Results 41 to 50 of about 769,414 (303)

Wavefront shaping of a Bessel light field enhances light sheet microscopy with scattered light

, 2014
The project was supported by the UK Engineering and Physical Sciences Research Council, RS MacDonald Charitable Trust, SULSA, and the St. Andrews 600th anniversary BRAINS appeal. K. D.
Vettenburg, T.; id_orcid, J. Nylk, K. Dholakia, Nylk, J., C. Mitchell, Vettenburg, T., Gunn-Moore, F. J., T. Vettenburg, Mitchell, C., Dholakia, K., Nylk, J.; id_orcid, F. J. Gunn-Moore   +11 more
core   +1 more source

Applying two-photon excitation fluorescence lifetime imaging microscopy to study photosynthesis in plant leaves [PDF]

, 2009
This study investigates to which extent two-photon excitation (TPE) fluorescence lifetime imaging microscopy can be applied to study picosecond fluorescence kinetics of individual chloroplasts in leaves.
van Amerongen, H., Koen Broess, Amerongen, H., van, Jan Willem Borst, Borst, J.W., Broess, K., Herbert van Amerongen   +6 more
core   +1 more source

Fluorescence microscopy videos of mitochondria and endosomes in H9c2 cardiomyoblasts

, 2023
This dataset contains a series of three-dimensional fluorescence microscopy videos of the rat cardiomyoblast cell-line H9c2. The outer mitochondrial membrane is labeled with either mCherry (a red fluorescent protein) or eGFP (enhanced green fluorescent ...
Opstad, Ida
core   +1 more source

Two-photon laser scanning fluorescence microscopy using photonic crystal fibre

, 2004
We report the application of a simple yet powerful modular pulse compression system, based on photonic crystal fibres which improves upon incumbent twophoton laser scanning fluorescence microscopy techniques.
McConnell, G., Riis, E.
core   +1 more source

The Cytoscan (TM) model E-II, a new reflectance microscope for intravital microscopy: Comparison with the standard fluorescence method [PDF]

, 2000
The Cytoscan(TM) Model E-II (Cytometrics Inc., Philadelphia, Pa., USA) is a newly developed instrument which functions as an intravital microscope and is small and easily portable.
Sinitsina, I., Harris, A. G., Messmer, K.   +2 more
core   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Extracting fluorescent reporter time courses of cell lineages from high-throughput microscopy at low temporal resolution [PDF]

, 2011
The extraction of fluorescence time course data is a major bottleneck in high-throughput live-cell microscopy. Here we present an extendible framework based on the open-source image analysis software ImageJ, which aims in particular at analyzing the ...
Ott Sascha, Georgy Koentges, Mike J Downey, Jeziorska, DM, Tamai T. Katherine, Downey, Mike J., Downey Mike J., T. Katherine Tamai, Keith W, Bretschneider, T, Tamai, T Katherine, Downey, Mike J, Vance, KW, Ott, S, Mike J. Downey, Koentges, Georgy, Downey, MJ, Keith W Vance, Danuta M. Jeziorska, Bretschneider Till, Vance, Keith W., Jeziorska Danuta M., Till Bretschneider, Danuta M Jeziorska, Koentges Georgy, Sascha Ott, Vance Keith W., Koentges, G, T Katherine Tamai, Jeziorska, Danuta M, Bretschneider, Till, Jeziorska, Danuta M., Vance, Keith W, Ott, Sascha, Tamai, T. Katherine, Tamai, TK   +35 more
core   +1 more source

Frontiers in fluorescence microscopy

The International Journal of Developmental Biology, 2009
How we see organisms and cells depends on the tools at our disposal. For over 150 years, biologists were forced to rely on fixed, dehydrated and stained specimens in order to guess how the living cells could function. It all changed abruptly during the last two decades when the rapid development of novel imaging techniques revolutionized the way ...
Rino, José, Braga, José, Henriques, Ricardo, Carmo-Fonseca, Maria   +3 more
openaire   +4 more sources

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Single-Molecule Clustering for Super-Resolution Optical Fluorescence Microscopy

Photonics, 2021
Molecular assembly in a complex cellular environment is vital for understanding underlying biological mechanisms. Biophysical parameters (such as single-molecule cluster density, cluster-area, pairwise distance, and number of molecules per cluster ...
Prakash Joshi, Partha Pratim Mondal
doaj   +1 more source