Label-free 3D visualization of cellular and tissue structures in intact muscle with second and third harmonic generation microscopy. [PDF]
Second and Third Harmonic Generation (SHG and THG) microscopy is based on optical effects which are induced by specific inherent physical properties of a specimen.
Krombach Fritz +11 more
core +2 more sources
Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations
Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell ...
Shane M. Hickey +12 more
doaj +1 more source
Light Sheet Fluorescence Microscopy Illuminating Soft Matter
Volumetric microscopic imaging data acquired at high speeds is often needed in studies of soft matter. Several microscopy techniques exist for this purpose, but a relative newcomer is light sheet fluorescence microscopy (LSFM). This microscopy method has
Ruilin You, Ryan McGorty
doaj +1 more source
Correlative stochastic optical reconstruction microscopy and electron microscopy. [PDF]
Correlative fluorescence light microscopy and electron microscopy allows the imaging of spatial distributions of specific biomolecules in the context of cellular ultrastructure.
Doory Kim +5 more
doaj +1 more source
MUFASA: A Continuous-Time Stochastic Framework for Realistic Fluorescence Microscopy Simulation
We present MUFASA (Multi-Protocol Unified Fluorescence-based Advanced Simulation Algorithm), a physically grounded, continuous-time simulator for super-resolution fluorescence microscopy.
Blanc-Féraud, Laure +3 more
core +11 more sources
Fluorescence characterization of clinically-important bacteria [PDF]
Healthcare-associated infections (HCAI/HAI) represent a substantial threat to patient health during hospitalization and incur billions of dollars additional cost for subsequent treatment.
Jan-Peter Muller +25 more
core +2 more sources
Developing Digital Photomicroscopy
(1) The need for efficient ways of recording and presenting multicolour immunohistochemistry images in a pioneering laboratory developing new techniques motivated a move away from photography to electronic and ultimately digital photomicroscopy.
Kingsley Micklem
doaj +1 more source
3D volume rendering of invertebrates using light-sheet fluorescence microscopy [PDF]
Light-Sheet Fluorescence Microscopy (LSFM) has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms with low photo-damage and fast acquisition rates.
Gualda Manzano, Emilio José +1 more
core +1 more source
Wide-Field Multi-Parameter FLIM: Long-Term Minimal Invasive Observation of Proteins in Living Cells. [PDF]
Time-domain Fluorescence Lifetime Imaging Microscopy (FLIM) is a remarkable tool to monitor the dynamics of fluorophore-tagged protein domains inside living cells.
Yury Prokazov +20 more
core +2 more sources
Organoids in pediatric cancer research
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley +1 more source

