long term living specimens at high temporal-spatial resolution.
Shuangyu Gu +9 more
doaj +1 more source
Bleaching‐Resistant Super‐Resolution Fluorescence Microscopy
Advanced Science, 2022 Photobleaching is the permanent loss of fluorescence after extended exposure to light and is a major limiting factor in super‐resolution microscopy (SRM) that restricts spatiotemporal resolution and observation time.
Jiwoong Kwon +2 more
doaj +1 more source
Deep-learning-based methods for super-resolution fluorescence microscopy
Journal of Innovative Optical Health Sciences, 2023 The algorithm used for reconstruction or resolution enhancement is one of the factors affecting the quality of super-resolution images obtained by fluorescence microscopy. Deep-learning-based algorithms have achieved state-of-the-art performance in super-
Jianhui Liao +3 more
doaj +1 more source
Fluorescence Microscopy of the HIV-1 Envelope
Viruses, 2020 Human immunodeficiency virus (HIV) infection constitutes a major health and social issue worldwide. HIV infects cells by fusing its envelope with the target cell plasma membrane.
Pablo Carravilla +2 more
doaj +1 more source
Label-free 3D visualization of cellular and tissue structures in intact muscle with second and third harmonic generation microscopy. [PDF]
, 2011 Second and Third Harmonic Generation (SHG and THG) microscopy is based on optical effects which are induced by specific inherent physical properties of a specimen.
Krombach Fritz +11 more
core +1 more source