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Fluorescence Microscopy of the HIV-1 Envelope
Viruses, 2020 Human immunodeficiency virus (HIV) infection constitutes a major health and social issue worldwide. HIV infects cells by fusing its envelope with the target cell plasma membrane.
Pablo Carravilla +2 more
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Frontiers in Cell and Developmental Biology, 2022 Intracellular processes depend on a strict spatial and temporal organization of proteins and organelles. Therefore, directly linking molecular to nanoscale ultrastructural information is crucial in understanding cellular physiology.
Sergey V. Loginov +9 more
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Advanced Methods in Fluorescence Microscopy
Analytical Cellular Pathology, 2013 It requires a good deal of will power to resist hyperbole in considering the advances that have been achieved in fluorescence microscopy in the last 25 years.
Luke Fritzky, David Lagunoff
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Scanless multitarget-matching multiphoton excitation fluorescence microscopy [PDF]
Journal of Innovative Optical Health Sciences, 2018 Using the combination of a reflective blazed grating and a reflective phase-only diffractive spatial light modulator (SLM), scanless multitarget-matching multiphoton excitation fluorescence microscopy (SMTM-MPM) was achieved.
Junpeng Qiu +4 more
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Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations
Cells, 2021 Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell ...
Shane M. Hickey +12 more
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, 2013 Optical microscopy has developed as an indispensable tool for Arabidopsis cell biology. This is due to the high sensitivity, good spatial resolution, minimal invasiveness, and availability of autofluorescent proteins, which can be specifically fused to a distinct protein of interest.
Sébastien, Peter +2 more
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Light Sheet Fluorescence Microscopy Illuminating Soft Matter
Frontiers in Physics, 2021 Volumetric microscopic imaging data acquired at high speeds is often needed in studies of soft matter. Several microscopy techniques exist for this purpose, but a relative newcomer is light sheet fluorescence microscopy (LSFM). This microscopy method has
Ruilin You, Ryan McGorty
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Correlative stochastic optical reconstruction microscopy and electron microscopy. [PDF]
PLoS ONE, 2015 Correlative fluorescence light microscopy and electron microscopy allows the imaging of spatial distributions of specific biomolecules in the context of cellular ultrastructure.
Doory Kim +5 more
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Developing Digital Photomicroscopy
Cells, 2022 (1) The need for efficient ways of recording and presenting multicolour immunohistochemistry images in a pioneering laboratory developing new techniques motivated a move away from photography to electronic and ultimately digital photomicroscopy.
Kingsley Micklem
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Single-Molecule Clustering for Super-Resolution Optical Fluorescence Microscopy
Photonics, 2021 Molecular assembly in a complex cellular environment is vital for understanding underlying biological mechanisms. Biophysical parameters (such as single-molecule cluster density, cluster-area, pairwise distance, and number of molecules per cluster ...
Prakash Joshi, Partha Pratim Mondal
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